Highly efficient production of enzymes of an extreme thermophile, Thermus thermophilus: A practical method to overexpress GC-rich genes in Escherichia coli

Extremophiles

Volumn 1, Issue 3, 1997, Pages 157-162

  Ishida, Masami ^a   Yoshida, Masasuke ^b   Oshima, Tairo ^c  

^a TOKYO UNIVERSITY OF MARINE SCIENCE AND TECHNOLOGY   (Japan)

^b TOKYO INSTITUTE OF TECHNOLOGY   (Japan)

^c TOKYO UNIVERSITY OF PHARMACY AND LIFE SCIENCES   (Japan)

Author keywords

3 Isopropylmalate dehydrogenase; GC rich gene; Gene overlapping; Leader open reading frame (ORF); Over production; Phosphofructokinase; Thermus thermophilus

Indexed keywords

3 ISOPROPYLMALATE DEHYDROGENASE; 6 PHOSPHOFRUCTOKINASE; ALCOHOL DEHYDROGENASE;

ARTICLE; BACTERIAL GENE; BIOSYNTHESIS; ENZYMOLOGY; ESCHERICHIA COLI; GENE; GENE EXPRESSION; GENETICS; MOLECULAR CLONING; OPEN READING FRAME; PLASMID; THERMUS THERMOPHILUS;

3-ISOPROPYLMALATE DEHYDROGENASE; ALCOHOL OXIDOREDUCTASES; CLONING, MOLECULAR; ESCHERICHIA COLI; GENE EXPRESSION; GENES, BACTERIAL; GENES, OVERLAPPING; OPEN READING FRAMES; PHOSPHOFRUCTOKINASE-1; PLASMIDS; THERMUS THERMOPHILUS;

EID: 0031196628     PISSN: 14310651     EISSN: None     Source Type: Journal    
DOI: 10.1007/s007920050029     Document Type: Article

References (22)

1. Almond J, Beecroft L, Blair E, Meacock P, Windass J (1985) Transformation of competent E. coli cells by plasmid DNA. In: Pritchard RH, Holland IB (eds) Basic cloning techniques: A manual of experimental procedures. Blackwell Scientific, London
2. Berkhout B, Schmidt BF, van Strien A, van Boom J, van Westrenen J, van Duin J (1987) Lysis gene of bacteriophage MS2 is activated by translation termination at the overlapping coat gene. J Mol Biol 195:517-524
3. Comeau DE, Ikenaka K, Tsung K, Inouye M (1985) Primary characterization of the protein products of the Escherichia coli ompB locus: Structure and regulation of synthesis of the OmpR and EnvZ proteins. J Bacteriol 164:578-584
4. Cox EC, Horner DL (1986) DNA sequence and coding properties of mutD (dnaQ) a dominant Escherichia coli mutator gene. J Mol Biol 190:113-117
5. Dean GE, Macnab RM, Stader J, Matsumura P, Burks C (1984) Gene sequence and predicted amino acid sequence of the motA protein, a membrane-associated protein required for flagellar rotation in Escherichia coli. J Bacteriol 159:991-999
6. Gold L, Stormo G (1987) Translational initiation. In: Neidhardt FC, Ingraham JL, Low KB, Magasanik B, Umbarger HE (eds) Escherichia coli and Salmonella typhimurium cellular and molecular biology. American Society for Microbiology, Washington, DC
7. Ishida M, Oshima T (1994) Overexpression of genes of an extreme thermophile, Thermus thermophilus, in Escherichia coli cells. J Bacteriol 176:2767-2770
8. Ishida M, Oshima T (1996) A leader open reading frame is essential for the expression in Escherichia coli of GC-rich leuB gene of an extreme thermophile, Thermus thermophilus. FEMS Microbiol Lett 135:137-142
9. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685
10. Lijestroem P, Laamanen I, Palva ET (1988) Structure and expression of the ompB operon, the regulatory locus for the outer membrane porin regulon in Salmonella typhimurium. J Mol Biol 201:663-673
11. Owen MJ (1984) DNA sequence determination using dideoxy analogs. In: Walker JM (ed) Methods in molecular biology. Humana, Clifton, NJ
12. Saiki RK, Gelfand DH, Stoffel S, Scharf SJ, Higuchi R, Horn GT, Mullis KB, Erlich HA (1988) Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239: 487-491
13. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: A laboratory manual, 2nd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
14. Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74:5463-5467
15. Schoner BE, Belagaje RM, Schoner RG (1986) Translation of a synthetic two-cistron mRNA in Escherichia coli. Proc Natl Acad Sci USA 83:8506-8510
16. Schoner BE, Belagaje RM, Schoner RG (1991) Enhanced translational efficiency with two-cistron expression system. Methods Enzymol 185:94-103
17. Stader J, Matsumura P, Vacante D, Dean GE, Macnab RM (1986) Nucleotide sequence of the Escherichia coli motB gene and site-limited incorporation of its product into the cytoplasmic membrane. J Bacteriol 166:244-252
18. Xu J, Oshima T, Yoshida M (1990) Tetramer-dimer conversion of phosphofructokinase from Thermus thermophilus induced by its allosteric effectors. J Mol Biol 215:597-606
19. Xu J, Seki M, Denda K, Yoshida M (1991) Molecular cloning of phosphofructokinase 1 gene from a thermophilic bacterium, Thermus thermophilus. Biochem Biophys Res Commun 176:1313-1318
20. Yamada T, Akutsu N, Miyazaki K, Kakinuma K, Yoshida M, Oshima T (1990) Purification, catalytic properties, and thermal stability of threo-Ds-3-isopropylmalate dehydrogenase coded by leuB gene from an extreme thermophile, Thermus thermophilus strain HB8. J Biochem 108:449-456
21. Yanisch-Perron C, Vieira J, Messing J (1985) Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33:103-119
22. Yanofsky C, Crawford IP (1987) The tryptophan operon. In: Neidhardt FC, Ingraham JL, Low KB, Magasanik B, Umbarger HE (eds) Escherichia coli and Salmonella typhimurium cellular and molecular biology. American Society for Microbiology, Washington, DC