Characterization of an 'orthogonal' suppressor tRNA derived from E. coli tRNA2(Gln)

Chemistry and Biology

Volumn 4, Issue 9, 1997, Pages 685-691

  Liu, David R ^a   Magliery, Thomas J ^a   Schultz, Peter G ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Aminoacyl tRNA synthetase; In vivo unnatural amino acid mutagenesis; Suppressor tRNA

Indexed keywords

ESCHERICHIA COLI;

EID: 0031239504     PISSN: 10745521     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1074-5521(97)90224-6     Document Type: Article

References (30)

1. Cornish, V.W., Mendel, D. & Schultz, P.G. (1995). Probing protein structure and function with an expanded genetic code. Angew. Chem. Int. Ed. Engl. 34, 621-633.
2. Mendel, D., Cornish, V.W. & Schultz, P.G. (1995). Site-directed mutagenesis with an expanded genetic code. Annu. Rev. Biophys. Biomol. Struct. 24, 435-462.
3. Thorson, J.S., Cornish, V.W., Barrett, J.E., Cload, ST., Yano, T. & Schultz, P.G. (1997). A biosynthetic approach for the incorporation of unnatural amino acids into proteins. Methods in Mol. Biol.: Protein Synthesis: Methods and Protocols, in press.
4. Nowak, N.W., et al., & Lester, H.A. (1995). Nicotinic receptor binding site probed with unnatural amino acid incorporation in intact cells. Science 268, 439-442.
5. Liu, D.R., Magliery, T.J., Pastrnak, M. & Schultz, P.G. (1997). Engineering a tRNA and aminoacyl-tRNA synthetase for the site-specific incorporation of unnatural amino acids into proteins in vivo. Proc. Natl Acad. Sci. USA, in press.
6. Rould, M.A., Perona, J.J. & Steitz, T.A. (1991). Structural basis of anticodon loop recognition by glutaminyl-tRNA synthetase. Nature 352, 213-218.
7. Sherman, JM, Thomann, H. & Söll, D. (1996). Functional connectivity between tRNA binding domains in glutaminyl-tRNA synthetase. J. Mol. Biol. 256, 818-828.
8. Gln by glutaminyl-tRNA synthetase: a complete identity set. EMBO J. 11, 4159-4165.
9. Gln are major recognition elements for E. coli glutaminyl-tRNA synthetase. Nature 352, 258-260.
10. Gln interaction. Biochimie 73, 1501-1508.
11. +2 mutants that increase amber suppression. J. Bacteriol. 145, 704-712.
12. Stewart, J, Wilson, D.B. & Ganem, B. (1990). A genetically engineered monofunctional chorismate mutase. J. Am. Chem. Soc. 112, 4582-4584.
13. Kleina, L.G., Masson, J.M., Normanly, J., Abelson, J. & Miller, J.H. (1990). Construction of Escherichia coli amber suppressor tRNA genes. II. Synthesis of additional tRNA genes and improvement of suppressor efficiency. J. Mol. Biol. 213, 705-717.
14. Neidhardt, F. & Umbarger, H.E. (1996). In Escherichia coli and Salmonella. (Neidherdt, F., ed.) pp. 13-16, ASM Press, Washington, D.C.
15. Ikemura, T. (1985). Codon usage and tRNA content in unicellular and multicellular organisms. Mol. Biol. Evol. 2, 13-34.
16. Raunio, R. & Rosenqvist, H. (1970). Amino acid pool of Escherichia coli during the different phases of growth. Acta. Chim. Scand. 24, 2737-2744.
17. McClain, W.H. (1993). Rules that govern tRNA identity in protein synthesis. J. Mol. Biol. 234, 257-280.
18. +2 glutamine tRNA in E. Coli. Jpn. J. Genet. 63, 237-249.
19. Gefter, M.L. & Russell, R.L. (1969). Role of modifications in tyrosine transfer RNA: a modified base affecting ribosome binding. J. Mol. Biol. 39, 145-147.
20. Ibba, M., Hong, K.-W., Sherman, J.M., Sever, S. & Soil, D. (1996). Interactions between tRNA identity nucleotides and their recognition sites in glutaminyl-tRNA synthetase determine the cognate amino acid affinity of the enzyme. Proc. Natl Acad. Sci. USA 93, 6953-6958.
21. RajBhandary, U.L. & Chow, C.M. (1995). In tRNA: Structure, Biosynthesis, and Function. (Söll, D. & RajBhandary, U.L., eds) p. 97, ASM Press, Washington.
22. RajBhandary, U.L. & Chow, C.M. (1995). In tRNA: Structure, Biosynthesis, and Function. (Söll, D. & RajBhandary, U.L., eds) p. 389, ASM Press, Washington.
23. Schulman, L.H. (1991). Recognition of tRNAs by aminoacyl-tRNA synthetases. Prog. Nucleic Acid Res. 41, 23-87.
24. Normanly, J. & Abelson, J. (1989). tRNA identity. Annu. Rev. Biochem. 58, 1029-1049.
25. Rogers, M.J., Adachi, T., Inokuchi, H. & Söll, D. (1994). Functional communication in the recognition of tRNA by Escherichia coli glutaminyl-tRNA synthetase. Proc. Natl Acad. Sci. USA 91, 291-295.
26. Weygand-Durasevic, I., Rogers, M.J. & Söll, D. (1994). Connecting anticodon recognition with the active site of Escherichia coli glutaminyl-tRNA synthetase. J. Mol. Biol. 240, 111-118.
27. Noren, C., Anthony-Cahill, S.J., Suich, D.J., Noren, K.A., Griffith, M.C. & Schultz, P.G. (1990). In vitro suppression of an amber mutation by a chemically aminoacylated transfer RNA prepared by runoff transcription. Nucleic Acids Res. 18, 83-88.
28. Cload, S.T., Liu, D.R., Froland, W.A. & Schultz, P.G. (1996). Development of improved tRNAs for in vitro biosynthesis of proteins containing unnatural amino acids. Chem. Biol. 3, 1033-1038.
29. Thomann, H.-U., Ibba, M., Hong, K.-W. & Söll, D. (1996). Homologous expression and purification of mutants of an essential protein by reverse epitope-tagging. Biotechnology 14, 50-55.
30. Lloyd, A.J., Thomann, H.-U., Ibba, M. & Söll, D. (1995). A broadly applicable continuous spectrophotometric assay for measuring aminoacyl-tRNA synthetase activity. Nucleic Acids Res. 23, 2886-2892.