Complementation of temperature-sensitive topoisomerase II mutations in Saccharomyces cerevisiae by a human TOP2β construct allows the study of topoisomerase IIβ inhibitors in yeast

Cancer Chemotherapy and Pharmacology

Volumn 39, Issue 4, 1997, Pages 367-375

  Meczes, Emma L ^a   Marsh, Katherine L ^a   Fisher, L Mark ^b   Rogers, M Perenna ^a   Austin, Caroline A ^a  

^a NEWCASTLE UNIVERSITY   (United Kingdom)

^b ST GEORGE'S UNIVERSITY OF LONDON   (United Kingdom)

Author keywords

Doxorubicin; Etoposide; mAMSA; Mitoxantrone; Topoisomerase II

Indexed keywords

AMSACRINE; DNA TOPOISOMERASE (ATP HYDROLYSING); DOXORUBICIN; ETOPOSIDE; GENISTEIN; ISOENZYME; MERBARONE; MITOXANTRONE; QUERCETIN; SURAMIN;

ARTICLE; CELL KILLING; DRUG EFFECT; DRUG RESISTANCE; MUTATION; NONHUMAN; PRIORITY JOURNAL; SACCHAROMYCES CEREVISIAE; SHUTTLE VECTOR;

AMSACRINE; DNA TOPOISOMERASES, TYPE II; GENETIC COMPLEMENTATION TEST; GENETIC VECTORS; HUMANS; ISOENZYMES; MUTATION; SACCHAROMYCES CEREVISIAE; TEMPERATURE; TRANSFORMATION, GENETIC;

EID: 0031014248     PISSN: 03445704     EISSN: None     Source Type: Journal    
DOI: 10.1007/s002800050585     Document Type: Article

References (36)

