A synthetic peptide corresponding to the putative dihydrofolate reductase domain of nitric oxide synthase inhibits uncoupled NADPH oxidation

Nitric Oxide - Biology and Chemistry

Volumn 1, Issue 1, 1997, Pages 50-55

  Mayer, Bernd ^a   Pitters, Eva ^a   Pfeiffer, Silvia ^a   Kukovetz, Walter R ^a   Schmidt, Kurt ^a  

^a UNIVERSITY OF GRAZ   (Austria)

Author keywords

[No Author keywords available]

Indexed keywords

ARGININE; BIOPTERIN; CITRULLINE; CYTOCHROME C; DIHYDROFOLATE REDUCTASE; N(G) NITROARGININE; NITRIC OXIDE SYNTHASE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; SYNTHETIC PEPTIDE;

ARTICLE; CATALYSIS; ELECTRON TRANSPORT; ENZYME BINDING; INSECT; OXIDATION; PRIORITY JOURNAL;

INSECTA; UNIDENTIFIED BACULOVIRUS;

EID: 0031431098     PISSN: 10898603     EISSN: None     Source Type: Journal    
DOI: 10.1006/niox.1996.0102     Document Type: Article

References (29)

1. Griffith O. W., Stuehr D. J. Nitric oxides synthases: Properties and catalytic mechanism. Annu. Rev. Physiol. 57:1995;707-736.
2. Förstermann U., Kleinert H. Nitric oxide synthase: expression and expressional control of the three isoforms. Naunyn-Schmiedeberg's Arch. Pharmacol. 352:1995;351-364.
3. Mayer B. Biochemistry and molecular pharmacology of nitric oxide synthases. In. 1995;Academic Press, San Diego. p. 21-42.
4. Fulco A. J. P450BM-3 and other inducible bacterial P450 cytochromes: Biochemistry and regulation. Annu. Rev. Pharmacol. Toxicol. 31:1991;177-203.
5. Mayer B., Werner E. R. In search of a function for tetrahydrobiopterin in the biosynthesis of nitric oxide. Naunyn-Schmiedeberg's Arch. Pharmacol. 351:1995;453-463.
6. Nishimura J. S., Martasek P., McMillan K., Salerno J. C., Liu Q., Gross S. S., Masters B. S. S. Modular structure of neuronal nitric oxide synthase: Localization of the arginine binding site and modulation by pterin. Biochem. Biophys. Res. Commun. 210:1995;288-294.
7. McMillan K., Masters B. S. S. Prokaryotic expression of the heme- and flavin-binding domains of rat neuronal nitric oxide synthase as distinct polypeptides: Identification of the heme-binding proximal thiolate ligand as cysteine-415. Biochemistry. 34:1995;3686-3693.
8. Wang J. L., Stuehr D. J., Rousseau D. L. Tetrahydrobiopterin-deficient nitric oxide synthase has a modified heme environment and forms a cytochrome P-420 analogue. Biochemistry. 34:1995;7080-7087.
9. Klatt P., Pfeiffer S., List B. M., Lehner D., Glatter O., Werner E. R., Schmidt K., Mayer B. Characterization of heme-deficient neuronal nitric oxide synthase reveals role for heme in subunit dimerization and binding of amino acid substrate and tetrahydrobiopterin. J. Biol. Chem. 271:1996;7336-7342.
10. Chen P. F., Tsai A. L., Wu K. K. Cysteine-99 of endothelial nitric oxide synthase (NOS-III) is critical for tetrahydrobiopterin-dependent NOS-III stability and activity. Biochem. Biophys. Res. Commun. 215:1995;1119-1129.
11. D. K. Gosh, C. Wu, B. Mayer, D. J. Stuehr, 1996, Identification of a 53 aa region in NO synthase involved in tetrahydrobiopterin binding and subunit dimerization, In, Los Angeles, CA.
12. Cho H. J., Martin E., Xie Q. W., Sassa S., Nathan C. Inducible nitric oxide synthase: Identification of amino acid residues essential for dimerization and binding of tetrahydrobiopterin. Proc. Natl. Acad. Sci. USA. 92:1995;11514-11518.
