Transforming DNA integrates at multiple sites in the dimorphic fungal pathogen Blastomyces dermatitidis

Gene

Volumn 186, Issue 2, 1997, Pages 219-226

  Hogan, Laura H ^a,b   Klein, Bruce S ^a  

^a UNIVERSITY OF WISCONSIN SCHOOL OF MEDICINE AND PUBLIC HEALTH   (United States)

^b University of Wisconsin Health   (United States)

Author keywords

Chromosomal localization; Fungus; Hygromycin B resistance; Transcription initiation; Transformation; Virulence

Indexed keywords

EXTRACHROMOSOMAL DNA; FUNGAL DNA; HYGROMYCIN B;

ARTICLE; BLASTOMYCES DERMATITIDIS; BLASTOMYCOSIS; CHROMOSOME; CONTROLLED STUDY; DNA INTEGRATION; ELECTROPORATION; GENE EXPRESSION; GENE TRANSFER; GENETIC MANIPULATION; GENETIC TRANSFORMATION; MOLECULAR GENETICS; NONHUMAN; PATHOGENICITY; PLASMID; PRIORITY JOURNAL; PROMOTER REGION; SOUTHERN BLOTTING; TRANSCRIPTION INITIATION; VIRULENCE; YEAST;

ANIMALS; ASPERGILLUS; BASE SEQUENCE; BLASTOMYCES; CHROMOSOMES, FUNGAL; DNA, FUNGAL; DRUG RESISTANCE, MICROBIAL; ELECTROPORATION; ESCHERICHIA COLI; FUNGAL PROTEINS; GLUCAN 1,4-ALPHA-GLUCOSIDASE; GLYCOPROTEINS; HUMANS; HYGROMYCIN B; MAMMALS; MITOSIS; PHOSPHOTRANSFERASES (ALCOHOL GROUP ACCEPTOR); PLASMIDS; POLYMERASE CHAIN REACTION; PROMOTER REGIONS (GENETICS); RESTRICTION MAPPING; TRANSFORMATION, GENETIC; VIRULENCE;

AJELLOMYCES DERMATITIDIS; ASPERGILLUS; BLASTOMYCES; FUNGI; MAMMALIA;

EID: 0031020493     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1119(96)00713-5     Document Type: Article

References (25)

