The biosynthesis of docosahexaenoic acid [122:6(n-3)] from linolenic acid in primary hepatocytes isolated from wild northern pike

Journal of Fish Biology

Volumn 51, Issue 6, 1997, Pages 1197-1208

  Buzzi, M ^a   Henderson, R J ^a   Sargent, J R ^a  

^a UNIVERSITY OF STIRLING   (United Kingdom)

Author keywords

Biosynthesis; Desaturation; Docosahexaenoic acid; Elongation; Esox lucius; Hepatocyte; Pike

Indexed keywords

EID: 0031426652     PISSN: 00221112     EISSN: None     Source Type: Journal    
DOI: 10.1006/jfbi.1997.0519     Document Type: Article

References (28)

1. Baksi S. M., Frazier J. M. Isolated fish hepatocytes: model systems for toxicology research. Aquatic Toxicology. 16:1990;229-256.
2. Buzzi M., Henderson R. J., Sargent J. R. The desaturation and elongation of linolenic acid and eicosapentaenoic acid by hepatocytes and liver microsomes from rainbow trout (Oncorhynchus mykiss. Biochimica Biophysica Acta. 1299:1996;235-244.
3. Buzzi M., Henderson R. J., Sargent J. R. The biosynthesis of docosahexaenoic acid in trout hepatocytes proceeds via 24-carbon intermediates. Comparative Biochemistry and Physiology. 116B:1997;263-267.
4. Christie W. W. The isolation of lipids from tissues. Lipid Analysis. 1982a;Pergamon Press, Oxford.
5. Christie W. W. The preparation of derivatives of lipids. Christie W. W. Lipid Analysis. 1982b;Pergamon Press, Oxford.
6. Desvilettes C., Bourdier G., Breton J.-C. Lipid class and fatty acid composition of planktivorous larval pikeEsox lucius. Aquatic Living Resources. 7:1994;67-77.
7. Farkas T., Csengeri I. Biosynthesis of fatty acids by the carpCyprinus carpio. Lipids. 11:1976;401-407.
8. Ghioni C., Bell G. B., Sargent J. R. Polyunsaturated fatty acids in neutral lipids and phospholipids of some freshwater insects. Comparative Biochemistry and Physiology. 114B:1996;161-170.
9. Gurr M. I., Harwood J. L. Lipid Biochemistry. 1991;Chapman & Hall, London.
10. Henderson R. J., Tocher D. R. Thin-layer chromatography. Hamilton J. R., Hamilton S. Lipid Analysis. 1992;IRL Press, New York.
11. 14Esox lucius. Fish Physiology and Biochemistry. 144:1995;223-235.
12. Kanazawa A., Teshima S.-I., Ono K. Relationship between essential fatty acid requirements of aquatic animals and the capacity for bioconversion of linolenic acid to highly unsaturated fatty acids. Comparative Biochemistry and Physiology. 63B:1979;295-298.
13. Klaunig J. E., Ruch R. L., Goldblatt P. J. Trout hepatocyte culture: Isolation and primary culture. In Vitro. 21:1985;221-228.
14. 14. Comparative Biochemistry and Physiology. 48B:1974;641-649.
15. 14Scophthalmus maximus. Journal of Fish Biology. 38:1991;335-347.
16. Linko R. R., Karinkanta H. Fatty acid of long chain length in baltic herring lipids. Journal of the American Oil Chemists Society. 47:1970;42-46.
17. Moon T. W., Walsh P. J., Mommsen T. P. Fish hepatocytes, a model metabolic system. Canadian Journal of Fisheries and Aquatic Science. 42:1985;1772-1782.
18. 14Liza auratain vivo. Fish Physiology and Biochemistry. 12:1993;119-130.
19. 1414. Biochimica et Biophysica Acta. 1212:1994;109-118.
20. Olsen R. E., Henderson J. R., McAndrew B. J. The conversion of linoleic acid and linolenic acid to longer chain polyunsaturated fatty acids byTilapia (Oreochromis) nilotica in vivo. Fish Physiology and Biochemistry. 8:1990;261-270.
21. Ota T., Chihara Y., Itabashi Y., Takagi T. Occurrence of all-cis-6,9,12,15,18,21-tetracosahexaenoic acid in flatfish lipids. Fisheries Science. 60:1994a;171-175.
22. Ota T., Kawabata T., Ando Y. Positional distribution of 24:6(n-3) in triacyl-sn-glycerols from flathead flounder liver and flesh. Journal of the American Oil Chemists Society. 71:1994b;475-478.
23. Owen J. M., Adron J. W., Middleton C., Cowey C. B. Elongation and desaturation of dietary fatty acids in turbotScophthalmus maximusSalmo gairdneri. Lipids. 10:1975;528-531.
24. Sargent J. R., Bell M. V., Henderson R. J., Tocher D. R. Polyunsaturated fatty acids in marine and terrestrial food webs. Journal of Comparative Physiology. 5:1990;11-23.
25. Schwalme K. A quantitative comparison between diet and body fatty acid composition in wild northern pike (Esox lucius. Fish Physiology and Biochemistry. 10:1992;91-98.
26. Schwalme K., Mackay W. C., Clandinin M. T. Seasonal dynamics of fatty acid composition in female northern pike (Esox lucius. Journal of Comparative Physiology. 163B:1993;227-287.
27. Voss A., Reinhart M., Sankarappa S., Sprecher H. The metabolism of 7,10,13,16,19-docosapentaenoic acid to 4,7,10,13,16,19-docosahexaenoic acid in rat liver is independent of Δ4-desaturase. Journal of Biological Chemistry. 266:1991;19 995-20 000.
28. Wilson R., Sargent J. R. High-resolution separation of polyunsaturated fatty acids by argentation thin-layer chromatography. Journal of Chromatography. 623:1992;403-407.