The Chlamydia trachomatis parasitophorous vacuolar membrane is not passively permeable to low-molecular-weight compounds

Infection and Immunity

Volumn 65, Issue 3, 1997, Pages 1088-1094

  Heinzen, Robert A ^a,b   Hackstadt, Ted ^a  

^a NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES   (United States)

^b UNIVERSITY OF WYOMING   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

FLUORESCENT DYE;

ANIMAL CELL; ARTICLE; CELL INCLUSION; CHLAMYDIA TRACHOMATIS; FLUORESCENCE MICROSCOPY; INTRACELLULAR MEMBRANE; LASER MICROSCOPY; MEMBRANE PERMEABILITY; MOLECULAR SIZE; NONHUMAN; PRIORITY JOURNAL;

EID: 0031035718     PISSN: 00199567     EISSN: None     Source Type: Journal    
DOI: 10.1128/iai.65.3.1088-1094.1997     Document Type: Article

References (38)

1. Baca, O. G., and D. Paretsky. 1983. Q fever and Coxiella burnetii: a model for host-parasite interactions. Mierobiol. Rev. 47:127-149.
2. Beckers, C. J. M., J. F. Dubremetz, O. Mercereau-Puijalon, and K. A. Joiner. 1994. The Toxoplasnta gondii rhoptry protein ROP 2 is inserted into the parasitophorous vacuole membrane, surrounding the intracellular parasite, and is exposed to the host cell cytoplasm. J. Cell Biol. 127:947-961.
3. Bielecki, J., P. Youngman, P. Connelly, and D. A. Portnoy. 1990. Bacillus subtilis expressing a haemolysin gene from Listeria monocytogenes can grow in mammalian cells. Nature 345:175-176.
4. Caldwell, H. D., J. Kromhout, and J. Schachter. 1981. Purification and partial characterization of the major outer membrane protein of Chlamydia trachomatis. Infect. Immun. 31:1161-1176.
5. Clemens, D. L., and M. A. Horwitz. 1996. The Mycobacterium tuberculosis phagosome interacts with early endosomes and is accessible to exogenously administered transferrin. J. Exp. Med. 184:1349-1355.
6. Cormack, B. P., R. H. Valdivia, and S. Falkow. 1996. FACS-optimized mutants of the green fluorescent protein (GFP). Gene 173:33-38.
7. Desai, S. A., D. J. Krogstad, and E. W. McClesky. 1993. A nutrient-permeable channel on the intraerythrocytic malaria parasite. Nature 362:643-646.
8. Gennis, R. B. 1989. Biomembranes: molecular structure and function, p. 270-382. Springer-Verlag KG, Berlin, Germany.
9. Graessmann, M., and A. Graessmann. 1983. Microinjection of tissue culture cells. Methods Enzymol. 101:482-492.
10. Hackstadt, T., D. D. Rockey, R. A. Heinzen, and M. A. Scidmore. 1996. Chlamydia trachomatis interrupts an exocytic pathway to acquire endogenously synthesized sphingomyelin in transit from the Golgi apparatus to the plasma membrane. EMBO J. 15:964-977.
11. Hackstadt, T., M. A. Scidmore, and D. D. Rockey. 1995. Lipid metabolism in Chlamydia trachomatis infected cells: directed trafficking of Golgi-derived sphingolipids to the chlamydial inclusion. Proc. Natl. Acad. Sci. USA 92: 4877-4881.
12. Hatch, T. P. 1975. Utilization of L-cell nucleosidc triphosphates by Chlamydia psittaci for ribonucleic acid synthesis. J. Bacteriol. 122:393-400.
13. Hatch, T. P. 1975. Competition between Chlamydia psittaci and L cells for host isoleucine pools: a limiting factor in chlamydial multiplication. Infect. Immun. 12:211-220.
14. Heinzen, R. A., M. A. Scidmore, D. D. Rockey, and T. Hackstadt. 1996. The Chlamydia trachomatis inclusion is disconnected from the endocytic pathway, abstr. D-6, p. 242. Abstracts of the 96th General Meeting of the American Society tor Microbiology 1996. American Society for Microbiology, Washington. D.C.
15. Heinzen, R. A., M. A. Sddmore, D. D. Rockey, and T. Hackstadt. 1996. Differential interaction with endocytic and exocytic pathways distinguish parasitophorous vacuoles of Coxiella burnetii and Chlamydia trachomatis. Infect. Immun, 64:796-809.
