Recombination initiation: Easy as A, B, C, D...χ?

Current Biology

Volumn 7, Issue 12, 1997, Pages

  Eggleston, Angela K ^a   West, Stephen C ^a  

^a IMPERIAL CANCER RESEARCH FUND   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 0031470610     PISSN: 09609822     EISSN: None     Source Type: Journal    
DOI: 10.1016/s0960-9822(06)00394-0     Document Type: Review

References (16)

1. Myers RS, Stahl FW: χ and the RecBCD enzyme of Escherichia coli. Annu Rev Genet 1994, 28:49-70.
2. Bianco PR, Kowalczykowski SC: The recombination hotspot Chi is recognized by the translocating RecBCD enzyme as the single-strand of DNA containing the sequence 5′-GCTGGTGG-3′. Proc Natl Acad Sci USA 1997, 94:6706-6711.
3. Kowalczykowski SC, Dixon DA, Eggleston AK, Lauder SD, Rehrauer WM: Biochemistry of homologous recombination in Escherichia coli. Microbiol Revs 1994, 58:401-465.
4. Taylor AF, Schultz DW, Ponticelli AS, Smith GR: RecBC enzyme nicking at Chi sites during DNA unwinding: location and orientation-dependence of the cutting. Cell 1985, 41:153-163.
5. Dixon DA, Kowalczykowski SC: The recombination hotspot, χ, is a regulatory sequence that acts by attenuating the nuclease activity of the E. coli RecBCD enzyme. Cell 1993, 73:87-96.
6. Anderson DG, Kowalczykowski SC: The recombination hot spot χ is a regulatory element that switches the polarity of DNA degradation by the RecBCD enzyme. Genes Dev 1997, 11:571-581.
7. Taylor AF, Smith GR: Strand specificity of nicking of DNA at Chi sites by RecBCD enzyme: modulation by ATP and magnesium levels. J Biol Chem 1995, 270:24459-24467.
8. Anderson DG, Churchill JJ, Kowalczykowski SC: Chi-activated RecBCD enzyme possesses 5′→3′ nucleolytic activity, but RecBC enzyme does not: evidence suggesting that the alteration induced by Chi is not simply ejection of the RecD subunit. Genes Cells 1997, 2:117-128.
9. Dixon DA, Kowalczykowski SC: Homologous pairing in vitro stimulated by the recombination hotspot, Chi. Cell 1991, 66:361-371.
10. Anderson DG, Kowalczykowski SC: The translocating RecBCD enzyme stimulates recombination by directing RecA protein onto ssDNA in a χ-regulated manner. Cell 1997, 90:77-86.
11. Blattner FR, Plunkett G, Bloch CA, Perna NT, Burland V, Riley M, Colladovides J, Glasner JD, Rode CK, Mayhew GF, et al.: The complete genome sequence of Escherichia coli K-12. Science 1997, 277:1453-1474.
12. Tracy RB, Chédin F, Kowalczykowski SC: The recombination hot spot Chi is embedded within islands of preferred DNA pairing sequences in the E. coli genome. Cell 1997, 90:205-206.
13. Tracy RB, Kowalczykowski SC: In vitro selection of preferred DNA pairing sequences by the Escherichia coli RecA protein. Genes Dev 1996, 10:1890-1903.
14. Dixon DA, Churchill JJ, Kowalczykowski SC: Reversible inactivation of the Escherichia coli RecBCD enzyme by the recombination hotspot χ in vitro: evidence for functional inactivation or loss of the RecD subunit. Proc Natl Acad Sci USA 1994, 91:2980-2984.
15. Köppen A, Krobitsch S, Thoms B, Wackernagel W: Interaction with the recombination hot spot χ in vivo converts the RecBCD enzyme of Escherichia coli into a χ-independent recombinase by inactivation of the RecD subunit. Proc Natl Acad Sci USA 1995, 92:6249-6253.
16. Myers RS, Kuzminov A, Stahl FW: The recombination hot spot χ activates RecBCD recombination by converting Escherichia coli to a recD mutant phenocopy. Proc Natl Acad Sci USA 1995, 92:6244-6248.