1. Al-Doory, Y. and DiSalvo, A.F. (1992) Blastomycosis. In: Greenough III, W.B. and Merigan, T.C. (Eds.), Current Topics in Infectious Disease. Plenum Medical, New York, pp. 268.
2. Bradsher, R.S. (1992) Blastomycosis. Clin. Infect. Dis. 14, S82-90.
3. Edman, J.C. (1992) Isolation of telomerelike sequences from Cryptococcus neoformans and their use in high-efficiency transformation. Mol. Cell. Biol. 12, 2777-2783.
4. Gurr, S.J., Unkles, S.E. and Kinghorn, J.R. (1987) The structure and organization of nuclear genes of filamentous fungi. In: Kinghorn, J.R. (Ed.), Gene Structure in Eukaryotic Microbes. IRL Press, Oxford, pp. 93-139.
5. Hogan, L.H., Josvai, S. and Klein, B.S. (1995) Genomic cloning, characterization, and functional analysis of the major surface adhesin WI-1 on Blastomyces dermatitidis yeasts. J. Biol. Chem. 270, 30725-30732.
6. Hogan, L.H., Klein, B.S. and Levitz, S.M. (1996) Virulence factors of medically important fungi. Clin. Microbiol. Rev. 9, in press.
7. Klein, B.S. and Jones, J.M. (1990) Isolation, purification and radiolabeling of a novel 120-kD surface protein on Blastomyces dermatitidis yeasts to detect antibody in infected patients. J. Clin. Invest. 85, 152-161.
8. Klein, B.S. and Jones, J.M. (1994) Purification and characterization of the major antigen WI-1 from Blastomyces dermatitidis yeasts and immunological comparison with A antigen. Infect. Immun. 62, 3890-3900.
9. Klein, B.S., Vergeront, J.M. and Davis, J.P. (1986) Epidemiologic aspects of blastomycosis, the enigmatic systemic mycosis. Semin. Respir. Infect. 1, 29-39.
10. Klein, B.S., Sondel, P.M. and Jones, J.M. (1992) WI-1, a novel 120-kilodalton surface protein on Blastomyces dermatitidis yeast cells, is a target antigen of cell-mediated immunity in human blastomycosis. Infect. Immun. 60, 4291-4300.
11. Klein, B.S., Hogan, L.H. and Jones, J.M. (1993) Immunological recognition of a 25-amino acid repeat arrayed in tandem on a major antigen of Blastomyces dermatitidis. J. Clin. Invest. 92, 330-337.
12. Klein, B.S., Chaturvedi, S., Hogan, L.H., Jones, J.M. and Newman, S.L. (1994) Altered expression of the surface protein WI-1 in genetically-related strains of Blastomyces dermatitidis that differ in virulence regulates recognition of yeasts by human macrophages. Infect. Immun. 62, 3536-3542.
13. Lodge, J.K., Jackson-Machelski, E., Toffaletti, D.L., Perfect, J.R. and Gordon, J.I. (1994) Targeted gene replacement demonstrates that myristoyl-CoA: protein N-myristoyltransferase is essential for viability of Cryptococcus neoformans. Proc. Natl. Acad. Sci. USA 91, 12008-12012.
14. Paris, S., Monod, M., Diaquin, M., Lamy, B., Arruda, L.K., Punt, P.J. and Latge, J.P. (1993) A transformant of Aspergillus fumigatus deficient in the antigenic cytotoxin ASPFI. FEMS Microbiol. Lett. 111, 31-36.
15. Perfect, J.R., Toffaletti, D.L. and Rude, T.H. (1993) The gene encoding phosphoribosylaminoimidazole carboxylase (ADE2) is essential for growth of Cryptococcus neoformans in cerebrospinal fluid. Infect. Immun. 61, 4446-4451.
16. Punt, P.J., Oliver, R.P., Dingemanse, M.A., Pouwels, P.H. and van den Hondel, C.A. (1987) Transformation of Aspergillus based on the hygromycin B resistance marker from Escherichia coli. Gene 56, 117-124.
17. Smith, J.M., Davies, J.E. and Holden, D.W. (1993) Construction and pathogenicity of Aspergillus fumigatus mutants that do not produce the ribotoxin restrictocin. Mol. Microbiol. 9, 1071-1077.
18. Smith, T.L., Gaskell, J., Berka, R.M., Yang, M., Henner, D.J. and Cullen, D. (1990) The promoter of the glucoamylase-encoding gene of Aspergillus niger functions in Ustilago maydis. Gene 88, 259-262.
19. Tang, C.M., Cohen, J. and Holden, D.W. (1992) An Aspergillus fumigatus alkaline protease mutant constructed by gene disruption is deficient in extracellular elastase activity. Mol. Microbiol. 6, 1663-1671.
20. Thau, N., Monod, M., Crestani, B., Rolland, C., Tronchin, G., Latge, J.P. and Paris, S. (1994) Rodletless mutants of Aspergillus fumigatus. Infect. Immun. 62, 4380-4388.
21. Woods, J.P. and Goldman, W.E. (1992) In vivo generation of linear plasmids with addition of telomeric sequences by Histoplasm capsulatum. Mol. Microbiol. 6, 3603-3610.
22. Woods, J.P. and Goldman, W.E. (1993) Autonomous replication of foreign DNA in Histoplasma capsulatum: role of native telomeric sequences. J. Bacteriol. 175, 636-641.
23. Woods, J.P. and Heinecke, E.L. (1996) Homologous gene targeting in Histoplasma capsulatum: URA5 gene disruption using a hygromycin resistance marker. Abstracts of the General Meeting of the American Society for Microbiology, Abstr. F116, p. 94.
24. Woods, J.P., Kersulyte, D., Goldman, W.E. and Berg, D.E. (1993) Fast DNA isolation from Histoplasma capsulatum: methodology for arbitrary primer polymerase chain reaction-based epidemiological and clinical studies. J. Clin. Microbiol. 31, 463-464.
25. Worsham, P.L. and Goldman, W.E. (1988) Quantitative plating of Histoplasma capsulatum without addition of conditioned medium or siderophores. J. Med. Vet. Mycol. 26, 137-143.