Biochemical and functional characterization of recombinant von Willebrand factor produced on a large scale

Cellular and Molecular Life Sciences

Volumn 53, Issue 11-12, 1997, Pages 943-950

  Fischer, B E ^a   Schlokat, U ^a   Reiter, M ^a   Mundt, W ^a   Dorner, F ^a  

^a BAXTER HEALTHCARE CORPORATION   (United States)

Author keywords

Coagulation factor VIII; Collagen binding; Glycosylation; Platelet aggregation; Platelet binding; Recombinant protein; von Willebrand factor

Indexed keywords

BLOOD CLOTTING FACTOR 8; COLLAGEN; GALACTOSE; GLUCOSE; HEPARIN; LECTIN; MANNOSE; N ACETYLGLUCOSAMINE; POLYMER; RECOMBINANT BLOOD CLOTTING FACTOR 8; RECOMBINANT PROTEIN; RISTOCETIN; SIALIC ACID; VON WILLEBRAND FACTOR;

AFFINITY CHROMATOGRAPHY; ANIMAL CELL; ANION EXCHANGE CHROMATOGRAPHY; ARTICLE; CHO CELL; CONTROLLED STUDY; DRUG BINDING; DRUG SYNTHESIS; FERMENTATION; KINETICS; NONHUMAN; PROTEIN BINDING; PROTEIN DENATURATION; PROTEIN PROTEIN INTERACTION; STOICHIOMETRY; THROMBOCYTE; THROMBOCYTE AGGREGATION;

ANIMALIA; CRICETINAE; CRICETULUS GRISEUS;

EID: 0031466198     PISSN: 1420682X     EISSN: None     Source Type: Journal    
DOI: 10.1007/s000180050115     Document Type: Article

References (45)

