Freeze-fracture enzyme cytochemistry: Application of enzyme cytochemistry to freeze-fracture cytochemistry

Journal of Electron Microscopy

Volumn 45, Issue 3, 1996, Pages 242-246

  Takizawa, Toshihiro ^a   Saito, Takuma ^a  

^a JICHI MEDICAL UNIVERSITY   (Japan)

Author keywords

Acid phosphatase; Cerium; Enzyme cytochemistry; Freeze fracture cytochemistry; Lysosome

Indexed keywords

ACID PHOSPHATASE; CERIUM; ENZYME;

ANIMAL; ARTICLE; CYTOCHEMISTRY; ELECTRON MICROSCOPY; ENZYMOLOGY; FREEZE FRACTURE; KIDNEY; LYSOSOME; MALE; METABOLISM; METHODOLOGY; RAT; SPRAGUE DAWLEY RAT; ULTRASTRUCTURE;

ACID PHOSPHATASE; ANIMALS; CERIUM; ENZYMES; FREEZE FRACTURING; HISTOCYTOCHEMISTRY; KIDNEY; LYSOSOMES; MALE; MICROSCOPY, ELECTRON; RATS; RATS, SPRAGUE-DAWLEY;

BIOLOGICAL MEMBRANES; CERIUM COMPOUNDS; FRACTURE; PHOSPHATASES;

ACID PHOSPHATASE; CYTOCHEMICAL; ENZYME CYTOCHEMISTRY; FREEZE FRACTURE; FREEZE-FRACTURE CYTOCHEMISTRY; LYSOSOME; MODELLING SYSTEMS; RAT KIDNEYS; TUBULAR EPITHELIUM;

CYTOLOGY;

EID: 0030164625     PISSN: 00220744     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.jmicro.a023440     Document Type: Article

References (11)

1. Pinto da Silva P: 1. Topology, dynamics, and molecular cytochemistry of integral membrane proteins: A freeze-fracture view. In: Harris JR, Horne RW, eds. Electron microscopy of proteins, vol 6. London: Academic Press, 1-38 (1987)
2. Robinson JM, Karnovsky MJ: Ultrastructural localization of several phosphatases with cerium. J Histochem Cytochem 31: 1197-1208 (1983)
3. Gomori G: Acid phosphatase. In: Gomori D, ed. Microscopic histochemistry. Chicago: Univ Chicago Press, 189-193 (1952)
4. Fukushima O, Yamashita H, Sakai T, Saito T: Application of ultrathin cryo-sections to enzyme cytochemistry. Acta Histochem Cytochem 22: 131-137 (1989)
5. Andersson-Forsman C, Pinto da Silva P: Fracture-flip: New high-resolution images of cell surfaces after carbon stabilization of freeze-fractured membranes. J Cell Sci 90: 531-541 (1988)
6. Fujimoto K, Pinto da Silva P: Surface views of nuclear pores in isolated rat liver nuclei as revealed by fracture-flip/Triton-X. Eur J Cell Biol 50: 390-397 (1989)
7. Ru-Long S, Pinto da Silva P: Simulcast: Contiguous views of fracture faces and membrane surfaces in a single cell. Eur J Cell Biol 53: 122-130 (1990)
8. Fujimoto T, Ogawa K: Cytochemical studies on the intramembranous particles of erythrocyte membranes. Acta Histochem Cytochem 13: 72-89 (1980)
9. Coleman RA, Harris HW, Wade JB: Visualization of endocytosed markers in freeze-fracture studies of toad urinary bladder. J Histochem Cytochem 35: 1405-1414 (1987)
10. Ohno S, Fujii Y: Three-dimensional and histochemical studies of peroxisomes in cultured hepatocytes by quick-freezing and deep-etching method. Histochem J 22: 143-154 (1990)
11. ) Fujimoto K: Freeze-fracture replica electron microscopy combined with SDS digestion for cytochemical labeling of integral membrane proteins: Application to the immunogold labeling of intracellular junctional complexes. J Cell Sci 108: 3443-3449 (1995)