Adenovirus-mediated gene transfer of dominant negative Ras(asn17) in 3T3L1 adipocytes does not alter insulin-stimulated PI3-kinase activity or glucose transport

Molecular Endocrinology

Volumn 11, Issue 1, 1997, Pages 67-76

  Gnudi, Luigi ^a,c   Frevert, Ernst U ^a   Houseknecht, Karen L ^a   Erhardt, Peter ^b   Kahn, Barbara B ^a  

^a BETH ISRAEL DEACONESS MEDICAL CENTER   (United States)

^b DANA FARBER CANCER INSTITUTE   (United States)

^c UNIVERSITY OF PADOVA   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

EPIDERMAL GROWTH FACTOR; INSULIN; MITOGEN ACTIVATED PROTEIN KINASE;

AMINO ACID SUBSTITUTION; ANIMAL CELL; ARTICLE; ENZYME ACTIVITY; GENE TRANSFER; GENETIC TRANSFECTION; GLUCOSE TRANSPORT; NONHUMAN; PHOSPHORYLATION; PRIORITY JOURNAL; SIGNAL TRANSDUCTION; TARGET CELL;

ADENOVIRIDAE; ANIMALIA;

EID: 0031014997     PISSN: 08888809     EISSN: None     Source Type: Journal    
DOI: 10.1210/mend.11.1.9866     Document Type: Article

References (35)