16. Joiner, K. A., S. A. Fuhrman, H. M. Miettinen, L. H. Kasper, and I. Mellman. 1940. Toxoplasma gondii fusion competence of parasitophorous vacuoles in Fc receptor-transfected fibroblasts. Science 249:641-646.
17. Koval, M., and R. E. Pagano. 1991. Intracellular transport and metabolism of sphingomyelin. Biochim. Biophys. Acta 1082:113-125.
18. Kramer, M. J., and F. B. Gorden. 1971. Ultrastructural analysis of the effects of penicillin and chlortetracycline on the development of a genital tract Chlamydia. Infect. Immun. 3:333-341.
19. Manuël, J. 1996. Intracellular survival of protozoan parasites with special reference to Leishmania spp., Taxoplasma gondii and Trypanosoma cruzi. Adv. Parasitol. 38:1-51.
20. Matsumoto, A. 1988. Structural characteristics of chlamydial bodies, p. 21-46. In A. L. Barron (ed.). Microbiology of chlamydia. CRC Press, Inc., Boca Raton. Fla.
21. McClarty, G. 1994. Chlamydiae and the biochemistry of intracellular parasitism. Trends Microbiol. 2:157-164.
22. McClarty, G., und G. Tipples. 1991. In situ studies on incorporation of nucleic acid precursors into Chlamydia trachomatis DNA. J. Bacteriol. 173: 4922-4931.
23. Minaschek, G., J. Bereiter-Hahn, and G. Bertholdt. 1989. Quantitation of the volume of liquid injected into cells by means of pressure. Exp. Cell Res. 183:434-142.
24. Moulder, J. W. 1985. Comparative biology of intracellular parasitism. Microbiol. Rev. 49:298-337.
25. Moulder, J. W. 1991. Interaction of chlamydiae and host cells in vitro. Microbiol. Rev, 55:143-190.
26. Nichols, B. A., P. Y. Setzer, F. Pang, and C. R. Dawson. 1985. New view of the surface projections of Chlamydia trachomatis. J. Bacteriol. 164:344-349.
27. Paine, P. L., L. C. Moore, and S. B. Horowitz. 1975. Nuclear envelope permeability. Nature 254:109-114.
28. Pfeifer, U. 1987. Functional morphology of the lysosomal apparatus, p. 3-59. In H. Glaumann and F. J. Ballard (ed.), Lysosomes: their role in protein breakdown. Academic Press. London, England.
29. Rizzuto, R., M. Brini, P. Pizzo, M. Murgia, and T. Pozzan. 1995. Chimeric green fluorescent protein as a tool for visualizing subcellular organelles in living cells. Curr. Biol. 5:635-642.
30. Rockey, D. D., E. R. Fischer, and T. Hackstadt. 1996. Temporal analysis of the developing Chlamydia psittacia inclusion by use of fluorescence and electron microscopy. Infect. Immun. 64:4269-4278.
31. Rockey, D. D., R. A. Heinzen, and T. Hackstadt. 1995. Cloning and characterization of a Chlamydia psittaci gene coding for a protein localized in the inclusion membrane of infected cells. Mol. Microbiol, 15:617-626.
32. Sakin, H. 1995. Mechanosensitive channels. Annu. Rev. Physiol. 57:333-353.
33. Schwab, J. C., C. J. M. Beckers, and K. A. Joiner. 1994. The parasitophorous vacuole membrane surrounding intracellular Toxoplasma gondii functions as a molecular sieve, Proc. Natl. Acad. Sci. USA 91:509-513.
34. Scidmore, M. A., E. R. Fischer, and T. Hackstadt. 1996. Sphingolipids and glycoproteins are differentially trafficked to the Chlamydia trachomatis inclusion. J. Cell Biol. 134:363-374.
35. Scidmore, M. A., D. D. Rockey, E. R. Fischer, R. A. Heinzen, and T. Hackstadt. 1996. Vesicular interactions of the Chlamydia trachomatis inclusion are determined by chlamydial early protein synthesis rather than route of entry. Infect. Immun. 64:5366-5372.
36. Stochaj, U. and P. Silver. 1992. Nucleocytoplasmic traffic of proteins. Eur. J. Cell Biol. 59:1-11.
37. Tipples, G., and G. McClarty. 1993. The obligate intracellular bacterium Chlamydia trachomatis is auxotrophic for three of the four ribonuclcoside triphosphates. Mol. Microbiol. 8:1105-1114.
38. Todd, W. J., and H. D. Caldwell. 1985. The interaction of Chlamydia trachomatis with host cells: ultrastructural studies of the mechanism of release of a biovar II strain from HeLa 229 cells. J. Infect. Dis. 151:1037-1044.