Freeze-fracture enzyme cytochemistry reveals the distribution of enzymes in biological membranes: Enzyme cytochemical label-fracture and fracture-label

Acta Histochemica et Cytochemica

Volumn 30, Issue 1, 1997, Pages 77-84

  Takizawa, Toshihiro ^a   Nakazawa, Eiko ^b   Saito, Takuma ^a  

^a JICHI MEDICAL UNIVERSITY   (Japan)

^b HITACHI LTD   (Japan)

Author keywords

Enzyme cytochemistry; Fracture label; Freeze fracture cytochemistry; Freeze fracture electron microscopy; Label fracture

Indexed keywords

EID: 0031433232     PISSN: 00445991     EISSN: None     Source Type: Journal    
DOI: 10.1267/ahc.30.77     Document Type: Article

References (20)

1. Andersson Forsman, C. and Pinto da Silva, P.: Fracture-flip: new high-resolution images of cell surfaces after carbon stabilization of freeze-fractured membranes. J. Cell Sci. 90; 531-541, 1988.
2. DePierre, J. W. and Karnovsky, M. L.: Ecto-enzymes of the guinea pig polymorphonuclear leukocyte. I. Evidence for an ecto-adenosine monophosphatase, -adenosine triphosphatase, and -p-nitrophenyl phosphates. J. Biol. Chem. 249; 7111-7120, 1974.
3. Elias, P. M., Friend, D. S. and Goerke, J.: Membrane sterol heterogeneity: Freeze-fracture detection with saponins and filipin. J. Histochem. Cytochem. 27; 1247-1260, 1979.
4. Fisher, K. A. and Branton, D.: Freeze-fracture autoradiography: feasibility. J. Cell Biol. 70; 453-458, 1976.
5. Fujimoto, K.: Freeze-fracture replica electron microscopy combined with SDS digestion for cytochemical labeling of integral membrane proteins: Application to the immunogold labeling of intercellular junctional complexes. J. Cell Sci. 108; 3443-3449, 1995.
6. Fujimoto, K. and Pinto da Silva, P.: Surface views of nuclear pores in isolated rat liver nuclei as revealed by fracture-flip/Triton-X. Eur. J. Cell Biol. 50; 390-397, 1989.
7. Fujimoto, T. and Ogawa, K.: Cytochemical studies on the intramembranous particles of erythrocyte membranes. Acta Histochem. Cytochem. 13; 72-89, 1980.
8. Ohno, S. and Fujii, Y.: Three-dimensional and histochemical studies of peroxisomes in cultured hepatocytes by quick-freezing and deep-etching method. Histochemical J. 22; 143-154, 1990.
9. Pinto da Silva, P.: Freeze-fracture cytochemistry. In "Immunolabelling for Electron Microscopy", eds. by J. M. Polak and I. M. Varndell, Elsevier, Amsterdam, 1984, pp. 179-188.
10. Pinto da Silva, P.: Topology, dynamics, and molecular cytochemistry of integral membrane proteins: A freeze-fracture view. In "Electron Microscopy of Proteins", vol. 6, eds. by J. R. Harris and R. W. Horne, Academic Press, London, 1987, pp. 1-38.
11. Rix, E., Schiller, A. and Taugner, R.: Freeze-fracture-autoradiography. Histochemistry 50; 91-101, 1976.
12. Robinson, J. M. and Karnovsky, M. J.: Ultrastructural localization of 5′-nucleotidase in guinea pig neutrophils based upon the use of cerium as capturing agent. J. Histochem. Cytochem. 31; 1190-1196, 1983.
13. Robinson, J. M. and Karnovsky, M. J.: Ultrastructural localization of several phosphatases with cerium. J. Histochem. Cytochem. 31; 1197-1208, 1983.
14. Robinson, J. M., Karnovsky, M. L. and Karnovsky, M. J.: Glycogen accumulation in polymorphonuclear leukocytes, and other intracellular alternations that occur during inflammation. J. Cell Biol. 95; 933-942, 1982.
15. Ru-Long, S. and Pinto da Silva, P.: Simulcast: contiguous views of fracture faces and membrane surfaces in a single cell. Eur. J. Cell Biol. 53; 122-130, 1990.
16. Takizawa, T. and Robinson, J. M.: Combined immunocytochemistry and enzyme cytochemistry on ultra-thin cryosections: A new method. J. Histochem. Cytochem. 41; 1635-1639, 1993.
17. Takizawa, T. and Saito, T.: Freeze-fracture enzyme cytochemistry: Application of enzyme cytochemistry to freeze-fracture cytochemistry. J. Electron Microsc. 45; 242-246, 1996.
18. Takizawa, T. and Saito, T.: New fracture-labelling method: Alkaline phosphatase in unstimulated human neutrophils. J. Electron Microsc. 46; 85-92, 1997.
19. Verkleij, A. J., Kruijff, B. de, Gerritsen, W. F., Demel, R. A., Deenen, L. L. van and Ververgaert, P. H.: Freeze-etch electron microscopy of erythrocytes, Acholeplasma laidlawii cells and liposomal membranes after the action of filipin and amphotericin B. Biochim. Biophys. Acta 291; 577-581, 1973.
20. Wachstein, M. and Meisel, E.: Histochemistry of hepatic phosphatases at a physiologic pH. Am. J. Clin. Pathol. 27; 13-23, 1957.