Cloning and characterization of tek, the gene encoding the major extracellular protein of Pseudomonas solanacearum

Molecular Plant-Microbe Interactions

Volumn 9, Issue 4, 1996, Pages 272-281

  Denny, Timothy P ^a   Ganova Raeva, Lilia M ^a   Huang, Jianzhong ^b   Schell, Mark A ^a,b  

^a UNIVERSITY OF GEORGIA   (United States)

^b UNIVERSITY OF GEORGIA   (United States)

Author keywords

Gene regulation; Protein export; Protein processing

Indexed keywords

PSEUDOMONAS; RALSTONIA SOLANACEARUM;

BACTERIAL DNA; BACTERIAL POLYSACCHARIDE; BACTERIAL PROTEIN; MEMBRANE PROTEIN; TEK PROTEIN, PSEUDOMONAS;

AMINO ACID SEQUENCE; ARTICLE; BACTERIAL GENE; BIOSYNTHESIS; GENETICS; MOLECULAR CLONING; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PATHOGENICITY; PSEUDOMONAS; VIRULENCE;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; BASE SEQUENCE; CLONING, MOLECULAR; DNA, BACTERIAL; GENES, BACTERIAL; MEMBRANE PROTEINS; MOLECULAR SEQUENCE DATA; POLYSACCHARIDES, BACTERIAL; PSEUDOMONAS; VIRULENCE;

EID: 0030138021     PISSN: 08940282     EISSN: None     Source Type: Journal    
DOI: 10.1094/MPMI-9-0272     Document Type: Article

References (45)

