p53 protein expression and increased SSCP mobility shifts in the p53 gene in normal urothelium cultured from smokers

Carcinogenesis

Volumn 18, Issue 6, 1997, Pages 1241-1245

  Mothersill, C ^c   O'Malley, K ^a   Colucci, S ^c   Murphy, D ^a   Lynch, T ^a   Payne, S ^b   Seymour, C ^c   Harney, J ^a,b  

^a BEAUMONT HOSPITAL   (Ireland)

^b MANCHESTER ROYAL INFIRMARY   (United Kingdom)

^c DUBLIN INSTITUTE OF TECHNOLOGY   (Ireland)

Author keywords

[No Author keywords available]

Indexed keywords

PROTEIN P53;

ADULT; AGED; ARTICLE; BLADDER BIOPSY; CONTROLLED STUDY; FEMALE; GEL MOBILITY SHIFT ASSAY; HUMAN; HUMAN TISSUE; MAJOR CLINICAL STUDY; MALE; PRIORITY JOURNAL; PROTEIN EXPRESSION; SINGLE STRAND CONFORMATION POLYMORPHISM; SMOKING; STATISTICAL ANALYSIS; TUMOR SUPPRESSOR GENE; UROTHELIUM;

ADULT; AGED; AGED, 80 AND OVER; BIOPSY; FEMALE; GENES, P53; HUMANS; IMMUNOHISTOCHEMISTRY; INDIVIDUALITY; MALE; MIDDLE AGED; POLYMERASE CHAIN REACTION; POLYMORPHISM, SINGLE-STRANDED CONFORMATIONAL; SMOKING; TUMOR SUPPRESSOR PROTEIN P53; URINARY BLADDER;

EID: 0031408204     PISSN: 01433334     EISSN: None     Source Type: Journal    
DOI: 10.1093/carcin/18.6.1241     Document Type: Article

References (24)