1. Ruggeri Z. M. and Ware J. (1992) The structure and function of von Willebrand factor. Thromb. Haemost. 67: 594-599
2. Ruggeri Z. M. and Zimmerman T. S. (1981) The complex multimeric composition of factor VIII von Willebrand factor. Blood 57: 1140-1143
3. Ruggeri Z. M. and Ware J. (1993) Von Willebrand factor. FASEB J. 7: 308-316
4. Mannucci P. M., Abildgaard C. F., Gralnick H. R., Hill F. G. H., Hoyer L. W., Lombardi R. et al. (1985) Multicenter comparison of von Willebrand factor multimer sizing techniques. Thromb. Haemost. 54: 873-877
5. Lynch D. C. (1993) The fine structure of von Willebrand factor multimers and type IIa von Willebrands disease. Ann. N. Y. Acad. Sci. 138: 138-152
6. Pannekoek H. and Voorberg H. (1989) Molecular cloning, expression and assembly of multimeric von Willebrand factor. Baillier's Clin. Haematol. 2: 879-896
7. Scott J. P. and Montgomery R. R. (1993) Therapy of von Willebrand disease. Sem. Thromb. Hemost. 19: 37-47
8. Bonthron D. T., Handin R. I., Kaufman R. J., Wasley L. C., Orr E. C., Mitsock L. M. et al. (1986) Structure of pre-provon Willebrand factor and its expression in heterologous cells. Nature 324: 270-273
9. Sixma J. J., Schiphorst M. E., Verwij C. L. and Pannekoek H. (1991) Effects of deletion of the A1 domain of von Willebrand factor on its binding to heparin, collagen and platelets in the presence of ristocetin. Eur. J. Biochem. 196: 369-375
10. Cruz M. A., Yuan H., Lee J. R., Wise R. J. and Handin R. I. (1995) Interaction of the von Willebrand factor (vWF) with collagen. J. Biol. Chem. 270: 10822-10827
11. Ribba A. A., Voorberg J., Meyer D., Pannekoek H. and Pieto G. (1992) Characterization of recombinant von Willebrand factor corresponding to mutations in type IIA and type IIB von Willebrand disease. J. Biol. Chem. 267: 23209-23215
12. Wise R. J., Pittman D. D., Handin R. I., Kaufman R. J. and Orkin S. H. (1988) The propeptide of von Willebrand factor independently mediates the assembly of von Willebrand multimers. Cell 52: 229-236
13. Verweij C. L., Hart M. and Pannekoek H. (1987) Expression of variant von Willebrand factor (vWf) cDNA in heterologous cells: requirement of the pro-polypeptide in vWf multimer formation. EMBO J. 6: 2885-2890
14. Meulien P., Nishino M., Mazurier C., Dott K., Pietu G., Jorieux S. et al. (1992) Processing and characterization of recombinant von Willebrand factor expressed in different cell types using a vaccinia virus vector. Thromb. Haemost. 67: 154-160
15. Mayadas T. N. and Wagner D. D. (1992) Vicinal cysteines in the prosequence play a role in von Willebrand factor multimer assembly. Prox. Natl. Acad. Sci. USA 89: 3531-3535
16. Kaufman R. J., Wasley L. C., Davies M. V., Wise R. J., Israel D. I. and Dorner A. J. (1989) Effect of von Willebrand factor coexpression on the synthesis and secretion of factor VIII in chinese hamster ovary cells. Mol. Cell. Biol. 9: 1233-1242
17. Voorberg J., Frontijn R., Calafat J., Janssen H., van Mourik J. A. and Pannekoek H. (1993) Biogenesis of von Willebrand factor-containing organelles in heterologous transfected CV-1 cells. EMBO J. 12: 749-758
18. Randi A. M., Joricux S., Tuley E. A., Mazurier C. and Sadler J. E. (1992) Recombinant von Willebrand factor Arg578 → Gln. J. Biol. Chem. 267: 21187-21192
19. Wise R. J., Dorner A. J., Krane M., Pittman D. D. and Kaufman R. (1991) The role of von Willebrand factor multimers and propeptide cleavage in binding and stabilization of factor ViII. J. Biol. Chem. 266: 21948-21955
20. Matsushita T. and Sadler J. E. (1995) Identification of amino acid residues essential for von Willebrand factor binding to platelet glycoprotein Ib. J. Biol. Chem. 270: 13406-13414
21. Lankhof H., Wu Y. P., Vink T., Schiphorst M. T., Zerwes H. G., de Groot P. G. and Sixma J. J. (1995) Role of the glycoprotein Ib-binding A1 repeat and the RGD sequence in platelet adhesion to human recombinant von Willebrand factor. Blood 86: 1035-1042
22. Carew J. A., Quinn S. M., Stoddart J. H. and Lynch D. C. (1992) O-linked carbohydrate of recombinant von Willebrand factor influences ristocetin-induced binding to platelet glycoprotein Ib. J. Clin. Invest. 90: 2258-2267
23. Rabinowit- I., Randi A. M., Shindler K. S., Tuley E. A., Rustagi P. K. and Sadler J. E. (1993) Type IIB mutation His-505-Asp implicates a new segment in the control of von Willebrand factor binding to platelet glycoprotein Ib. J. Biol. Chem. 268: 20497-20501
24. Jorieux S., Tuley E. A., Gaucher C., Mazurier C. and Sadler J. E. (1992) The mutation Arg (53)→Trp causes von Willebrand disease Normandy by abolishing binding to factor VIII: studies with recombinant von willebrand factor. Blood 79: 563-567