1. Altschul, S. F., Gish, W., Miller, W., E. W. Myers, and Lipman, D. J. 1990. Basic local alignment search tool. J. Mol. Biol. 215:403-410.
2. Arlat, M., VanGijsegem, F., Huet, J. C., Pernollet, J. C., and Boucher, C. A. 1994. PopA1, a protein which induces a hypersensitivity-like response on specific Petunia genotypes, is secreted via the Hrp pathway of Pseudomonas solanacearum. EMBO J. 13:543-553.
3. Ausubel, F. M., Brent, R., Kingston, R. E., More, D. D., Seidman, J. G., Smith, J. A., and Struhl, K. 1989. Short Protocols in Molecular Biology. Green Publishing Assoc. and Wiley-Interscience, New York.
4. Brumbley, S. M., Camey, B. F., and Denny, T. P. 1993. Phenotype conversion in Pseudomonas solanacearum due to spontaneous inactivation of PhcA, a putative LysR transcriptional activator. J. Bacteriol. 175:5477-5487.
5. Brumbley, S. M., and Denny, T. P. 1990. Cloning of phcA from wildtype Pseudomonas solanacearum, a gene that when mutated alters expression of multiple traits that contribute to virulence. J. Bacteriol. 172:5677-5685.
6. Carney, B. F., and Denny, T. P. 1990. A cloned avirulence gene from Pseudomonas solanacearum determines incompatibility on Nicotiana tabacum at the host species level. J. Bacteriol. 172:4836-4843.
7. Chou, P. Y., and Fasman, G. D. 1978. Prediction of the secondary structure of proteins from their amino acid sequence. Adv. Enzymol. 47: 45-147.
8. Clough, S. J., Schell, M. A., and Denny, T. P. 1994. Evidence for involvement of a volatile extracellular factor in Pseudomonas solanacearum virulence gene expression. Mol. Plant-Microbe Interact. 7: 621-630.
9. Collins, M. L., and Hunsaker, W. R. 1985. Improved hybridization assays employing tailed oligonucleotide probes: A direct comparison with 5′-end-labeled oligonucleotide probes and nick-translated plasmid probes. Anal. Biochem. 151:211-224.
10. Denny, T. P., and Baek, S. R. 1991. Genetic evidence that extracellular polysaccharide is a virulence factor of Pseudomonas solanacearum. Mol. Plant-Microbe Interact. 4:198-206.
11. Denny, T. P., Carney, B. F., and Schell, M. A. 1990. Inactivation of multiple virulence genes reduces the ability of Pseudomonas solanacearum to cause wilt symptoms. Mol. Plant-Microbe Interact. 3:293-300.
12. Denny, T. P. and Schell, M. A. 1994. Virulence and pathogenicity of Pseudomonas solanacearum: Genetic and biochemical perspectives. Pages 127-148 in: Biotechnology and Plant Protection: Bacterial Pathogenesis and Disease Resistance. D. Bills and S.-D. Kung, eds. World Scientific Publishing Co., River Edge, NJ.
13. Devereux, J., Haeberli, P., and Smithies, O. 1984. A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 12: 387-395.
14. Doolittle, R. F. 1986. Of URFs and ORFs: A primer on how to analyze derived amino acid sequences. University Science Books, Mill Valley, CA.
15. Galan, J. E., Ginocchio, C., and Costeas, P. 1992. Molecular and functional characterization of the Salmonella invasion gene invA: Homology of InvA to members of a new protein family. J. Bacteriol. 174: 4338-4349.
16. Gilchrist, A., Fisher, J. A., and Smit, J. 1992. Nucleotide sequence analysis of the gene encoding the Caulobacter crescentus parachystalline surface layer protein. Can. J. Microbiol. 38:193-202.
17. He, S. Y, Huang, H. C., and Collmer, A. 1993. Pseudomonas syringae pv. syringae harpin: A protein that is secreted via the Hrp pathway and elicits the hypersensitive response in plants. Cell 73:1255-1266.
18. Huang, J., Carney, B. F., Denny, T. P., Weissinger, A. K., and Schell, M. A. 1995. A complex network regulates expression of eps and other virulence genes of Pseudomonas solanacearum. J. Bacteriol. 177: 1259-1267.
19. Huang, J., Denny, T. P., and Schell, M. A. 1993. VsrB, a regulator of virulence genes in Pseudomonas solanacearum, is homologous to sensors of the two-component regulatory family. J. Bacteriol. 175: 6169-6178.
20. Huang, J., and Schell, M. A. 1990a. DNA sequence analysis of pglA and mechanism of export of its polygalacturonase product from Pseudomonas solanacearum. J. Bacteriol. 172:3879-3887.
21. Huang, J., and Schell, M. A. 1990b. Evidence that extracellular export of the endoglucanase encoded by egl of Pseudomonas solanacearum occurs by a two-step process involving a lipoprotein intermediate. J. Biol. Chem. 265:11628-11632.
22. Huang, J., and Schell, M. A. 1995. Molecular characterization of the eps gene cluster of Pseudomonas solanacearum and its transcriptional regulation at a single promoter. Mol. Microbiol. 16:977-989.
