γ-tubulin in trypanosomes: Molecular characterisation and localisation to multiple and diverse microtubule organising centres

Journal of Cell Science

Volumn 110, Issue 2, 1997, Pages 157-168

  Scott, V ^a   Sherwin, T ^a   Gull, K ^a  

^a UNIVERSITY OF MANCHESTER   (United Kingdom)

Author keywords

tubulin; Cytoskeleton; MTOC; Trypanosome

Indexed keywords

HYBRID PROTEIN; POLYADENYLATED RNA; POLYCLONAL ANTIBODY; TUBULIN;

ARTICLE; CENTRIOLE; CENTROSOME; IMMUNOFLUORESCENCE; MICROTUBULE; MOLECULAR CLONING; NONHUMAN; NORTHERN BLOTTING; PRIORITY JOURNAL; SEQUENCE HOMOLOGY; TRYPANOSOMA BRUCEI;

AMINO ACID SEQUENCE; ANIMALS; ANTIBODIES, PROTOZOAN; BASE SEQUENCE; CERCOPITHECUS AETHIOPS; GENE EXPRESSION; HUMANS; MICROTUBULE-ASSOCIATED PROTEINS; MICROTUBULES; MOLECULAR SEQUENCE DATA; PROTOZOAN PROTEINS; RATS; RNA, MESSENGER; SEQUENCE ANALYSIS, DNA; SEQUENCE HOMOLOGY, AMINO ACID; TRYPANOSOMA BRUCEI BRUCEI; TUBULIN; VERO CELLS;

ESCHERICHIA COLI; MAMMALIA; PLASMODIUM FALCIPARUM; TRYPANOSOMA; TRYPANOSOMA BRUCEI; XENOPUS LAEVIS;

EID: 0031049074     PISSN: 00219533     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (57)

