Enriched SSCP: A highly sensitive method for the detection of unknown mutations. Application to the molecular diagnosis of lung cancer in sputum samples

Diagnostic Molecular Pathology

Volumn 6, Issue 4, 1997, Pages 185-191

  Marchetti, Antonio ^a   Buttitta, Fiamma ^a   Carnicelli, Vittoria ^a   Pellegrini, Silvia ^a   Bertacca, Gloria ^a   Merlo, Giorgio ^b   Bevilacqua, Generoso ^a  

^a UNIVERSITY OF PISA   (Italy)

^b ADVANCED BIOTECHNOLOGY CENTER   (Italy)

Author keywords

E SSCP; Lung cancer, diagnosis; Methods; Mutations; P53, p16

Indexed keywords

ALLELE; ARTICLE; CLINICAL ARTICLE; DIAGNOSTIC ACCURACY; DIFFERENTIAL DIAGNOSIS; GENE MUTATION; GENETIC LINKAGE; HUMAN; LUNG CANCER; LUNG METASTASIS; PRIORITY JOURNAL; SINGLE STRAND CONFORMATION POLYMORPHISM; SPUTUM CYTODIAGNOSIS; TUMOR SUPPRESSOR GENE;

DNA MUTATIONAL ANALYSIS; DNA PRIMERS; DNA, NEOPLASM; GENES, P53; HUMANS; LUNG NEOPLASMS; MUTATION; POLYMERASE CHAIN REACTION; POLYMORPHISM, SINGLE-STRANDED CONFORMATIONAL; SENSITIVITY AND SPECIFICITY; SPUTUM;

EID: 0031470552     PISSN: 10529551     EISSN: None     Source Type: Journal    
DOI: 10.1097/00019606-199708000-00002     Document Type: Article

References (23)

1. Blin N, Stafford DW. A general method for isolation of high molecular weight DNA from eucaryotes. Nucleic Acid Res 1976; 3:2303-08.
2. Bosari S, Marchetti A, Buttitta F, et al. Detection of p53 mutations by single-strand conformation polymorphism (SSCP) gel electrophoresis: a comparative study of radioactive and non-radioactive silver-stained SSCP analysis. Diagn Mol Pathol 1995;4:249-55.
3. Buttitta F, Marchetti A, Gadducci A, et al. p53 alterations are predictive of chemoresistance and aggressiveness in ovarian carcinomas: a molecular and immunohistochemical study. Br J Cancer 1997;75:230-35.
4. Caldas C, Hahn SA, Hrubam RH, Redston MS, Yeo CJ, Kern SE. Detection of K-ras mutations in the stool of patients with pancreatic adenocarcinoma and pancreatic ductal hyperplasia. Cancer Res 1994;54:3568-73.
5. Hayashi K, Yandel DW. How sensitive is PCR-SSCP? Hum Mutat 1993;2:238-46.
6. Hayashi N, Arakawa H, Nagase H, et al. Genetic diagnosis identifies occult lymph node metastases undetectable by the histopathological method. Cancer Res 1994;54:3853-56.
7. Hruban RH, van der Riet P, Erozan YS, Sidranski D. Brief report: molecular biology and the early detection of carcinoma of the bladder - the case of Hubert H. Humphrey. N Engl J Med 1994; 330:1276-78.
8. Jacobson DR, Mills NE. A highly sensitive assay for mutant ras genes and its application to the study of presentation and relapse genotypes in acute leukemia. Oncogene 1994;9:553-63.
9. Kondo H, Sugano K, Fukayama N, et al. Detection of point mutations in the K-ras oncogene at codon 12 in pure pancreatic juice for diagnoses of pancreatic carcinoma. Cancer 1993;73:1589-94.
10. Mao L, Hruban RH, Boyle JO, Tockman M, Sidransky D. Detection of oncogene mutations in sputum precedes diagnosis of lung cancer. Cancer Res 1994;54:1634-37.
11. Marchetti A, Buttitta F, Merlo G, et al. p53 alterations in non-small cell lung cancer correlate with metastatic involvement of hilar and mediastinal lymph nodes. Cancer Res 1993;53:2846-51.
12. Marchetti A, Buttitta F, Pellegrini S, et al. p53 mutations and histologic type of invasive breast carcinomas. Cancer Res 1993; 53:4665-69.
13. Marchetti A, Buttitta F, Pellegrini S, et al. p53 mutations in breast carcinoma. A polymerase chain reaction-single-strand conformation polymorphism analysis. Ann NY Acad Sci 1993;949:114-19.
14. Marchetti A, Merlo G, Buttitta F, Pellegrini S, Callahan R, Bistocchi M, Squartini F. Detection of DNA mutations in acid formaline fixed-paraffin embedded specimens by polymerase chain reaction-single strand conformation polymorphysm analysis. Cancer Det Prev 1995;19:278-81.
15. Marchetti A, Buttitta F, Pellegrini S, Lori A, Bertacca G, Bevilacqua G. Absence of somatic mutations in the coding region of the WAF1/CIP1 gene in lung, breast and ovarian carcinomas. A polymorphism at codon 31. Int J Oncol 1995;6:187-89.
16. Marchetti A, Buttitta F, Pellegrini S, Campani D, Cecchetti D, Bistocchi M. p53 and c-ErbB-2 alterations in "in situ" and invasive ductal breast carcinomas: a genetic and immunohistochemical study. Int J Oncol 1995;7:343-47.
17. Marchetti A, Buttitta F, Pellegrini S, et al. Alterations of p16 (mts 1) in node positive non-small cell lung carcinomas. J Pathol 1997; 181:178-82.
18. Murukami Y, Hayashi K, Sekiya T. Detection of aberrations of the p53 alleles and the gene transcript in human tumor cell lines by single-strand conformation polymorphism analysis. Cancer Res 1991;51:3356-61.
19. Sheffield VC, Beck JS, Kwitek AE, Sandstrom DW, Stone EM. The sensitivity of single strand conformation polymorphism analysis for the detection of single base substitution. Genomics 1993; 16:325-32.
20. Sidransky D, Von Eschenbach A, Tsai YC, et al. Identification of p53 gene mutations in bladder cancers and urine samples. Science 1991;252:706-09.
21. Sidransky D, Tokino T, Hamilton SR, Kinzler KW, Levin B, Frost P, Vogelstein B. Identification of ras ancogene mutations in the stool of patients with curable colorectal tumors. Science 1992;256: 102-05.
22. Slebos RJC, Boerrigter L, Evers SG, Wisman P, Mooi WJ, Rodenhuis S. A rapid and simple procedure for the routine detection of ras mutations in formalin-fixed, paraffin-embedded tissues. Diagn Mol Pathol 1992;1:136-41.
23. Tada M, Omata M, Kawai S, Saisho H, Saiki RK, Sninsky JJ. Detection of ras gene mutations in pancreatic juice and peripheral blood of patients with pancreatic adenocarcinoma. Cancer Res 1993;53:2472-74.