A direct and efficient method for derivatisation of solid supports for oligonucleotide synthesis

Tetrahedron Letters

Volumn 38, Issue 9, 1997, Pages 1651-1654

  Walsh, Andrew J ^a   Clark, G Claudine ^a   Fraser, William ^a  

^a ASTON UNIVERSITY   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

OLIGONUCLEOTIDE;

ARTICLE; DERIVATIZATION; DRUG SYNTHESIS; NUCLEOTIDE SEQUENCE; REACTION ANALYSIS;

EID: 0031550892     PISSN: 00404039     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0040-4039(97)00112-3     Document Type: Article

References (23)

1. 1. Abbreviations: DCC (N,N′-Dicyclohexylcarbodiimide), DCU (N,N′-Dicyclohexylurea), DhbtOH (3,4-Dihydro-3-hydroxy-4-oxo-1,2,3-benzotriazine), DMAP (4-Dimethylaminopyridine), DMF (Dimethylformamide), DTNP (2,2′-Dithiobis-(5-nitropyridine)), LCAA-CPG (Long Chain Alkyl Amine-Controlled Pore Glass), PNA (Peptidic Nucleic Acid), SLCPG (Silyl-Linked CPG), TEAA (Triethylammonium acetate), TPP (Triphenylphosphine).
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16. 5j
17. Bz, dT) and conventional, derivatised LCAA-CPG supports were purchased from Cruachem.
18. 9. User's Manual for Beckman 1000 DNA Synthesiser, 1994.
19. -1, was performed using an HPLC Technology C18 Reversed Phase Column (250 × 4.6 mm) with solvent system A mixed with solvent system B (0-40%) during 20 min, then with B (60%) during a further 10 min. A was composed of 1 M aqueous TEAA (10%) and MeCN (2%) at pH 7.0 and B was composed of 1 M aqueous TEAA (10%) and MeCN (80%) at pH 7.0.
20. 3 (aq) solution.
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