Structural and evolutionary studies on sterol 14-demethylase P450 (CYP51), the most conserved P450 monooxygenase: I. Structural analyses of the gene and multiple sizes of mRNA

Journal of Biochemistry

Volumn 122, Issue 6, 1997, Pages 1114-1121

  Noshiro, Mitsuhide ^a   Aoyama, Yuri ^b   Kawamoto, Takeshi ^a   Gotoh, Osamu ^c   Horiuchi, Tadao ^b   Yoshida, Yuzo ^d  

^a HIROSHIMA UNIVERSITY   (Japan)

^b SOKA UNIVERSITY   (Japan)

^c SAITAMA CANCER CENTER   (Japan)

^d MUKOGAWA WOMEN'S UNIVERSITY   (Japan)

Author keywords

Gene structure; P450; Processed pseudogene; Sterol 14 demethylase; Transcription initiation site

Indexed keywords

CYTOCHROME P450; MESSENGER RNA; POLYADENYLIC ACID; UNSPECIFIC MONOOXYGENASE;

ARTICLE; CONSENSUS SEQUENCE; EXON; FEMALE; GENE STRUCTURE; LIVER; MALE; NONHUMAN; OVARY; PROMOTER REGION; PSEUDOGENE; RAT; TESTIS; TRANSCRIPTION INITIATION; TRANSCRIPTION REGULATION;

RODENTIA;

EID: 0031473999     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.jbchem.a021869     Document Type: Article

References (26)

