Rapid fluorescence in situ hybridization on interphasic nuclei to discriminate between homozygous and heterozygous transgenic mice

Transgenic Research

Volumn 5, Issue 6, 1996, Pages 397-403

  Paris, Daniel ^a   Toyama, Kiyoko ^a   Mégarbané, André ^b   Casanova, Myriam ^a   Sinet, Pierre Marie ^a   London, Jacqueline ^a  

^a CNRS   (France)

^b HÔPITAL NECKER ENFANTS MALADES   (France)

Author keywords

FISH on mouse interphasic nuclei; rapid hSOD 1 assay; transgenic inbred FVB N hSOD 1 mice

Indexed keywords

ANIMALIA; MUS MUSCULUS;

EID: 0030297843     PISSN: 09628819     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF01980204     Document Type: Article

References (24)

1. Barkats, M., Bertholet, J.Y., Venault, P., Ceballos-Picot, I., Nicole, A., Phillips, J., Moutier, R., Roubertoux, P., Sinet, P.M. and Cohen-Salmon, C. (1993) Hippocampal mossy fiber changes in mice transgenic for the human copper-zinc superoxide dismutase gene. Neurosci. Lett. 160, 24-8.
2. Beauchamp, C. and Fridovich, I. (1987) Superoxide dismutase: improved assays applicable to acrylamide gels. Anal. Biochem. 44, 276-87.
3. Boyle, A.L., Feltquite, D.M., Dracopoli, N.C., Housman, D.E. and Ward, D.C. (1992) Rapid physical mapping of cloned DNA on banded mouse chromosomes by fluorescence in situ hybridization. Genomics 12, 106-15.
4. Blouin, J.L., Rahmani, Z., Chettounh, Z., Prieur, M., Fermanian, J., Poissonier, M., Leonard, C., Nicole, A., Mattei, J.F., Sinet, P.M. and Delabar, J.M. (1990) Slot blot method for the quantification of DNA sequences and mapping of chromosome rearrangements: application to chromosome 21. Am. J. Hum. Genet. 46, 518-526.
5. Ceballos, I., Delabar, J.M., Nicole, A., Kamoun, P. and Sinet, P.M. (1988) Expression of transfected human CuZn superoxyde dismutase in mouse L cells and NS20Y neuroblastoma cells induces enhancements of glutathione peroxydase activity. Biochim. Biophys. Acta. 949, 58-64.
6. Ceballos-Picot, I., Nicole, A., Briand, P., Grimber, G., Delacourte, A., Defossez, A., Javoy-Agid, F., Lafon, M., Blouin, J.L. and Sinet, P.M. (1991) Neuronal specific expression of human copper-zinc superoxide dismutase gene in transgenic mice: animal model of gene dosage effects of Down's syndrome. Brain Res. 552, 198-214.
7. Davisson, M.T. and Akeson, E.C. (1987) An improved method for preparing G-banded chromosomes from mouse peripheral blood. Cytogenet. Cell Genet. 45, 70-4.
8. Elroy-Stein, O., Bernstein, Y. and Groner, Y. (1986) Overproduction of human Cu/Zn-superoxide dismutase in transfected cells: extenuation of paraquat-mediated cytotoxicity and enhancement of lipid peroxidation. EMBO J. 5, 615-22.
9. Epstein, C.J., Avraham, K.B., Lovett, M., Smith, S., Elroy-Stein, O., Rotman, G., Bry, C. and Groner, Y. (1987) Transgenic mice with increased CuZn-superoxide dismutase activity: animal model of dosage effects in Down syndrome. Proc. Natl Acad. Sci. USA 84, 8044-8.
10. Feinberg, A.P. and Vogelstein, B. (1986) A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal. Biochem. 132, 6-13.
11. Grimber, G., Galand, C., Garbarz, M., Mattei, M.G., Cavard, C., Zider, A., Blanchet, P., Boivin, P., Briand, P. and Dhermy, D. (1992) Inherited haemolytic anaemia created by insertional inactivation of the a-spectrin gene. Transgenic Res. 1, 268-74.
12. Hallewell, R., Puma, J.P., Mullenback, G.T. and Najarian, C. (1986) Structure of the human CuZn SOD gene. In Rotilio G. ed., Superoxide Dismutase in Chemistry, Biology and Medecine, pp. 249-256. Amsterdam, Netherlands: Elsevier Science Publishers.
13. Hogan, B., Costantini, F. and Lacy, E. (1986) Manipulating the Mouse Embryo. A Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.
14. Kubisch, H., Wang, J., Luche, R., Carlson, E., Bray, T.M., Epstein, C.J. and Philipps, J.P. (1994) Transgenic copper/zinc superoxide dismutase modulates susceptibility to type I diabetes. Proc. Natl Acad. Sci. USA 91, 9956-9.
15. Levanon, D., Lieman-Hurwitz, J., Dafni, N., Wigderson, M., Sherman, L., Bernstein, Y., Laver-Rudich, Z., Danciger, E., Stein, O. and Groner, Y. (1985) Architecture and anatomy of the chromosomal locus in human chromosome 21 encoding the Cu/Zn superoxide dismutase. EMBO J. 4, 77-84.
16. London, J., Toyama, K., Paris, D., Paly, E., Casanova, M. and Sinet, P.M. (1993) The use of FVB/N inbred strain to generate transgenic progeny with the same genetic background. Biol. Cell. 77, 335.
17. Meisler, M.H. (1992) Insertional mutation of classical and novel genes in transgenic mice. Trends Genet. 8, 341-4.
18. Péquignot, E.V (1992) In situ hybridization to chromosomes with biotinylated probes. In Wilkinson, D.G. ed. In situ Hybridization: a Practical Approach, pp 137-58, Oxford, UK: IRL Press Ltd.
19. Ratty, A.K., Fitzgerald, L.W., Titeler, M., Glick, S.D., Mullins, J.J. and Gross, K.W. (1990) Circling behavior exhibited by a transgenic insertional mutant. Mol. Brain Res. 8, 355-8.
20. Shi, Y.P., Huang, T.T., Carlson, E.J. and Epstein, C.J. (1994) The mapping of transgenes by fluorescence in situ hybridization on G-banded mouse chromosomes. Mamm. Genome 5, 337-41.
21. Taketo, M., Schroeder, A.C., Mobraaten, L.E., Gunning, K.B., Hanten, G., Fox, R.R., Roderick, T.H., Stewart, C.L., Lilly, F. Hansen, C.T. and Overbeek, P.A. (1991) FVB/N: an inbred mouse strain preferable for transgenic analyses. Proc. Natl Acad. Sci. USA 88, 2065-9.
22. Tsuchihashi, M. (1923) Zur Kenntnis des Blutkatalase. Biochem. Z. 140, 63-112.
23. Vos, S., Epstein, C.J., Carlson, E., Cho, S.K. and Koeffler, H.P. (1995) Transgenic mice overexpressing human copper/zinc-superoxide dismutase (Cu/Zn SOD) are not resistant to endotoxic shock. Biochem. Biophys. Res. Commun. 208, 523-31.
24. Wiegant, J., Kalle, W., Mullenders, L., Brookes, S., Hoovers, J.M.N., Dauwerse, J.G., Van Ommen, G.J.B. and Raap, A.K. (1992) High-resolution in situ hybridization using DNA halo preparations. Hum. Mol. Genet. 1, 587-91.