1. International Agency for Research on Cancer (IARC) (1986) Monographs on the Evaluation of the Carcinogenic Risk of Chemicals to Humans. International Agency for Research on Cancer, Lyon, vol. 38.
2. Augustine, A., Herbert, J.R., Kabat, G.C. and Wynder, E.R. (1988) Bladder cancer in relation to cigarette smoking. Cancer Res., 48, 4405-4408.
3. Momas, I., Daures, J.P., Festy, B., Bontoux, J. and Gremy, F. (1994) Bladder cancer and black tobacco cigarette smoking. Some results from a French case control study. Eur. J. Epidemiol., 10, 599-604.
4. Bartsch, H., Malaveille, C., Friesen, M., Kadlubar, F.F. and Vineis, P. (1993) Black (air cured) and blond (flue cured) tobacco cancer risk. IV: molecular dosimetry studies implicate aromatic amines as bladder carcinogens. Eur. J. Cancer, 29A, 1199-1207.
5. Hecht, S.S., Carmella, S.G., Murphy, S.E. et al. (1993) A tobacco specific lung carcinogen in the urine of men exposed to cigarette smoke. NEJM, 329, 1543-1546.
6. Mothersill, C., Seymour, C.B., Harney, J. and Hennessy, T.P. (1994) High levels of stable p53 protein and of cmyc in cultured human epithelial tissue after radiation. Radiat. Res., 137, 317-322.
7. Harney, J., Murphy, D., Jones, M. and Mothersill, C. (1995) p53 expression in urothelium from normal and tumour bearing patients. Brit. J. Cancer, 71, 25-29.
8. Harney, J., Seymour, C.B., Murphy, D. and Mothersill, C. (1995) Variation in the expression of p53, cmyc and bcl-2 oncoproteins in individual patient cultures of normal urothelium exposed to cobalt 60γ-rays and N-nitrosodiethanolamine. Cancer Epidemiol. Biomarkers Prev., 4, 617-625.
9. Anton-Culver, H., Lee-Feldstein, A. and Taylor, T.H. (1993) The association of bladder cancer risk with ethnicity, gender and smoking. AEP, 3, 429-433.
10. Cannon, J.V., Greaves, R. and Lane, D.P. (1990) Activating mutations in p53 produce a common conformational effect. A monoclonal specific for the mutant form. EMBO J., 9, 1595-1602.
11. Lambkin, H., Mothersill, C., Chin, D. et al. (1997) p53 immunohistochemistry in breast adenocarcinoma - a panel of antibodies compared with single strand conformation polymorphism. Am. J. Pathol. (In press).
12. Moll, U.M., Riou, G. and Levine, A.J. (1992) Two distinct mechanisms alter p53 in breast cancer: mutation and nuclear exclusion. Proc. Natl Acad. Sci. USA, 89, 7262-7266.
13. Moll, U.M., Ostermeyer, A.G., Haladay, R., Winkfield, B., Frazier, M. and Zambetti, G. (1996) Cytoplasmic sequestration of wild-type p53 protein impairs the Gl checkpoint after DNA damage. Mol. Cell Biol., 16, 1126-1137.
14. Moll, U.M., LaQuaglia, M., Benard, J. and Riou, G. (1995) Wild-type p53 protein undergoes cytoplasmic sequestration in undifferentiated neuroblastomas but not in differentiated tumours. Proc. Nail Acad. Sci. USA, 92, 4407-4411.
15. Curigliano, G., Zhang, Y.G., Wang, L.Y., Flamini, G., Alcini, A., Ratto, C., Giustacchini, M., Alcini, E., Cittadini, A. and Santella, R.M., (1996). Immunohistochemical quantitation of 4-aminobiphenyl-DNA adducts and p53 nuclear over-expression in T1 bladder cancer of smokers and non-smokers. Carcinogenesis, 17, 911-916.
16. Colucci, S., Mothersill, C., Harney, J., Gamble, S., Parsons, K. and Arrand, J. (1997) Induction of a high degree of clonal genetic instability in p53 at multiple sites in cultures of normal human urothelium exposed to low dose gamma radiation. Int. J. Radiat. Biol. (in press).
17. Lane, D.P. (1992) p53, Guardian of the genome. Nature, 358, 15-16.
18. Field, J.K., Spandidos, D.A., Malliri, A., Gosney, J.R., Yiagnisis, M. and Stell, P.M. (1993) Elevated p53 expression correlates with a history of heavy smoking in squamous cell carcinoma of the head and neck. Br. J. Cancer, 64, 573-577.
19. Brennan, J.A., Boyle, J.O., Koch, W.M., Goodman, S.N., Hruban, R.H., Eby, Y.J., Couch, M.J., Forastiere, A.A. and Sidransky, D. (1995) Association between cigarette smoking and mutation of the p53 gene in squamous-cell carcinoma of the head and neck. N. Engl J. Med., 11, 712-717.
20. Kim, M.S., Shin, K., Baek, J., Cherrick, H.M. and Park, N. (1993) HPV-16, tobacco-specific N-nitrosamine, and N-methyl-N′nitro-N-nitrosoguanidine in oral carcinogenesis. Cancer Res., 53, 4811-4816.
21. Westra, W.H., Offerhaus, G.J., Goodman, S.N., Slebos, R.J.C., Polak, M., Baas, I.O., Rodenhuiis, S. and Hruban, R.H. (1993) Overexpression of the p53 tumor supressor gene product in primary lung adenocarcinoma is associated with cigarette smoking. Am. J. Surg. Pathol., 17, 213-220.
22. Simons, A.M., Philips, D.H. and Coleman, D.V. (1993) Damage to DNA in cervical epithelium related to smoking tobacco. Br. Med. J., 306, 1444-1448.
23. Palmer, J.R., Rosenberg, L., Clarke, E.A., Stolley, P.D., Warshauer, M.E., Zauber, A.G. and Shapiro, S. (1991) Breast cancer and cigarette smoking: a hypothesis. Am. J. Epidemiol., 134, 1.
24. Bridges, B.A., Cole, J. and Green, M.J. (1989) Smoking, leukaemia and lymphocyte mutations. Leukaemia, 3, 846.