1. Yoshida, Y. (1993) Sterol biosynthesis in Cytochrome P450 (Schenkman, J.B. and Grein, H., eds.) pp. 627-639, Springer-Verlag, Berlin
2. Yoshida, Y. and Aoyama, Y. (1994) The P450 superfamily: A group of versatile hemoproteins contributing to the oxidation of various small molecules in Regulation of Heme Protein Synthesis (Fujita, H., ed.) pp. 75-88, AlphaMed Press, Dayton
3. Aoyama, Y., Noshiro, M., Gotoh, O., Imaoka, S., Funae, Y., Kurosawa, N., Horiuchi, T., and Yoshida, Y. (1996) Sterol 14-demethylase P450 (P45014DM) is one of the most ancient and conserved P450 species. J. Biochem. 119, 926-933
4. Gotoh, O. (1993) Evolution and differentiation of P-450 genes in Cytochrome P-450 (Omura, T., Ishimura, Y., and Fujii-Kuriyama, Y., eds.) pp. 207-223, Kodansha, Tokyo and VCH, Weinheim
5. Kalb, V.F., Woods, C.W., Turi, T.G., Dey, C.R., Sutter, T.R., and Loper, J.C. (1987) Primary structure of the P450 lanosterol demethylase gene from Saccharomyces cerevisiae. DNA 6, 529-537
6. Ishida, N., Aoyama, Y., Hatanaka, R., Oyama, Y., Imajo, S., Ishiguro, M., Oshima, T., Nakazato, H., Noguchi, T., Maitra, U.S., Mohan, V.P., Sprinson, D.S., and Yoshida, Y. (1988) A single amino acid substitution converts cytochrome P450(14DM) to an inactive form, cytochrome P450SG1: complete primary structures deduced from cloned DNAs. Biochem. Biophys. Res. Commun. 155, 317-323
7. Turi, T.G. and Loper, J.C. (1992) Multiple regulatory elements control expression of the gene encoding the Saccharomyces cerevisiae cytochrome P450, lanosterol 14α-demethylase (ERG-11). J. Biol. Chem. 267, 2046-2056
8. van Nistelrooy, J.G., van den Brink, J.M., van Kan, J.A., van Gorcom, R.F., and de Waard, M.A. (1996) Isolation and molecular characterization of the gene encoding eburicol 14α-demethylase (CYP51) from Penicillium italicum. Mol. Gen. Genet. 250, 725-733
9. Rozman, D., Stromstedt, M., and Waterman, M.R. (1996) The three human cytochrome P450 lanosterol 14α-demethylase (CYP51) genes reside on chromosomes 3, 7, and 13: structure of the two retrotransposed pseudogenes, association with a Line-1 element, and evolution of the human CYP51 family. Arch. Biochem. Biophys. 333, 466-474
10. Stromstedt, M., Rozman, D., and Waterman, M.R. (1996) The ubiquitously expressed human CYP51 encodes lanosterol 14α-demethylase, a cytochrome P450 whose expression is regulated by oxysterols. Arch. Biochem. Biophys. 329, 73-81
11. Cox, R.A. (1968) The use of guanidium chroride in the isolation of nucleic acids. Methods Enzymol. 12B, 120-129
12. Aviv, H. and Leder, P. (1972) Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose. Proc. Natl. Acad. Sci. USA 69, 1408-1412
13. Wong, G., Kawajiri, K., and Negishi, M. (1987) Gene family of male-specific testosterone 16α-hydroxylase (C-P-450(16)α) in mouse liver: cDNA sequences, neonatal imprinting, and reversible regulation by androgen. Biochemistry 26, 8683-8690
14. Frischauf, A.M., Lehrach, H., Poustka, A., and Murray, N. (1983) Lambda replacement vectors carrying polylinker sequences. J. Mol. Biol. 170, 827-842
15. Sambrook, J., Fritsch, E.F., and Maniatis, T. (1989) Molecular Cloning, 2nd ed., Cold Spring Harbor Laboratory, New York
16. Sanger, F., Coulson, A.R., Barrell, B.G., Smith, A.J., and Roe, B.A. (1980) Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing. J. Mol. Biol. 143, 161-178
17. Southern, E.M. (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol. 98, 503-517
18. Thomas, P.S. (1980) Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proc. Natl. Acad. Sci. USA 77, 5201-5205
19. Triezenberg, S.J. (1987) Primer extension in Current Protocols in Molecular Biology (Ausubel, F.M., Brent, R., Kingdston, R.E., Moore, D.D., Seidmen, J.G., Smith, J.A., and Struhl, K., eds.) Vol. 1, pp. 4.8.1-4.8.5, John Wiley & Sons, New York
20. Kalb, V.F., Glasser, S., King, D., and Lingrel, J.B. (1983) A cluster of repetitive elements within a 700 base pair region in the mouse genome. Nucleic Acids Res. 11, 2177-2184
21. Mount, S.M. (1982) A catalogue of splice junction sequences. Nucleic Acids Res. 10, 459-472
22. Rozman, D., Stromstedt, M., Tsui, L.-C., Scherer, S.W., and Waterman, M.R. (1996) Structure and mapping of the human lanosterol 14α-demethylase gene (CYP51) encoding the cytochrome P450 involved in cholesterol biosynthesis; comparison of exon/intron organization with other mammalian and fungal CYP genes. Genomics 38, 371-381
23. Yokoyama, C., Yabuki, T., Inoue, H., Tone, Y., Hara, S., Hatae, T., Nagata, M., Takahashi, E.-I., and Tanabe, T. (1996) Human gene encoding prostacyclin synthase (PTGIS): genomic organization, chromosomal localization, and promoter activity. Genomics 36, 296-304
24. Harada, N., Utsumi, T., and Takagi, Y. (1993) Tissue-specific expression of the human aromatase cytochrome P-450 gene by alternative use of multiple exons 1 and promoters, and switching of tissue-specific exons 1 in carcinogenesis. Proc. Natl. Acad. Sci. USA 90, 11312-11316
25. Kadonaga, J.T., Jones, K.A., and Tjian, R. (1986) Promoter-specific activation of RNA polymerase II transcription by SP1. Trends Biochem. Sci. 11, 20-23
26. Yoshida, Y., Yamashita, C., Noshiro, M., Fukuda, M., and Aoyama, Y. (1996) Sterol 14-demethylase P450 activity expressed in rat gonads: contribution to the formation of mammalian meiosis-activating sterol. Biochem. Biophys. Res. Commun. 223, 534-538