Polyketide Synthase Gene pksM from Aspergillus terreus Expressed during Growth Phase

Folia Microbiologica

Volumn 42, Issue 5, 1997, Pages 419-430

  Pazoutova S ^a   Linka, M ^a   Storkova S ^a   Schwab, H ^b  

^a INSTITUTE OF MICROBIOLOGY   (Czech Republic)

^b GRAZ UNIVERSITY OF TECHNOLOGY   (Austria)

Author keywords

[No Author keywords available]

Indexed keywords

ASPERGILLUS TERREUS; PENICILLIUM; PENICILLIUM GRISEOFULVUM;

3 OXOACYL (ACYL CARRIER PROTEIN) REDUCTASE; 3 OXOACYL ACYL CARRIER PROTEIN SYNTHASE; 3-OXOACYL-(ACYL-CARRIER-PROTEIN) REDUCTASE; 6 METHYLSALICYLIC ACID SYNTHETASE; 6-METHYLSALICYLIC ACID SYNTHETASE; ACYL CARRIER PROTEIN; ACYLTRANSFERASE; ALCOHOL DEHYDROGENASE; FUNGAL DNA; FUNGAL RNA; HYDROLYASE; LIGASE; MULTIENZYME COMPLEX; OXIDOREDUCTASE;

AMINO ACID SEQUENCE; ARTICLE; ASPERGILLUS; DNA PROBE; DNA SEQUENCE; FUNGAL GENE; GENE EXPRESSION; GENE LIBRARY; GENETIC TRANSCRIPTION; GENETICS; GROWTH, DEVELOPMENT AND AGING; INTRON; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; NORTHERN BLOTTING; NUCLEIC ACID HYBRIDIZATION; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PENICILLIUM; PLASMID; POLYMERASE CHAIN REACTION; RESTRICTION MAPPING; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY; START CODON;

3-OXOACYL-(ACYL-CARRIER-PROTEIN) SYNTHASE; ACYL CARRIER PROTEIN; ACYLTRANSFERASES; ALCOHOL OXIDOREDUCTASES; AMINO ACID SEQUENCE; ASPERGILLUS; BASE SEQUENCE; BLOTTING, NORTHERN; CLONING, MOLECULAR; CODON, INITIATOR; DNA PROBES; DNA, FUNGAL; GENE EXPRESSION; GENE LIBRARY; GENES, FUNGAL; HYDRO-LYASES; INTRONS; LIGASES; MOLECULAR SEQUENCE DATA; MULTIENZYME COMPLEXES; NUCLEIC ACID HYBRIDIZATION; OPEN READING FRAMES; OXIDOREDUCTASES; PENICILLIUM; PLASMIDS; POLYMERASE CHAIN REACTION; RESTRICTION MAPPING; RNA, FUNGAL; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS, DNA; SEQUENCE HOMOLOGY, AMINO ACID; TRANSCRIPTION, GENETIC;

EID: 0031299651     PISSN: 00155632     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF02826548     Document Type: Article

References (34)