1. Cheatham B, Kahn CR 1995 Insulin action and the insulin signaling network. Endocr Rev 16:117-142
2. Kodaki T, Woscholski R, Hallberg B, Rodriguez-Viciana P, Downward J, Parker PJ 1994 The activation of phosphatidylinositol 3-kinase by Ras. Curr Biol 4:798-806
3. Viciana-Rodriguez P, Warne PH, Dhand R, Vanhaesebroeck B, Gout I, Fry MJ, Waterfield MD, Downward J 1994 Phosphatidylinositol-3-OH kinase as a direct target of Ras. Nature 370:527-532
4. Hu Q, Klippel A, Muslin AJ, Fantl WJ, Williams LT 1995 Ras-dependent induction of cellular responses by constitutively active phosphatidylinositol-3 kinase. Science 268:100-102
5. Sjolander A, Yamamoto K, Huber BE, Lapetina EG 1991 Association of p21 ras with phosphatidylinositol 3-kinase. Proc Natl Acad Sci USA 88:7908-7912
6. Rodriguez-Viciana P, Warne PH, Vanhaesebroeck B, Waterfield MD, Downward J 1996 Activation of phosphoinositide 3-kinase by interaction with Ras and by point mutation. EMBO J 15:2442-2451
7. Lazar DF, Wiese RJ, Brady MJ, Mastick CC, Waters SB, Yamauchi K, Pessin JE, Cuatrecasas P, Saltiel AR 1995 Mitogen-activated protein kinase kinase inhibition does not block the stimulation of glucose utilization by insulin. J Biol Chem 270:20801-20807
8. Kahn BB 1992 Facultative glucose transporters: regulatory mechanisms and dysregulation in diabetes. J Clin Invest 89:1367-1374
9. Quon MJ, Chen H, Ing BL, Liu ML, Zarnowski MJ, Yonezawa K, Kasuga M, Cushman SW, Taylor SI 1995 Roles of -1-Phosphatidylinositol 3-Kinase and ras in regulating translocation of GLUT4 in transfected rat adipose cells. Mol Cell Biol 15:5403-5411
10. Clarke J, Young P, Yonezawa K, Kasuga M, Holman G 1994 Inhibition of the translocation of GLUT1 and GLUT4 in 3T3-L1 cells by the phosphatidylinositol 3-kinase inhibitor, wortmannin. Biochem J 300:631-635
11. Cheatham B, Vlahos C, Cheatham L, Wang L, Blenis J, Kahn CR 1994 Phosphatidylinositol 3-kinase activation is required for insulin stimulation of pp70 S6 kinase, DNA synthesis, and glucose transporter translocation. Mol Cell Biol 14:4902-4911
12. Haruta T, Morris AJ, Rose DW, Nelson JG, Mueckler M, Olefsky JM 1995 Insulin-stimulated GLUT4 translocation is mediated by a divergent intracellular signaling pathway. J Biol Chem 270:27991-27994
13. Wiese RJ, Mastick CC, Lazar DF, Saltiel AR 1995 Activation of mitogen-activated protein kinase and phosphatidylinositol 3′-kinase is not sufficient for the hormonal stimulation of glucose uptake, lipogenesis, or glycogen synthesis is 3T3-L1 adipocytes. J Biol Chem 270:3442-3446
14. Navé BT, Haigh RJ, Hayward AC, Siddle K, Shepherd PR. Compartment-specific regulation of phosphoinositide 3-kinase by platelet-derived growth factor and insulin in 3T3-L1 adipocytes. Biochem J 313:55-60
15. Herbst JJ, Andrews GC, Contillo LG, Singleton DH, Genereux PE, Gibbs EM, Lienhard GE 1995 Effect of the activation of phosphatidylinositol 3-kinase by a thiophosphotyrosine peptide on glucose transport in 3T3L1 adipocytes. J Biol Chem 270:26000-26005
16. Hausdorff SF, Frangioni JV, Birnbaum MJ 1994 Role of p21ras in insulin-stimulated glucose transport in 3T3-L1 adipocytes. J Biol Chem 269:21391-21394
17. Fingar DC, Birnbaum MJ 1994 A role for Raf-1 in the divergent signaling pathways mediating insulin-stimulated glucose transport. J Biol Chem 269:10127-10132
18. Manchester J, Kong X, Lowry OH, Lawrence Jr JC 1994 Ras signaling in the activation of glucose transport by insulin. Proc Natl Acad Sci USA 91:4644-4648
19. Houseknecht KL, Zhu AX, Gnudi L, Hamann A, Zierath JR, Flier JS, Kahn BB 1996 Overexpression of H-Ras selectively in adipose tissue of transgenic mice: evidence for enhanced sensitivity to insulin. J Biol Chem 271:11347-11355
20. Kozma L, Baltensperger K, Klarlund J, Porras A, Santos E, Czech MP 1993 The Ras signaling pathway mimics insulin action on glucose transporter translocation. Proc Natl Acad Sci USA 90:4460-4464
21. Benito M, Porras A, Nebreda AR, Santos E 1991 Differentiation of 3T3L1 fibroblast to adipocytes induced by transfection of ras oncogenes. Science 253:565-568
22. Sale EM, Atkinson PGP, Sale GJ 1995 Requirement of Map kinase for differentiation of fibroblast to adipocytes, for insulin activation of p90 S6 kinase and for insulin or serum stimulation of DNA synthesis. EMBO J 14:674-684
23. Gnudi L, Zhu AX, Hamann A, Houseknecht KL, Flier JS, Kahn BB 1995 Obesity and divergent effects on insulin signaling in transgenic mice expressing a dominant negative mutant ras. Diabetes 44:20A
24. Cai H, Szeberenyi J, Cooper GM 1988 Inhibition of NIH 3T3 cell proliferation by a mutant Ras protein with preferential affinity for GDP. Mol Cell Biol 8:3235-3243
25. Dorrestijn J, Ouwens DM, Berghe N Vd, Bos JL, Maassen JA 1996 Expression of a dominant negative ras mutant does not affect stimulation of glucose uptake and glycogen synthesis by insulin. Diabetologia 39:558-563
26. DePaolo D, Reusch JEB, Carel K, Bhuripanyo P, Leitner JW, Draznin B 1996 Functional interactions of phosphatidylinositol 3-kinase with GTPase-activating protein in 3T3-L1 adipocytes. Mol Cell Biol 16:1450-1457
27. Becker TC, Noel RJ, Coats WS, Gomez-Foix AM, Alam T, Gerard RD, Newgard CB 1994 Use of recombinant adenovirus for metabolic engineering of mammalian cells. Methods Cell Biol 43:161-189
28. Gluzman Y, Reichl H, Solnic D 1982 Helper-free adenovirus type-5 vectors. In: Gluzman Y (ed) Eucaryotic Viral Vectors. Harbor Laboratory Press, Cold Spring Harbor, NY, pp 187-192
29. Shepherd PR, Nave BT, Siddle K 1995 Insulin stimulation of glycogen synthesis and glycogen synthase activity is blocked by wortmannin and rapamycin in 3T3L1 adipocytes: evidence for the involvement of phosphoinositide 3-kinase and p70 ribosomal protein-S6 kinse. Biochem J 305:25-28
30. Hill DP, Wurst W 1993 Screening for novel pattern formation genes using gene trap approaches. Methods Enzymol 225:664-681
31. Gibbs JB 1995 Determination of guanine nucleotide bound to Ras in mammalian cells. Methods Enzymol 255:118-125
32. Chang PY, Goodyear LJ, Benecke H, Markuns JS, Moller DE 1995 Impaired insulin signaling in skeletal muscles from transgenic mice expression kinase-deficient insulin receptors. J Biol Chem 270:12593-12600
33. Lamphere L, Carpenter CL, Sheng ZF, Kallen RG, Lienhard GE 1994 Activation of PI 3-kinase in 3T3L1 adipocytes by association with insulin receptor substrate-1. Am J Physiol 266:E486-E494
34. Kahn BB, Charron MJ, Lodish HF, Cushman SW, Flier JS 1989 Differential regulation of two glucose transporters in adipose cells from diabetic and insulin treated diabetic rats. J Clin Invest 84:404-411
35. Labarca C, Paigen K 1980 A simple rapid and sensitive DNA assay procedure. Ann Biochem Exp Med (Calcutta) 102:344-351