A leader open reading frame is essential for the expression in Escherichia coli of GC-rich leuB gene of an extreme thermophile, Thermus thermophilus

FEMS Microbiology Letters

Volumn 135, Issue 1, 1996, Pages 137-142

  Ishida, Masami ^a   Oshima, Tairo ^b  

^a TOKYO UNIVERSITY OF MARINE SCIENCE AND TECHNOLOGY   (Japan)

^b TOKYO UNIVERSITY OF PHARMACY AND LIFE SCIENCES   (Japan)

Author keywords

Escherichia coli; Extreme thermophile; GC rich DNA; Gene expression; Leader open reading frame; Promoter tac; Thermus thermophilus

Indexed keywords

ARTICLE; CONTROLLED STUDY; ESCHERICHIA COLI; GENE EXPRESSION REGULATION; NONHUMAN; OPEN READING FRAME; PRIORITY JOURNAL; PROMOTER REGION; THERMUS THERMOPHILUS;

EID: 0030066911     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/0378-1097(95)00460-2     Document Type: Article

References (20)

1. Ishida, M. and Oshima, T. (1994) Overexpression of genes of an extreme thermophile, Thermus thermophilus, in Escherichia coli cells. J. Bacteriol. 176, 2767-2770.
2. Oshima, T. and Imahori, K. (1974) Description of Thermus thermophilus (Yoshida and Oshima) comb. nov., a nonsporulating thermophilic bacterium from a Japanese thermal spa. Int. J. Syst. Bacteriol. 24, 102-112.
3. Tanaka, T., Kawano, N. and Oshima, T. (1981) Cloning of 3-isopropylmalate dehydrogenase gene of an extreme thermophile and partial purification of the gene product. J. Biochem. 89, 677-682.
4. Close, T.J. and Rodriguez, R.L. (1982) Construction and characterization of the chloramphenicol-resistance gene cartridge: a new approach to the transcriptional mapping of extrachromosomal elements. Gene 20, 305-316.
5. Almond, J., Beecroft, L., Blair, E., Meacock, P. and Windass, J. (1985) Transformation of competent E. coli cells by plasmid DNA. In: Basic Cloning Techniques: A Manual of Experimental Procedures (Pritchard. R.H. and Holland, I.B., Eds.), pp. 35-38. Blackwell Scientific Publications, London.
6. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
7. Cobianchi, F. and Wilson, S.H. (1987) Enzymes for modifying and labeling DNA and RNA. Methods Enzymol. 152, 94-110.
8. Morrison, D.A. (1979) Transformation and preservation of competent bacterial cells by freezing. Methods Enzymol. 68, 326-331.
9. Rodriguez, R.L. and Tait, R.C. (1983) Recombinant DNA techniques: an introduction. Addison-Wesley Publishing Co., Massachusetts.
10. Yamada, T., Akutsu, N., Miyazaki K., Kakinuma, K., Yoshida, M. and Oshima, T. (1990) Purification, catalytic properties, and thermal stability of threo-Ds-3-isopropylmalate dehydrogenase coded by leuB gene from an extreme thermophile, Thermus thermophilus strain HB8. J. Biochem. 108, 449-456.
11. Kagawa, Y., Nojima, H., Nukima, N., Ishizuka, M., Nakajima, T., Yasuhara, T., Tanaka, T. and Oshima, T. (1984) High guanine plus cytosine content in the third letter of codons of an extreme thermophile. J. Biol. Chem. 259, 2956-2960.
12. Berkhout, B., Schmidt, B.F., van Strien, A., van Boom, J., van Westrenen, J. and van Duin, J. (1987) Lysis gene of bacteriophage M12 is activated by translation termination at the overlapping coat gene. J. Mol. Biol. 195, 517-524.
13. Comeau, D.E., Ikenaka, K., Tsung, K. and Inouye, M. (1985) Primary characterization of the protein products of the Escherichia coli ompB locus: structure and regulation of synthesis of the OmpR and EnvZ proteins. J. Bacteriol. 164, 578-584.
14. Gold, L. and Stormo, G. (1987) Translational initiation. In: Escherichia coli and Salmonella typhimurium Cellular and Molecular Biology (Neidhardt, P.C., Ingraham, J.L., Low, K.B., Magasanik, B. and Umbarger, H.E., Eds.), pp. 1302-1307. American Society for Microbiology, Washington, D.C.
15. Lijestroem, P., Laamanen, I. and Palva, E.T. (1988) Structure and expression of the ompB operon, the regulatory locus for the outer membrane porin regulon in Salmonella typhimurium. J. Mol. Biol. 201, 663-673.
16. Schoner, B.E., Belagaje, R.M. and Schoner, R.G. (1986) Translation of a synthetic two-cistron mRNA in Escherichia coli. Proc. Natl. Acad. Sci. USA 83, 8506-8510.
17. Spanjaard, R.A. and van Duin, J. (1989) Translational reinitiation in the presence and absence of a Shine and Dalgarno sequence. Nucleic Acids Res. 17, 5501-5507.
18. Yanofsky, C. and Crawford, I.P. (1987) The tryptophan operon. In: Escherichia coli and Salmonella typhimurium Cellular and Molecular Biology (Neidhardt. F.C., Ingraham, J.L., Low, K.B., Magasanik, B. and Umbarger, H.E., Eds.), pp. 1453-1472. American Society for Microbiology, Washington, D.C.
19. Schmidt, B.F., Berkhout, B., Overbeek, G.P., van Strien, A. and van Duin, J. (1987) Determination of the RNA secondary structure that regulates lysis gene expression in bacteriophage MS2. J. Mol. Biol. 195, 505-516.
20. Kozak, M. (1983) Compilation and analysis of sequences upstream from the translational start site in eukaryotic mRNAs. Microbiol. Rev. 47, 1-45.