1. Bastin, P., Matthews, K. R. and Gull, K. (1996). The paraflagellar rod of kinetoplastida: Solved and unsolved questions. Parasitol. Today 12, 302-307.
2. Brun, R. and Schönenburger, M. (1979). Cultivation and in vitro cloning of procyclic culture forms of Trypanosoma brucei in a semi-defined medium. Acta Tropica 36, 289-292.
3. Burns, R. G. and Surridge, C. D. (1994). Functional role of a consensus peptide which is common to α-, β- and γ-tubulin, to actin and centractin, to phytochrome A, and to the TCP1α chaperonin protein. FEBS Lett. 347, 105-111.
4. Burns, R. G. (1995a). Analysis of the γ-tubulin sequences: implications for the functional properties of γ-tubulin. J. Cell Sci. 108, 2123-2130.
5. Burns, R. G. (1995b). Identification of two new members of the tubulin family. Cell Motil. Cytoskel. 31, 255-258.
6. Chomczynski, P. and Sacchi, N. (1987). Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162, 156-159.
7. Dibbayawan, T. P., Harper, J. D. I., Elliot, J. E., Gunning, B. E. S. and Marc, J. (1995). A γ-tubulin that associates specifically with centrioles in HeLa cells and the basal body complex in Chlamydomonas. Cell Biol. Int. 19, 559-567.
8. Ersfeld, K., Wehland, J., Plessmann, U., Dodemont, H., Gerke, V. and Weber, K. (1993). Characterisation of the tubulin tyrosine ligase. J. Cell Biol. 120, 725-732.
9. Feinberg, A. P. and Vogelstein, B. (1983). A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal. Biochem. 132, 6-13.
10. Felix, M.-A., Antony, C., Wright, M. and Maro, B. (1994). Centrosome assembly in vitro: role of γ-tubulin recruitment in Xenopus sperm aster formation. J. Cell Biol. 124, 19-31.
11. Fuchs, U., Moepps, B., Maucher, H. P. and Schraudolf, H. (1993). Isolation, characterisation and sequence of a cDNA encoding γ-tubulin protein from the fern Anemia phyllitidis L. Sw. Plant Mol. Biol. 23, 595-603.
12. Fuller, S. D., Gowen, B. E., Reinsch, S., Sawyer, A., Buendia, B., Wepf, R. and Karsenti, E. (1995). The core of the mammalian centriole contains gamma-tubulin. Curr. Biol. 5, 1384-1393.
13. Gueth-Hallonet, C., Antony, C., Aghion, J., Santa-Maria, A., Lajoie-Mazenc, I., Wright, M. and Maro, B. (1993). γ-Tubulin is present in acentriolar MTOCs during early mouse development. J. Cell Sci. 105, 157-166.
14. Havercroft, J. C. and Gull, K. (1983). Demonstration of different patterns of microtubule organization in Physarum polycephalum myxamoebae and plasmodia using immunofluorescence microscopy. Eur. J. Cell Biol. 32, 67-74.
15. Hoffman, J. C., Vaughn, K. C. and Joshi, H. C. (1994). Structural and immunocytochemical characterization of microtubule-organizing centers in Pteridophyte spermatogenous cells. Protoplasma 179, 46-60.
16. Horio, T., Uzawa, S., Jung, M. K., Oakley, B. R., Tanaka, K. and Yanagida, M. (1991). The fission yeast γ-tubulin is essential for mitosis and is localised at microtubule organising centres. J. Cell Sci. 99, 693-700.
17. Horio, T. and Oakley, B. R. (1994). Human γ-tubulin functions in fission yeast. J. Cell Biol. 126, 1465-1473.
18. Imboden, M. A., Laird, P. W., Affolter, M. and Seebeck, T. (1987). Transcription of the intergenic regions of the tubulin cluster of Trypanosoma brucei: evidence for a polycistronic transcription unit in a eukaryote. Nucl. Acids Res. 15, 7357-7368.
19. Joshi, H. C., Palacios, M. J., McNamara, L. and Cleveland, D. W. (1992). γ-Tubulin is a centrosomal protein required for cell cycle-dependent microtubule nucleation. Nature 356, 80-83.
20. Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685.
21. Lajoie-Mazenc, I., Tollon, Y., Detraves, C., Julian, M., Moisand, A., Gueth-Hallonet, C., Debec, A., Salles-Passador, I., Puget, A., Mazarguil, H., Raynaud-Messina, B. and Wright, M. (1994). Recruitment of antigenic gamma-tubulin during mitosis in animal cells: presence of gamma-tubulin in the mitotic spindle. J. Cell Sci. 107, 2825-2837.
22. Lange, B. M. H., Sherwin, T., Hagan, I. M. and Gull, K. (1995). The basics of immunofluorescence video microscopy for mammalian and microbial systems. Trends Cell Biol. 5, 328-332.
23. Lange, B. M. H. and Gull, K. (1996). Structure and function of the centriole in animal cells: progress and questions. Trends Cell Biol. 6, 348-352.
24. Liang, A. and Heckmann, K. (1993). The macronuclear γ-tubulin-encoding gene of Euplotes octocarinatus contains two introns and an in-frame TGA. Gene 136, 319-322.
25. Liang, A., Ruiz, F., Heckmann, K., Klotz, C., Tollon, Y., Beisson, J. and Wright, M. (1996). Gamma-tubulin is permanently associated with basal bodies in ciliates. Eur. J. Cell Biol. 70, 331-338.
26. Liu, B., Marc, J., Joshi, H. C. and Palevitz, B. A. (1993). A γ-tubulin-related protein associated with the microtubule arrays of higher plants in a cell cycle-dependent manner. J. Cell Sci. 104, 1217-1228.
27. Maessen, S., Wesseling, J. G., Smits, M. A., Konings, R. N. H. and Schoenmakers J. G. G. (1993). The γ-tubulin gene of the malaria parasite Plasmodium falciparum. Mol. Biochem. Parasitol. 60, 27-36.
28. Marschall, L. G., Jeng, R. L., Mulholland, J. and Stearns, T. (1996). Analysis of Tub4p, a yeast γ-tubulin-like protein: Implications for microtubule-organizing center function. J. Cell Biol. 134, 443-454.
29. Masuda, H., Sevik, M. and Cande, W. Z. (1992). In vitro microtubule-nucleating activity of spindle pole bodies in fission yeast Schizosaccharomyces pombe: cell cycle-dependent activation in Xenopus cell-free extracts. J. Cell Biol. 117, 1055-1066.