1. ALBERTS A.W.: Discovery, biochemistry and biology of lovastatin. Am.J.Cardiol. 62, 10J-15J (1988).
2. BECK J., RIPKA S., SIEGNER A., SCHILTZ E., SCHWEIZER E.: The multifunctional 6-methylsalicylic acid synthase gene of P. patulum. Eur.J.Biochem. 192, 487-498 (1990).
3. BEDFORD D.J., SCHWEIZER E., HOPWOOD D., KHOSLA C.: Expression of a functional fungal polyketide synthase in the bacterium Str. coelicolor A3(2). J.Bacteriol. 177, 4544-4548 (1995).
4. BEVITT D.J., CORTES J., HAYDOCK S.F., LEADLAY P.F.: 6-Deoxyerythronolide-B synthase 2 from Saccharopolyspora erythraea. Eur.J.Biochem. 204, 39-49 (1992).
5. BINGLE L.E.H., LAZARUS C.M.: Direct Submission. Gen Bank Accession no. U89769 (1997).
6. CHANG P.K., CARY J.W., YU J., BHATNAGAR D., CLEVELAND T.E.: The A. parasiticus polyketide synthase gene pksA, a homolog of A. nidulans wA, is required for aflatoxin B1 biosynthesis. Mol.Gen.Genet. 248, 270-277 (1995).
7. CHEN Z-G., FUJII I., EBIZUKA Y., SANKAWA U.: Emodin-O-methyltransferase from A. terreus. Arch.Microbiol. 158, 29-34 (1992).
8. CHOMCZYNSKI P., SACCHI N.: Single-step method of RNA isolation by acid guanidium thiocyanate-phenol-chloroform extraction. Anal.Biochem. 162, 156-159 (1987).
9. DAVIS R., ALDRICH T.L., NGUYEN D.K., HENDRICKSON L.E., ROACH C., VINCI V.A., MCADA P.C.: Functional mapping of a polyketide synthase from A. terreus involved in lovastatin synthesis. 2nd Eur. Conf. Fungal Genetics, Lunteren (The Netherlands) 1994.
10. DEVEREUX J., HAEBERLI P., SMITHIES O.: A comprehensive set of sequence analysis programs for the VAX. Nucl.Acid Res. 12, 387-395 (1984).
11. DONADIO S., KATZ L.: Organization of the enzymatic domains in the multifunctional polyketide synthase involved in erythromycin formation in Saccharopolyspora erythraea. Gene 111, 51-60 (1992).
12. DONADIO S., STAVER M.J., MCALPINE J.B., SWANSON S.J., KATZ L.: Modular organization of genes required for complex polyketide biosynthesis. Science 252, 675-679 (1991).
13. DRETZEN G., BELLARD M., SASSONE-CORSI P., CHAMBON P.: A reliable method for the recovery of DNA fragments from agarose and acrylamide gels. Anal.Biochem. 112, 295-298 (1981).
14. FENG G.H., LEONARD T.J.: Characterization of the polyketide synthase gene pksL1 required for aflatoxin biosynthesis in A. parasiticus. J.Bacteriol. 177, 6246-6254 (1995).
15. FENG G.H., LEONARD T.J.: Cultural conditions differentially control transcriptional expression of the homologous genes for aflatoxin/sterigmatocystin biosynthesis in Aspergillus parasiticus and A. nidulans. Gen Bank Accession no. U52151 (1997).
16. FUJII I., ONO Y., TADA H., GOMI K., EBIZUKA Y., SANKAWA U.: Cloning of the polyketide synthase gene atX from Aspergillus terreus and its identification as the 6-methylsalicylic acid synthase gene by heterologous expression. Mol.Gen.Genet. 253, 1-10 (1996).
17. FIDEL S., DOONAN J.H., MORRIS N.R.: Aspergillus nidulans contains a single actin gene which has unique intron locations and encodes a gamma-actin. Gene 70, 283-293 (1988).
18. GLOVER D.M.: DNA Cloning. A Practical Approach, Vol 1. IRL Press, Oxford-Washington 1985.
19. GREENSPAN M.D., YUDKOWITZ J.B.: Mevinolinic acid biosynthesis by Aspergillus terreus and its relationship to fatty acid synthesis. J.Bacteriol. 162, 704-707 (1985).
20. GURR S.J., UNKLES S.E., KINGHORN J.R.: The structure and organization of nuclear genes of filamentous fungi pp. 93-139 in J.R. Kinghorn (Ed.): Gene Structure of Eukaryotic Microbes. IRL Press, Oxford-Washington (DC) 1987.
21. HOPWOOD D.A., KHOSLA C.: Genes for polyketide secondary metabolic pathways in microorganisms and plants, pp. 88-112 in Secondary Metabolites, Their Function and Evolution. Ciba Foundation Symposium 171. Wiley, Chichester 1992.
22. HOPWOOD D.A., SHERMAN D.H.: Molecular genetics of polyketides and its comparison to fatty acid biosynthesis. Ann.Rev. Genet. 24, 37-66 (1990).
23. KENT J., HEATLEY N.G.: Antibiotics from moulds. Nature 156, 295-296 (1945).
24. MAYORGA M.E., TIMBERLAKE W.E.: The developmentally regulated A. nidulans wA gene encodes a polypeptide homologous to polyketide and fatty acid synthases. Mol.Gen.Genet. 235, 205-212 (1992).
25. REEVES C., HENDRICKSON L., SOLIDAY C., DAVIS R., REIMANN J., NGUYEN D.-K., SCHIMMEL T., VINCI V.: Biosynthesis of lovastatin and other polyketides in Aspergillus terreus. 1st Eur. Conf. Fungal Genetics, Nottingham (England) 1992.
26. ROACH C., DAVIS R., ALDRICH T.L., HENDRICKSON L.E., NGUYEN D.K., VINCI V.A., MCADA P.C.: Cloning and characterization of a gene for a 269 kD protein involved in lovastatin biosynthesis. 2nd Eur. Conf. Fungal Genetics, Lunteren (The Netherlands) 1994.
27. SHEEHAN J.C., LAWSON W.B., GAUL R.J.: The structure of terreic acid. J.Am.Chem.Soc. 80, 5536-5538 (1958).
28. TAKANO Y., KUBO Y., SHIMIZU K., MISE K., OKUNO T., FURUSAWA I.: Structural analysis of PKS1, a polyketide synthase gene involved in melanin biosynthesis in C. lagenarium. Mol.Gen.Genet. 249, 162-167 (1995).
29. VINCI V.A., HOERNER T.D., COFFMAN A.D., SCHIMMEL T.G., DABORA R.L., KIRPEKAR A.C., RUBY C.L., STIEBER R.W.: Mutants of a lovastatin hyperproducing A. terreus deficient in the production of sulochrin. J.Ind.Microbiol. 8, 113-120 (1991).
30. WANG I.-K. REEVES C., GAUCHER G.M.: Isolation and sequencing of a genomic clone containing the 3′ terminus of the 6-methylsalicylic acid polyketide synthetase gene of P. urticae. Can.J.Microbiol. 37, 86-95 (1991).
31. YANG G., ROSE M.S., TURGEON B.G., YODER O.C.: A polyketide synthase is required for fungal virulence and production of the polyketide T-toxin. Plant Cell. 8, 2139-2150 (1996).
32. YU J.-H., LEONARD T.J.: Sterigmatocystin biosynthesis on A. nidulans requires a novel type I polyketide synthase. J.Bacteriol. 177, 4792-4800 (1995).
33. ZAMIR L.O.: The biosynthesis of patulin and penicillic acid, pp. 223-268 in P. Steyn (Ed.): The Biosynthesis of Mycotoxins. Academic Press, New York-London-Toronto-Sydney-San Francisco 1980.
34. ZARET K.S., SHERMAN F.: DNA sequence required for efficient transcription termination in yeast. Cell 28, 563-573 (1982).