23. Huang, J., Sukordhaman, M., and Schell, M. A. 1989. Excretion of the egl gene product of Pseudomonas solanacearum. J. Bacteriol. 171: 3767-3774.
24. Hunkapiller, M. W., Lujan, E., Ostrander, F., and Hood, L. E. 1983. Isolation of microgram quantities of proteins from polyacrylamide gels for amino acid sequence analysis. Methods Enzymol. 91:227-236.
25. Kang, Y., Huang, J., Mao, G., He, L.-Y., and Schell, M. A. 1994. Dramatically reduced virulence of mutants of Pseudomonas solanacearum defective in export of extracellular proteins across the outer membrane. Mol. Plant-Microbe Interact. 7:370-377.
26. Kao, C. C., Barlow, E., and Sequeira, L. 1992. Extracellular polysaccharide is required for wild-type virulence of Pseudomonas solanacearum. J. Bacteriol. 174:1068-1071.
27. Kao, C. C., and Sequeira, L. 1991. A gene cluster required for coordinated biosynthesis of lipopolysaccharide and extracellular polysaccharide also affects virulence of Pseudomonas solanacearum. J. Bacteriol. 173:7841-7847.
28. Kao, C. C., and Sequeira, L. 1994. The function and regulation of genes required for extracellular polysaccharide synthesis and virulence in Pseudomonas solanacearum. Pages 93-108 in: Molecular Mechanism of Bacterial Virulence. C. I. Kado and J. H. Crosa, eds. Kluwer Academic Publishers, Dordrecht, The Netherlands.
29. Mead, D. A., Skorupa, E. S., and Kemper, B. 1985. Single stranded DNA promoter plasmids for engineering mutant RNA's and protein: Synthesis of a 'stretched' parathyroid hormone. Nucleic Acids Res. 13:1103-1108.
30. Miller, J. H. 1972. Experiments in Molecular Genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
31. Miyada, C. G., and Wallace, R. B. 1987. Oligonucleotide hybridization techniques. Methods Enzymol. 154:94-107.
32. Orgambide, G., Montrozier, H., Servin, P., Roussel, J., Trigalet-Demery, D., and Trigalet, A. 1991. High heterogeneity of the exopolysaccharides of Pseudomonas solanacearum strain GMI 1000 and the complete structure of the major polysaccharide. J. Biol. Chem. 266:8312-8321.
33. Pugsley, A. P., and Schwartz, M. 1985. Export and secretion of proteins by bacteria. FEMS Microbiol. Rev. 32:3-38.
34. Roberts, D. P., Denny, T. P., and Schell, M. A. 1988. Cloning of the egl gene of Pseudomonas solanacearum and analysis of its role in phytopathogenicity. J. Bacteriol. 170:1445-1451.
35. Salmond, G. P. C. 1994. Secretion of extracellular virulence factors by plant pathogenic bacteria. Annu. Rev. Phytopathol. 32:181-200.
36. Sambrook, J., Fritsch, E. F., and Maniatis, T. A. 1989. Molecular Cloning, A Laboratory Manual. 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
37. Schell, M. A. 1987. Purification and characterization of an endoglucanase from Pseudomonas solanacearum. Appl. Environ. Microbiol. 53: 2237-2241.
38. Schell, M. A., Denny, T. P., Clough, S. J., and Huang, J. 1993. Further characterization of genes encoding extracellular polysaccharide of Pseudomonas solanacearum and their regulation. Pages 231-239 in: Advances in Molecular Genetics of Plant-Microbe Interactions, Vol. 2. E. W. Nester and D. P. S. Verma, eds. Kluwer Academic Publishers, Dordrecht, The Netherlands.
39. Schell, M. A., Denny, T. P., and Huang, J. 1994a. Extracellular virulence factors of Pseudomonas solanacearum: Role in disease and their regulation. Pages 311-324 in: Molecular Mechanism of Bacterial Virulence. C. I. Kado and J. H. Crosa, eds. Kluwer Academic Publishers, Dordrecht, The Netherlands.
40. Schell, M. A., Denny, T. P., and Huang, J. 1994b. VsrA, a second two-component system regulating virulence genes of Pseudomonas solanacearum. Mol. Microbiol. 11:489-500.
41. Simon, R., Quandt, J., and Klipp, W. 1989. New derivatives of transposon Tn5 suitable for mobilization of replicons, generation Gene 80: fusions and induction of genes in Gram-negative bacteris. Gene 80: 161-169.
42. Staskawicz, B., Dahlbeck, D., Keen, N., and Napoli, C. 1987. Molecular characterization of cloned avirulence genes from race 0 and race 1 of Pseudomonas syringae pv. glycinea. J. Bacteriol. 169:5789-5794.
43. Van den Eede, G., Deblaere, R., Goethals, K., Van Montagu, M. and Holsters, M. 1992. Broad host range and promoter selection vectors for bacteria that interact with plants. Mol. Plant-Microbe Interact. 5: 228-234.
44. Wei, Z., Laby, R. J., Zumoff, C. H., Bauer, D. W., He, S. Y., Collmer, A., and Beer, S. V. 1992. Harpin, elicitor of the hypersensitive response produced by the plant pathogen Erwinia amyhvora. Science 257:85-88.
45. Wu, H. C., and Tokunaga, M. 1986. Biogenesis of lipoproteins in bacteria. Curr. Top. Microbiol. Immunol. 125:127-157.