30. Moritz, M., Braunfeld, M. B., Sedat, J. W., Alberts, B. and Agard, D. A. (1995). Microtubule nucleation by γ-tubulin-containing rings in the centrosome. Nature 378, 638-640.
31. Moudjou, M., Bordes, N., Paintrand, M. and Bornens, M. (1996). γ-Tubulin in mammalian cells: the centrosomal and the cytosolic forms. J. Cell Sci. 109, 875-887.
32. Muresan, V., Joshi, H. C. and Besharse, J. C. (1993). γ-Tubulin in differentiated cell types: localisation in the vicinity of basal bodies in retinal photoreceptors and ciliated epithelia. J. Cell Sci. 104, 1229-1237.
33. Nováková, M., Dráberová, E., Schürmann, W., Czihak, G., Viklicky, V. and Dráber, P. (1996). γ-Tubulin redistribution in taxol-treated mitotic cells probed by monoclonal antibodies. Cell Motil. Cytoskel. 33, 38-51.
34. Oakley, C. E. and Oakley, B. R. (1989). Identification of γ-tubulin, a new member of the tubulin superfamily encoded by mipA gene of Aspergillus nidulans. Nature 338, 662-664.
35. Oakley, B. R., Oakley, C. E., Yoon, Y. and Jung, M. K. (1990). γ-Tubulin is a component of the spindle pole body that is essential for microtubule function in Aspergillus nidulans. Cell 61, 1289-1301.
36. Palacios, M. J., Joshi, H. C., Simerly, C. and Schatten, G. (1993). γ-Tubulin reorganisation during mouse fertilisation and early development. J. Cell Sci. 104, 383-389.
37. Pickett-Heaps, J. D. (1969). The evolution of the mitotic apparatus: An attempt at comparative ultrastructural cytology in dividing plant cells. Cytobios 3, 257-280.
38. Robinson, D. R. and Gull, K. (1991). Basal body movement as a mechanism for mitochondrial genome segregation in the trypanosome cell cycle. Nature 352, 731-734.
39. Robinson, D. R., Sherwin, T., Ploubidou, A., Byard, E. H. and Gull, K. (1995). Microtubule polarity and dynamics in the control of organelle positioning, segregation and cytokinesis in the trypanosome cell cycle. J. Cell Biol. 128, 1163-1172.
40. Sambrook, J., Fritsch, E. F. and Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual. 2nd edn. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press.
41. Sanger, F., Nicklen, S. and Coulson, A. R. (1977). DNA sequencing with chain terminating inhibitors. Proc. Nat. Acad. Sci. USA 74, 5463-5467.
42. Sehedl, T., Burland, T. G., Gull, K. and Dove, W. F. (1984). Cell cycle regulation of tubulin RNA level, tubulin protein synthesis, and assembly of microtubules in Physarum. J. Cell Biol. 99, 155-165.
43. Schlaeppi, K., Deflorin, J. and Seebeck, T. (1989). The major component of the paraflagellar rod of Trypanosoma brucei is a helical protein that is encoded by two identical, tandemly linked genes. J. Cell Biol. 109, 1695-1709.
44. Schoeneberg, U., Vahrson, W., Priedemuth, U. and Wittig, B. (1994). Analysis and interpretation of DNA and protein sequences using MacMolly Tetra. 3rd. Edition, KAROI-Verl. Bornemann.
45. Seebeck, T., Whittaker, P. A., Imboden, M. A., Hardman, N. and Braun, R. (1983). Tubulin genes of Trypanosoma brucei: a tightly clustered family of alternating genes. Proc. Nat. Acad. Sci. USA 80, 4634-4638.
46. Sherwin, T., Schneider, A., Sasse, R., Seebeck, T. and Gull, K. (1987). Distinct localization and cell cycle dependence of COOH terminally tyrosinolated α-tubulin in the microtubules of Trypanosoma brucei brucei. J. Cell Biol. 104, 439-446.
47. Sherwin, T. and Gull, K. (1989a). The cell division cycle of Trypanosoma brucei brucei: timing of event markers and cytoskeletal modulations. Phil. Trans. Roy. Soc. Lond. B. 323, 573-588.
48. Sherwin, T. and Gull, K. (1989b). Visualization of detyrosination along single microtubules reveals novel methods of assembly during cytoskeletal duplication in trypanosomes. Cell 57, 211-221.
49. Shu, H.-B. and Joshi, H. C. (1995). γ-Tubulin can both nucleate microtubule assembly and self-assemble into novel tubular structures in mammalian cells. J. Cell Biol. 130, 1137-1147.
50. Sobel, S. G. and Snyder, M. (1995). A highly divergent γ-tubulin gene is essential for cell growth and proper microtubule organization in Saccharomyces cerevisiae. J. Cell Biol. 131, 1775-1788.
51. Spang, A., Geissler, S., Grein, K. and Schiebel, E. (1996). γ-Tubulin-like Tub4p of Saccharomyces cerevisiae is associated with the spindle pole body substructures that organise microtubules and is required for mitotic spindle function. J. Cell Biol. 134, 429-441.
52. Stearns, T., Evans, L. and Kirschner, M. (1991). γ-Tubulin is a highly conserved component of the centrosome. Cell 65, 825-836.
53. Stearns, T. and Kirschner, M. (1994). In vitro reconstruction of centrosome assembly and function: the central role of γ-tubulin. Cell 76, 623-637.
54. Sunkel, C. E., Gomes, R., Sampaio, P., Perdigao, J. and Gonzalez, C. (1995). γ-Tubulin is required for the structure and function of the microtubule organizing center in Drosophila neuroblasts. EMBO J. 14, 28-36.
55. Thomashow, L. S., Milhausen, M., Rutter, W. J. and Agabian, N. (1983). Tubulin genes are tandemly linked and clustered in the genome of Trypanosoma brucei. Cell 32, 35-43.
56. Woodward, R. and Gull, K. (1990). Timing of nuclear and kinetoplast DNA replication and early morphological events in the cell cycle of Trypanosoma brucei. J. Cell Sci. 95, 49-57.
57. Zheng, Y., Jung, M. K. and Oakley, B. R. (1991). γ-Tubulin is present in Drosophila melanogaster and Homo sapiens and is associated with the centrosome. Cell 65, 817-823.