Dichloroacetate inhibits glutamine oxidation by decreasing pyruvate availability for transamination

Metabolism: Clinical and Experimental

Volumn 46, Issue 3, 1997, Pages 268-272

  Murray, Kevin ^a   Dickson, Alan J ^a  

^a UNIVERSITY OF MANCHESTER   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ALANINE; AMINOOXYACETIC ACID; DICHLOROACETIC ACID; GLUTAMINASE; GLUTAMINE; PYRUVIC ACID;

AMINO ACID SYNTHESIS; ARTICLE; CELL GROWTH; CONCENTRATION RESPONSE; CONTROLLED STUDY; GROWTH INHIBITION; HUMAN; HUMAN CELL; HYBRIDOMA; METABOLIC BALANCE; OXIDATION; PRIORITY JOURNAL; TRANSAMINATION;

EID: 0031027144     PISSN: 00260495     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0026-0495(97)90252-3     Document Type: Article

References (27)

1. Crabb DW, Yount EA, Harris RA: Metabolic effects of dichloroac-etate. Metabolism 30:1024-1039, 1981
2. Blackshear PJ, Holloway PAH, Alberti KGMM: Factors regulating amino acid release from extrasplanchnic tissues in the rat. Interactions of alanine and glutamine. Biochem J 150:379-387, 1975
3. Goodman MN, Ruderman NB, Aoki TT: Glucose and amino acid metabolism in perfused skeletal muscle. Effect of dichloroacetate. Diabetes 27:1065-1074, 1978
4. Whitehouse S, Cooper RH, Randle PJ: Mechanism of activation of pyruvate dehydrogenase by dichloroacetate and other halogenated carboxylic acids. Biochem J 141:761-774, 1974
5. Stacpoole PW, Greene YJ: Dichloroacetate. Diabetes Care 15:785-791, 1992
6. Jahoor F, Zhang XJ, Frazer E: Mechanism by which dichloroacetate lowers lactic acid levels: The kinetic interrelationships between lactate, pyruvate, alanine and glucose. Proc Soc Exp Biol Med 205:44-51, 1994
7. Murray K, Gull K, Dickson AJ: Dichloroacetate increases cell and antibody yields in batch cultures of a hybridoma cell line. Biotechnol Bioeng 49:377-382, 1996
8. Bettger WJ, McKeehan WL: Mechanisms of cellular nutrition. Physiol Rev 66:1-35, 1986
9. Knox WE, Horowitz ML, Friedell GH: Proportionality of glutaminase content to growth rate and morphology of neoplasms. Cancer Res 29:669-680, 1969
10. Horowitz ML, Knox WE, Morris HP: Glutaminase activity and growth rates of rat hepatomas. Cancer Res 29:1195-1199, 1969
11. Reitzer LJ, Wice BM, Kennel D: Evidence that glutamine, not sugar, is the major energy source for cultured HeLa cells. J Biol Chem 254:2669-2676, 1979
12. Patterson MK: Measurement of growth and viability of cells in culture. Methods Enzymol 58:141-152, 1979
13. Lamprecht W, Heinz F: Analysis of pyruvate using coupled enzyme systems, in Bergmeyer HU (ed): Methods of Enzymatic Analysis, vol 6. New York, NY, Academic, 1985, pp 570-577
14. Williamson DH: L-Alanine determination with alanine determination, in Bergmeyer HU (ed): Methods of Enzymatic Analysis, vol 8. New York, NY, Academic, 1985, pp 341-344
15. Jenkins HA, Butler M, Dickson AJ: Characterization of glutamine metabolism in two related murine hybridomas. J Biotechnol 23:167-182, 1992
16. Caughey WS, Smiley JD, Hellerman L: L-Glutamic acid dehydrogenase: Structural requirement for substrate competition, effects of thyroxine. J Biol Chem 224:591-607, 1956
17. Hopper S, Segal HL: Kinetic studies of rat liver glutaminealanine transaminase. J Biol Chem 237:3189-3195, 1962
18. Hornsby PJ, Gill GN: Regulation of glutamine and pyruvate oxidation in cultured cells by cortisol, antioxidants and oxygen: Effects on cell proliferation. J Cell Physiol 109:111-120, 1981
19. McAllister A, Allison SP, Randle PJ: Effects of dichloroacetate on the metabolism of glucose, pyruvate, acetate, 3-hydroxybutyrate and palmitate in rat diaphragm and heart muscle in vitro and on extraction of glucose, lactate, pyruvate and free fatty acids by dog heart in vivo. Biochem J 208:743-748, 1973
20. Shireman RB, Mace L, Davidson S: Effect of dichloroacetate and glyoxylate on low density lipoprotein uptake and on growth of cultured fibroblasts. Proc Soc Exp Biol Med 175:420-423, 1984
21. Dalili M, Ollos DF: Transient kinetics of hybridoma growth and monoclonal antibody production in serum-limited cultures. Biotechnol Bioeng 33:167-182, 1989
22. Ardawi MSM, Newsholme EA: Maximum activities of some enzymes of glycolysis, the tricarboxylic acid cycle and ketone body and glutamine utilisation pathways in lymphocytes of the rat. Biochem J 208:743-748, 1982
23. 15 N n.m.r. study of nitrogen metabolism in cultured mammalian cells. Biochem J 291:485-492, 1993
24. Goldstein L: Relation of glutamate to ammonia production in the rat kidney. Am J Physiol 210:661-666, 1966
25. Eagle H, Piez K: Population-dependent requirement by cultured mammalian cells for metabolites which they can synthesize. J Exp Med 116:29-43, 1962
26. Neuman RE, McCoy TA: Growth-promoting properties of pyruvate, oxaloacetate and α-ketoglutarate for isolated Walker carcinosarcoma 256 cells. Proc Soc Exp Biol Med 98:303-306, 1958
27. Ardawi MSM, Newsholme EA: Glutamine metabolism in lymphocytes of the rat. Biochem J 212:835-842, 1983