Transmembrane phospholipid distribution revealed by freeze-fracture replica labeling

Journal of Cell Science

Volumn 109, Issue 10, 1996, Pages 2453-2460

  Fujimoto, Kazushi ^a   Umeda, Masato ^b   Fujimoto, Toyoshi ^a,c  

^a KYOTO UNIVERSITY   (Japan)

^b TOKYO METROPOLITAN INSTITUTE OF MEDICAL SCIENCE   (Japan)

^c GUNMA UNIVERSITY   (Japan)

Author keywords

Freeze fracture replica labeling; Immunogold electron microscopy; Membrane; Phospholipid

Indexed keywords

MEMBRANE PHOSPHOLIPID;

ARTICLE; CELL NUCLEUS; ELECTRON MICROSCOPY; ENDOPLASMIC RETICULUM; ERYTHROCYTE MEMBRANE; FREEZE FRACTURE; HUMAN; HUMAN CELL; IMMUNOCYTOCHEMISTRY; IMMUNOGOLD STAINING; INTRACELLULAR MEMBRANE; MITOCHONDRION; MYELIN SHEATH; NERVE CELL; NORMAL HUMAN; PHOTORECEPTOR CELL; PRIORITY JOURNAL; SECRETORY GRANULE;

ANIMALS; CALCIMYCIN; ERYTHROCYTE MEMBRANE; FREEZE FRACTURING; HUMANS; IMMUNOHISTOCHEMISTRY; LIVER; MEMBRANES, ARTIFICIAL; MICROSCOPY, ELECTRON; MYELIN SHEATH; OPTIC NERVE; PANCREAS; PHOSPHATIDYLCHOLINES; PHOSPHOLIPIDS; RATS;

EID: 0029969182     PISSN: 00219533     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (19)

1. Andersson-Forsman, C. and Pinto da Silva, P. (1988). Fracture-flip: new high-resolution images of cell surfaces after carbon stabilization of freeze-fractured membranes. J. Cell Sci. 90, 531-541.
2. Branton, D., Bullivant, S., Gilula, N. B., Karnovsky, M. J., Moor, H., Muhlethaler, K., Northcote, D. H., Packer, L., Satir, P., Speth, V., Staehelin, L. A., Steere, R. L. and Weinstein, R. S. (1975). Freeze-etching nomenclature. Science 190, 54-56.
3. Fujimoto, K. and Pinto da Silva, P. (1988). Macromolecular dynamics of the cell surface during the formation of coated pits is revealed by fracture-flip. J. Cell Sci. 91, 161-173.
4. Fujimoto, K. and Pinto da Silva, P. (1989). Surface views of nuclear pores in isolated rat liver nuclei as revealed by fracture-flip/Triton-X. Eur. J. Cell Biol. 50, 390-397.
5. Fujimoto, K. and Ogawa, K. (1991). Fracture-flip and fracture-flip cytochemistry to study macromolecular architecture of membrane surfaces: practical procedures, interpretation and application. Acta Histochem. Cytochem. 24, 111-117.
6. Fujimoto, K. and Pinto da Silva, P. (1992). Fracture-flip/Triton X-100 reveals the cytoplasmic surface of human erythrocyte membranes. Acta Histochem. Cytochem. 25, 255-263.
7. Fujimoto, K. (1995). Freeze-fracture replica electron microscopy combined with SDS digestion for cytochemical labeling of integral membrane proteins. Application to the immunogold labeling of intercellular junctional complexes. J. Cell Sci. 108, 3443-3449.
8. Guidotti, G. (1972). Membrane proteins. Annu. Rev. Biochem. 41, 731-752.
9. Heuser, J. E., Reese, T. S., Jan, L. Y., Dennis, M. J. and Evans, L. (1979). Synaptic vesicle exocytosis captured by quick-freezing and correlated with quantal transmitter release. J. Cell Biol. 81, 275-300.
10. 2+, phosphate and calcium-phosphate complexes. Eur. J. Biochem. 146, 131-140.
11. Kinsky, S. C., Haxby, J. A., Zopf, D. A., Alving, C. R. and Kinsky, C. B. (1969). Complement-dependent damage to liposomes prepared from pure lipids and Forssman hapten. Biochemistry 8, 4149-4158.
12. Nam, K. S., Igarashi, K., Umeda, M. and Inoue, K. (1990). Production and characterization of monoclonal antibodies that specifically bind to phosphatidylcholine. Biochim. Biophys. Acta 1046, 89-96.
13. Pinto da Silva, P. and Kachar, B. (1982). On tight-junction structure. Cell 28, 441-450.
14. Schrier, S. L., Zachowski, A., Herve, P., Kader, J.-P. and Devaux, P. F. (1992). Transmembrane redistribution of phospholipids of the human red cell membrane during hypotonic hemolysis. Biochim. Biophys. Acta 1105, 170-176.
15. Simons, K. and van Meer, G. (1988). Lipid sorting in epithelial cells. Biochemistry 27, 6197-6202.
16. Sjöstrand, F. S. and Kreman, M. (1979). Freeze-fracture analysis of structure of plasma membrane of photoreceptor cell outer segments. J. Ultrastruct. Res. 66, 254-275.
17. 2+ induces transbilayer redistribution of all major phospholipids in human erythrocytes. Biochemistry 31, 6355-6360.
18. Zachowski, A. (1993). Phospholipids in animal eukaryotic membranes: transverse asymmetry and movement. Biochem. J. 294, 1-14.
19. Zhou, X. and Arthur, G. (1992). Improved procedures for the determination of lipid phosphorus by malachite green. J. Lipid Res. 33, 1233-1236.