Analysis of transgene integration sites in transgenic pigs by fluorescence in situ hybridization

Transgenic Research

Volumn 6, Issue 4, 1997, Pages 253-259

  Kuipers, Henriëtte W ^a   Langford, Gillian A ^a   White, David J G ^a  

^a UNIVERSITY OF CAMBRIDGE   (United Kingdom)

Author keywords

Fluorescence in situ hybridization; Human decay accelerating lacier; Transgenic pigs

Indexed keywords

ANIMALIA; SUS SCROFA;

EID: 0031194554     PISSN: 09628819     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1018402510471     Document Type: Article

References (20)

1. Cooper, D.K.C., Ye, Y., Rolf, L.L. Jr. and Zuhdi, N. (1991) The pig as potential organ donor for man. In Cooper, D.K.C., Kemp, E., Reemtsma, K. and White, D. J. eds The Transplantation of Organs and Tissues Between Species Springer-Verlag p. 481.
2. Cozzi, E., Langford, G.A., Wright, L., Tucker, A., Yannoutsos, N., Richards, A.C., Rosengard, A., Elsome, K., Lancaster, R. and White, D.J.G. (1995) Comparative analysis of human DAF expression in the tissues of transgenic pigs and man. Transpl. Proc. 27, 319-20.
3. Dinchuk, J.E., Kelley, K. and Boyle, A.L. (1994) Fluorescence in situ hybridisation of interphase nuclei isolated from whole blood of transgenic mice. BioTechniques 17, 954-9.
4. Dycaico, M.J., Provost, G.S., Kretz, P.L., Ransom, S.L., Moores, J.C. and Short, J.M. (1994) The use of shuttle vectors for mutation analysis in transgenic mice and rats. Mutation Res. 307, 461-78.
5. Fodor, W.L., Williams, B.L., Matis, L.A., Madri, J.A., Rollins, S.A., Knight, J.W., Velander, W. and Squinto, S.P. (1994) Expression of a functional human complement inhibitor in a transgenic pig as a model for the prevention of xenogeneic hyperacute organ rejection. Proc. Natl Acad. Sci. USA 91, 11153-7.
6. Langford, C.F., Telenius, H., Miller N.G.A., Thomsen, P.D. and Tucker, E.M. (1993) Preparation of chromosome-specific paints and complete assignment of chromosomes in the pig flow karyotype. Animal Genet. 24, 261-7.
7. Langford, G.A., Yannoutsos, N., Cozzi, E., Lancaster, R., Elsome, K., Chen, P., Richards, A. and White, D.J.G. (1994) Production of pigs transgenic for human decay accelerating factor. Transpl. Proc. 26, 1400-1.
8. Lee, J.E., Tantravahi, U., Boyle, A.L. and Efstratiadis, A. (1993) Parental imprinting of an IGF-2 transgene. Mol. Reprod. Develop. 35, 382-90.
9. McCurry, K.R., Kooyman, D.L., Alvarado, C.G., Cotterell, A.H., Martin, M.J., Logan, J.S. and Platt, J.L. (1995) Human complement regulatory proteins protect swine-to-primate cardiac xenografts from humoral injury. Nature Med. 1, 423-7.
10. Nishino, H., Herath, J.F., Jenkins, R.B. and Sommer, S.S. (1995) Fluorescence in situ hybridisation for rapid differentiation of zygosity in transgenic mice. BioTechniques 19, 587-92.
11. Pardue, M.L. and Gall, J.G. (1975) Nucleic acid hybridisation to the DNA of cytological preparations. Methods Cell Biol. 10, 1-16.
12. Paris, D., Toyama, K., Megarbané, A., Casanova, M., Sinet, P.M. and London, J. (1996) Rapid fluorescence in situ hybridisation on interphase nuclei to discriminate between homozygous and heterozygous transgenic mice. Transgenic Res. 5, 1-7.
13. Pinkel, D., Landegent, J., Collins, C., Fuscoe, J., Segraves, R., Lucas, J. and Gray, J. (1988) Fluorescence in situ hybridisation with human chromosome specific libraries: detection of trisomy 21 and translocations of chromosome 4. Proc. Natl. Acad. Sci. USA 85, 9138-42.
14. Pierson, R.N., Pino-Chavez, G., Young, V.K., Kasper-Konig, W., White, D.J.G. and Wallwork, J. (1996) Expression of human decay accelerating factor may protect pig lung from hyperacute rejection by human blood. J. Heart Lung Transplant. In press.
15. Platt, J.L., Vercellotti, G.M., Dalmasso, A.P., Matas, A.J., Bolman, R.M., Najarian, J.S. and Bach, F.H. (1990) Transplantation of discordant xenografts: a review in progress. Immunol. Today 11, 450-7.
16. Rosengard, A.M., Cary, N.R.B., Langford, G.A., Tucker, A.W., Wallwork, J. and White, D.J.G. (1995) Tissue expression of human complement inhibitor, decay accelerating factor, in transgenic pigs - a potential approach for preventing xenograft rejection. Transplantation 59, 1325-33.
17. Shi, Y.P., Huang, T.T., Carlson, E.J. and Epstein, C.J. (1994) The mapping of transgenes by fluorescence in situ hybridisation on G-banded mouse chromosomes. Mamm. Genome 5, 337-41.
18. Steel, C.M. (1994) Identification and characterisation of cancer genes. Br. Med. Bulletin 50, 536-59.
19. Swiger, R.R., Tucker, J.D. and Heddle, J.A. (1995) Detection of transgenic animals without cell culture using fluorescence in situ hybridization. BioTechniques 18, 952-8.
20. White, D.J.G., Langford, G.A., Cozzi, E. and Young, V.J. (1995) Production of pigs transgenic for human DAF. A strategy for xenotransplantation. Xenotransplantation 2, 213-17.