DNA sequence of recombinase-binding sites can determine Xer site-specific recombination outcome

Molecular Microbiology

Volumn 23, Issue 2, 1997, Pages 387-398

  Blake, Jennifer A R ^a   Ganguly, Nivedita ^b   Sherratt, David J ^b  

^a UNIVERSITY OF DUNDEE   (United Kingdom)

^b UNIVERSITY OF OXFORD   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

CURVED DNA; RECOMBINASE;

ARTICLE; CELL DIVISION; CONTROLLED STUDY; DNA BINDING; DNA RECOMBINATION; DNA SEQUENCE; ESCHERICHIA COLI; NONHUMAN; PLASMID; PRIORITY JOURNAL; REPLICON;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0031033382     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2958.1997.2261600.x     Document Type: Article

References (21)

1. Bachmann, B.J. (1972) Pedigrees of some mutant strains of Escherichia coli K-12. Bacteriol Rev 36: 526-557.
2. Blakely, G., and Sherratt, D.J. (1996) Determinants of selectivity in Xer site-specific recombination. Genes Dev 10: 762-773.
3. Blakely, G., Colloms, S., May, G., Burke, M., and Sherratt, D.J. (1991) Escherichia coli XerC recombinase is required for chromosomal segregation at cell division. New Biologist 8: 789-798.
4. Blakely, G., May, G., McCulloch, R., Arciszewska, L.A., Burke, M., Lovett, S.T., and Sherratt, D.J. (1993) Two related recombinases are required for site-specific recombination at dif and cer in E. coli K-12. Cell 75: 351-361.
5. Blakely, G., Davidson, A.O., and Sherratt, D.J. (1997) Binding and cleavage of nicked substrates by site-specific recombinases XerC and XerD. J Mol Biol 265: 30-39.
6. Colloms, S.D., Sykora, P., Szatmari, G., and Sherratt, D.J. (1990) Recombination at ColE1 cer requires the Escherichia coli xerC gene product, a member of the Lambda integrase family of site-specific recombinases. J Bacteriol 172: 6973-6980.
7. Colloms, S.D., McCulloch, R., Grant, K., Neilson, L., and Sherratt, D.J. (1996) Xer-mediated site-specific recombination in vitro. EMBO J 15: 1172-1181.
8. Cornet, F., Mortier, I., Patte, J., and Louarn, J.-M. (1994) Plasmid pSC101 harbours a recombination site, psi, which is able to resolve plasmid multimers and to substitute for the analagous chromosomal Escherichia coli site dif. J Bacteriol 176: 3188-3195.
9. Kim, J., Zweib, C., Wu, C., and Adya, S. (1989) Bending of DNA by gene regulatory proteins: construction and use of a DNA bending vector. Gene 85: 15-23.
10. Kuempel, P.L., Henson, J.M., Dircks, M., Tecklenburg M., and Lim, D.F. (1991) dif, a recA-independent recombination site in the terminus region of the chromosome of Escherichia coli. New Biologist 3: 799-811.
11. Lane, D., Prentki, P., and Chandler, M. (1992) Use of gel retardation to analyse protein-nucleic acid interactions. Microbiol Rev 56: 509-528.
12. Leslie, N.R., and Sherratt, D.J. (1995) Site specific recombination in the replication terminus region of Escherichia coli: functional replacement of dif. EMBO J 14: 1561-1570.
13. Nunes-Duby, S.E., Matsumoto, L., and Landy, A. (1987) Site-specific recombination intermediates trapped with suicide substrates. Cell 50: 779-788.
14. Sambrook, J., Fritsch, E.F., and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press.
15. Sherratt, D.J., Arcisewska, L.K., Blakely, G., Colloms, S., Grant, K., Leslie, N., and McCulloch, R. (1995) Site-specific recombination and circular chromosome segregation. Phil Trans Soc Lond B 347: 37-42.
16. Stirling, C.J., Szatmari, G., Stewart, G., Smith, M.C.M., and Sherratt, D.J. (1988) The arginine repressor is essential for plasmid-stabilizing site-specific recombination at the ColE1 cer locus. EMBO J 7: 4389-4395.
17. Stirling, C.J., Colloms, S., Collins, J.F., Szatmari, G., and Sherratt, D.J. (1989) xerB, an Escherichia coli gene required for plasmid ColE1 site-specific recombination, is identical to pepA, encoding aminopeptidase A, a protein with substantial similarity to bovine lens leucine amino-peptidase. EMBO J 8: 1623-1627.
18. Summers, D.K. (1989) Derivatives of ColE1 cer show altered topological specificity in site-specific recombination. EMBO J 8: 309-315.
19. Summers, D.K., and Sherratt, D.J. (1984) Multimerization of high copy number plasmids causes instability: ColE1 encodes a determinant essential for plasmid monomerization and stability. Cell 36: 1097-1103.
20. Wu, H.M., and Crothers, D.M. (1984) The locus of sequence-directed and protein-induced DNA bending. Nature 308: 509-513.
21. Yanisch-Perron, C., Viera, J., and Messing, J. (1985) Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33: 103-119.