13. Harteneck C., Klatt P., Schmidt K., Mayer B. Expression of rat brain nitric oxide synthase in baculovirus-infected insect cells and characterization of the purified enzyme. Biochem. J. 304:1994;683-686.
14. Mayer B., List B. M., Klatt P., Harteneck C., Schmidt K. Large scale purification of rat brain nitric oxide synthase from a baculovirus overexpression system. Methods Enzymol. 268:1996;420-427.
15. Hinsch K.-D., Tychowiecka I., Gausepohl H., Frank R., Rosenthal W., Schultz G. Tissue distribution of β1- and β2-subunits of regulatory guanine nucleotide-binding proteins. Biochim. Biophys. Acta. 1013:1989;60-67.
16. Guthmann F., Mayer B., Koesling D., Kukovetz W. R., Böhme E. Characterization of soluble platelet guanylyl cyclase with peptide antibodies. Naunyn-Schmiedeberg's Arch. Pharmacol. 346:1992;537-541.
17. Mayer B., Klatt P., Werner E. R., Schmidt K. Molecular mechanism of inhibition of porcine brain nitric oxide synthase by the antinociceptive drug 7-nitroindazole. Neuropharmacology. 33:1994;1253-1259.
18. 2+. J. Biol. Chem. 267:1992;11374-11378.
19. Klatt P., Schmidt K., Brunner F., Mayer B. Inhibitors of brain nitric oxide synthase: Binding kinetics, metabolism, and enzyme inactivation. J. Biol. Chem. 269:1994;1674-1680.
20. Klatt P., Schmid M., Leopold E., Schmidt K., Werner E. R., Mayer B. The pteridine binding site of brain nitric oxide synthase-tetrahydrobiopterin binding kinetics, specificity, and allosteric interaction with the substrate domain. J. Biol. Chem. 269:1994;13861-13866.
21. Edwards R. J., Singleton A. M., Murray B. P., Sesardic D., Rich K. J., Davies D. S., Boobis A. R. An anti-peptide antibody targeted to a specific region of rat cytochrome P-450/A2 inhibits enzyme activity. Biochem. J. 266:1990;497-504.
22. 2+. J. Biol. Chem. 265:1990;11091-11097.
23. Foster C. J., Johnston S. A., Sunday B., Gaet F. C. Potent peptide inhibitors of smooth muscle myosin light chain kinase: Mapping of the pseudosubstrate and calmodulin binding domains. Arch. Biochem. Biophys. 280:1990;397-404.
24. Omata Y., Sakamoto H., Robinson R. C., Pincus M. R., Friedman F. K. Interaction between cytochrome P450 2B1 and cytochrome b5: Inhibition by synthetic peptides indicates a role for P450 residues Lys-122 and Arg-125. Biochem. Biophys. Res. Commun. 201:1994;1090-1095.
25. Shen S., Strobel H. W. Probing the putative cytochrome P450- and cytochrome c-binding sites on NADPH-cytochrome P450 reductase by antipeptide antibodies. Biochemistry. 33:1994;8807-8812.
26. J. C. Salerno, D. E. Harris, K. Irizzary, S. M. E. Smith, K. McMillan, P. Martasek, L. J. Roman, B. B. S. Masters, C. Jones, B. A. Weissman, Q. Liu, S. S. Gross, 1996, The inhibitory polypeptide of constitutive NOS (cNOS) is the missing control site element of the inducible isoform (iNOS), In, Los Angeles, CA.
27. Mayer B., John M., Heinzel B., Werner E. R., Wachter H., Schultz G., Böhme E. Brain nitric oxide synthase is a biopterin- and flavin-containing multi-functional oxido-reductase. FEBS Lett. 288:1991;187-191.
28. 2+. Biochem. J. 281:1992;627-630.
29. Pou S., Pou W. S., Bredt D. S., Snyder S. H., Rosen G. M. Generation of superoxide by purified brain nitric oxide synthase. J. Biol. Chem. 267:1992;24173-24176.