Evaluation of an alanine racemase gene as an indicator for the detection of various Escherichia coli: Reactivity of the gene fragment with various E. coli O157:H7 isolates

Journal of Fermentation and Bioengineering

Volumn 84, Issue 5, 1997, Pages 471-474

  Yokoigawa, Kumio ^a   Okubo, Yoko ^a   Nakahira, Mayumi ^a   Umesako, Seiichi ^b   Imai, Shunsuke ^b   Kawai, Hiroyasu ^a  

^a NARA WOMEN'S UNIVERSITY   (Japan)

^b Nara Prefectural Institute of Public Health   (Japan)

Author keywords

Alanine racemase; Escherichia coli O157:H7; Hybridization

Indexed keywords

ALANINE RACEMASE; VEROTOXIN;

ARTICLE; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATION; DNA HYBRIDIZATION; ESCHERICHIA COLI; GENETIC ANALYSIS; NONHUMAN; BACTERIUM CONTAMINATION; BACTERIUM DETECTION; CONTROLLED STUDY; DNA DETERMINATION; GENE AMPLIFICATION; POLYMERASE CHAIN REACTION; PULSED FIELD GEL ELECTROPHORESIS; SEROTYPING;

CONTAMINATION; DNA; ENZYMES; PROBES;

ALANINE RACEMASE GENE; ESCHERICHIA COLI; HYBRIDIZATION; VEROCYTOTOXIN;

GENES;

EID: 0031444918     PISSN: 0922338X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0922-338X(97)82010-7     Document Type: Article

References (15)

1. Food and Drug Administration: Coliform bacteria. Bacteriological Analytical Manual, 6th ed., Chapter 5 (1984).
2. Hsu, H.-Y., Chan, S. W., Sobell, D. I., Halbert, D. N., and Groody, E. P.: A colorimetric DNA hybridization method for the detection of Escherichia coli in foods. J. Food Protect., 54, 249-255 (1991).
3. Okrend, A. J. G., Rose, B. E., and Bennett, B. J.: A screening method for the isolation of Escherichia coli O157:H7 from ground beef. Food Protect., 53, 249-252 (1990).
4. Ratnam, S., March, S. B., Ahmed, R., Bezanson, G. S., and Kasatiya, S.: Characterization of Escherichia coli serotype O157:H7. J. Clin. Microbiol., 26, 2006-2012 (1988).
5. Yokoigawa, K., Tomioka, R., Okubo, Y., and Kawai, H.: An alanine racemase gene as a new index for detecting Escherichia coli in foods. Biosci. Biotech. Biochem., 60, 1799-1804 (1996).
6. Heuvelink, A. E., Van de Kar, N. C., Meis, J. F., Monnens, L. A., and Meichers, W. J.: Characterization of verocytotoxin-producing Escherichia coli O157 isolates from patients with haemolytic uremic syndrome in western Europe. Epidemiol. Infect., 115, 1-14 (1995).
7. Meng, J., Zhao, S., Zhao, T., and Doyle, M. P.: Molecular characterization of Escherichia coli O157:H7 isolates by pulsed-field gel electrophoresis and plasmid DNA analysis. J. Med. Microbiol., 42, 258-263 (1995).
8. Ostroff, S. M., Tarr, P. I., Neill, M. A., Lewis, J. H., Bean, N. H., and Kobayashi, J. M.: Toxin genotypes and plasmid profiles as determinants of systemic sequelae in Escherichia coli O157:H7 infections. J. Infect. Dis., 160, 994-998 (1989).
9. Whittam, T. S., Wacbsmuth, I. K., and Wilson, R. A.: Genetic evidence of clonal descent of Escherichia coli O157:H7 associated with hemorrhagic colitis and hemolytic uremic syndrome. J. Infect. Dis., 157, 1124-1133 (1988).
10. Swerdlow, D. L., Woodruff, B. A., Brady, R. C., Griffin, P. M., Tippen, S., Donnell, H. D., Geldreich, E., Payne, B. J., Meyer, A., Wells, J. G., Greene, K. D., Bright, M., Bean, N. H., and Blake, P. A.: A waterborne outbreak in Missouri of Escherichia coli O157:H7 associated with bloody diarrhea and death. Ann. Intern. Med., 117, 812-818 (1992).
11. Ahmed, R., Bopp, C., Borczyk, A., and Kasatiya, S.: Phagetyping scheme for Escherichia coli serotype O157:H7. J. Infect. Dis., 155, 806-809 (1987).
12. Selander, R. K. and Levin, B. R.: Genetic diversity and structure in Escherichia coli populations. Science, 210, 545-547 (1980).
13. March, S. B. and Ratnam, S.: Sorbitol-MacConkey medium for detection of Escherichia coli O157:H7 associated with hemorrhagic colitis. J. Clin. Microbiol., 23, 869-872 (1986).
14. Nelson, K. and Selander, R. K.: Evolutionary genetics of the proline permease gene (putP) and the control region of the proline utilization operon in populations of Salmonella and Escherichia coli. J. Bacteriol., 174, 6886-6895 (1992).
15. Nelson, K., Whittam, T. S., and Selander, R. K.: Nucleotide polymorphism and evolution in the glyceraldehyde-3-phosphate dehydrogenase gene (gapA) in natural populations of Salmonella and Escherichia coli. Proc. Natl. Acad. Sci. USA, 88, 6667-6671 (1991).