25. Azuma H., Sugimoto M., Ruggeri Z. M. and Ware J. (1993) A role for von Willebrand factor proline residues 702-704 in ristocetin-mediated binding to platelet glycoprotein Ib. Thromb. Haemost. 69: 192-196
26. Azuma H., Dent J. A., Sugimoto M., Ruggeri Z. M. and Ware J. (1991) Independent assembly and secretion of a dimeric adhesive domain of von Willebrand factor containing the glycoprotein Ib-binding site. J. Biol. Chem. 266: 12342-12347
27. Hilbert E., Gaucher C. de Romeuf C., Horellou M.-H., Vink T. and Mazurier C. (1994) Leu 697 → Val mutation in mature von Willebrand factor is responsible for type IIB von Willebrand disease. Blood 83: 1542-1550
28. Fischer B., Mitterer A., Schlokat U., DenBouwmeester R. and Dorner F. (1994) Structural analysis of recombinant von Willebrand factor: identification of hetero- and homo-dimers. FEBS Lett. 351: 345-348
29. Fischer B. E., Schlokat U., Mitterer M., Reiter M., Mundt W., Turecek P. L. et al. (1995) Structural analysis of recombinant von Willebrand factor produced at industrial scale fermentation of transformed CHO cells co-expressing recombinant furin. FEBS Lett. 375: 259-262
30. Schlokat U., Fischer B. E., Herlitschka S., Antoine G., Preiniger A., Mohr G. et al. (1996) Production of highly homologous and structurally intact recombinant von Willebrand factor multimers for furin-mediated propeptide removal in vitro. Biotechnol. Appl. Biochem. 24: 257-267
31. Schlokat U., Fischer B., Mitterer A., Falkner F. G., Reiter M., Grillberger L. et al. (1996) Herstellung and Charakterisierung von rekombinanter von Willebrand Faktor zur therapeutischen Anwendung. In: 26. Hämophilie-Symposium Hamburn 1995, pp. 147-158, Scharrer I. and Schramm W. (eds), Springer Verlag, Berlin
32. Turecek P. L., Siekmann J., Pichler L., Fischer B. E., Schlokat U., Metterer A. et al. (1996) Rekombinanter von Willebrand-Faktor: Präklinik. In: 26. Hämophilie-Symposium Hamburn 1995, pp. 159-168, Scharrer I. and Schramm W. (eds). Springer Verlag, Berlin
33. Schwarz H. P., Auer W., Bonneau M., Dorner F., Drouet L., Falkner F. et al. (1996) The effect of recombinant human von Willebrand factor in animal models of severe von Willebrand disease. Haemophilia 2 (Suppl. 1): 95
34. Drouet L., Roussi J., Bonneau M., Pignaud G. A., Turecek P. L., Dorner F. et al. (1995) The effect of recombinant human von Willebrand factor in pigs with severe von Willebrand's disease. Blood 86 (Suppl. 1): 612
35. Turecek P. L., Gritsch H., Pichler L., Auer W., Fischer B., Mitterer A. et al. (1997) In vivo charaeterization of recombinanl von Willebrand factor in dogs with von Willebrand disease. Blood 90: 3555-3567
36. Siekmann J., Turecek P. L., Fischer B. F., Mitterer A., Schlokat V., Falkner F. et al. (1995) Characterization of plasma-derived and recombinant human vWF by improved collagen binding assays. Thromb. Haemost 73: 1160
37. O'Shannessy D. J., Bringham-Burke M., Soneson K. K., Hensley P. and Brooks I. (1993) Determination of rate and equilibrium binding constants for macromolecular interactions using surface plasmon resonance: use of nonlinear least squares analysis methods. Anal. Biochem. 212: 457-468
38. Karlsson R. (1994) Real-time competitive kinetic analysis of interactions between low-molecular-weight ligands in solution and surface-immobilized receptors. Anal. Biochem. 221: 142-151
39. Malmqvist M. (1993) Biospecific interaction analysis using biosensor technology. Nature 361: 186-187
40. Fischer B., Mitterer A., Dorner F. and Eibl J. (1996) Comparison of N-glycan pattern of recombinant human coagulation factors II and IX expressed in Chinese hamster ovary (CHO) and African green monkey (Vero) cells. J. Thrombosis Thrombolysis 3: 57-62
41. Fujimura Y. and Titani K. (1994) Structure and function of von Willebrand factor. In: Haemostasis and Thrombosis, pp. 379-395, Bloom A. L., Forbes C. D., Thomas D. P. and Tuddenham E. G. D. (eds). Churchill Livingstone, Edinburgh
42. Mannucci P. M., Lattuada A. and Ruggeri Z. M. (1994) Proteolysis of von Willebrand factor in therapeutic plasma concentrates. Blood 83: 3018-3027
43. De Romeuf C. and Mazurier C. (1993) Heparin binding assay of von Willebrand factor (vWF) in plasma milieu: evidence of the importance of the multimerization degree of vWF. Thromb. Haemost. 69: 436-440
44. Vlot A. J., Koppelman S. J. van den Berg M. H., Bouma B. N. and Sixma J. J. (1995) The affinity and stoichiometry of binding of human factor VIII to von Willebrand factor. Blood 85: 3150-3157
45. Leyte A., Verbeet M. Ph., Brodniewicz-Proba T., Van Mourik J. and Mertens K. (1989) The interaction between human blood-coagulation factor VIII and von Willebrand factor: characterization of a high-affinity binding site on factor VIII. Biochem. J. 257: 679-683