1. Austin CA, Fisher LM (1990) Isolation and characterization of a human cDNA clone encoding a novel DNA topoisomerase II homologue from HeLa cells. FEBS Lett 266:115
2. Austin CA, Sng JH, Patel S, Fisher LM (1993) Novel HeLa topoisomerase II is the beta isoform: complete coding sequence and homology with other type II topoisomerases. Biochim Biophys Acta 1172:283
3. Austin CA, Marsh KL, Wasserman RA, Willmore E, Sayer PJ, Wang JC, Fisher LM (1995) Expression, domain structure and enzymatic properties of an active recombinant human DNA topoisomerase IIβ. J Biol Chem 270:15739
4. Bojanowski K, Lelievre S, Markovits J, Couprie J, Jacquemin Sablon A, Larsen AK (1992) Suramin is an inhibitor of DNA topoisomerase II in vitro and in Chinese hamster fibrosarcoma cells. Proc Natl Acad Sci USA 89:3025
5. Capranico G, Tinelli S, Austin CA, Fisher ML, Zunino F (1992) Different patterns of gene expression of topoisomerase II isoforms in differentiated tissues during murine development. Biochim Biophys Acta 1132:43
6. Chen M, Beck WT (1995) Differences in inhibition of chromosome separation and G2 arrest by DNA topoisomerase II inhibitors merbarone and VM-26. Cancer Res 55:1509
7. Chung TDY, Drake FH, Tan KB, Per SR, Crooke ST, Mirabelli CK (1989) Characetrization and immunological identification of cDNA clones encoding two human DNA topoisomerase II isoenzymes. Proc Natl Acad Sci USA 86:9431
8. Dereuddre S, Frey S, Delaporte C, Jacquemin Sablon A (1995) Cloning and characterization of full-length cDNAs coding for the DNA topoisomerase IIβ from Chinese hamster lung cells sensitive and resistant to 9-OH-ellipticine. Biochim Biophys Acta 1264:178
9. Drake FH, Hofmann GA, Mong S-M, O'Leary Bartus J, Hertzberg RP, Johnson RK, Mattern MR, Mirabelli CK (1989) In vitro and intracellular inhibition of topoisomerase II by the antitumor agent merbarone. Cancer Res 49:2578
10. Drake FH, Hofmann GA, Bartus HF, Mattern MR, Crooke ST, Mirabelli CK (1989) Biochemical and pharmacological properties of p170 and p180 forms of topoisomerase II. Biochemistry 28:8154
11. Eder JP Jr, Chane VTW, Niemierko E, Teicher BA, Schnipper LE (1993) Conditional expression of wild-type topoisomerase II complements a mutant enzyme in mammalian cells. J Biol Chem 268:13844
12. Harker WG, Slade DL, Drake FH, Parr RL (1991) Mitoxantrone resistance in HL-60 leukaemia cells: reduced nuclear topoisomerase II catalytic activity and drug-induced DNA cleavage in association with reduced expression of the topoisomerase IIβ isoform. Biochemistry 30:9953
13. Harker WG, Slade DL, Parr RL, Holguin MH (1995) Selective use of an alternative stop codon and polyadenylation signal within intron sequences leads to a truncated topoisomerase IIα messager RNA and protein in human HL-60 leukemia cells selected for resistance to mitoxantrone. Cancer Res 55:4962
14. Harker WG, Slade DL, Parr RL, Feldhoff PW, Sullivan DM, Holguin MH (1995) Alterations in the topoisomerase IIα gene, mRNA, and subcellular protein distribution as well as reduced expression of the DNA topoisomerase IIβ enzyme in a mitoxantrone-resistant HL-60 human leukaemia cell line. Cancer Res 55:1707
15. Hsiung Y, Elsea SH, Osheroff N, Nitiss JL (1995) A mutation in yeast TOP2 homologous to a quinolone-resistant mutation in bacteria. J Biol Chem 270:20359
16. Hsiung Y, Jannatipour M, Rose A, Mcmahon J, Duncan D, Nitiss JL (1996) Functional expression of human topoisomerase IIα in yeast: mutations at amino acids 450 or 803 of topoisomerase IIα result in enzymes that confer resistance to anti-topoisomerase II agents. Cancer Res 56:96
17. Ishida R, Miki T, Narita T, Yui R, Sato M, Utsumi KR, Tanabe K, Andoh T (1991) Inhibition of intracellular topoisomerase II by antitumour bis(2,6-dioxopiperazine) derivatives: mode of cell growth inhibition distinct from that of cleavable complex-forming type inhibitors. Cancer Res 51:4909
18. Ishida R, Hamatake M, Wasserman RA, Nitiss JL, Wang JC, Andoh T (1995) DNA topoisomerase II is the molecular target of bisdioxopiperazine derivatives ICRF-159 and ICRF-193 in Saccharomyces cerevisiae. Cancer Res 55:2299
19. Jannatipour M, Liu Y-X, Nitiss JL (1993) The top2-5 mutant of yeast topoisomerase II encodes an enzyme resistant to etoposide and amsacrine. J Biol Chem 268:18586
20. Liu Y-X, Hsiung Y, Jannatipour M, Yeh Y, Nitiss JL (1994) Yeast topoisomerase II mutants resistant to anti-topoisomerase agents: identification and characterisation of new yeast topoisomerase II mutants selected for resistance to etoposide. Cancer Res 54:2943
21. Marsh KL, Willmore E, Tinelli S, Cornarotti M, Meczes EL, Capranico G, Fisher LM, Austin CA (1996) Amsacrine promoted DNA cleavage site determinants for the two human DNA topoisomerase II isoforms, α and β. Biochemical Pharmacol (in press)
22. Nitiss JL (1994) Using yeast to study resistance to topoisomerase II-targeting drugs. Cancer Chemother Pharmacol 34:S6
23. Nitiss J, Wang JC (1988) DNA topoisomerase-targeting antitumor drugs can be studied in yeast. Proc Natl Acad Sci USA 85:7501
24. Nitiss JL, Liu Y-X, Harbury P, Jannatipour M, Wasserman RA, Wang JC (1992) Amsacrine and etoposide hypersensitivity of yeast cells overexpressing DNA topoisomerase II. Cancer Res 52:4467
25. Nitiss JL, Liu Y-X, Hsiung Y (1993) A temperature sensitive topoisomerase II allele confers temperature dependent drug resistance on amsacrine and etoposide: a genetic system for determining the targets of topoisomerase II inhibitors. Cancer Res 53:89
26. Petrov P, Drake FH, Loranger A, Huang W, Hancock R (1993) Localization of DNA topoisomerase II in Chinese hamster fibroblasts by confocal and electron microscopy. Exp Cell Res 204:73
27. Pommier Y, Leteurtre F, Fesen MR, Fujimori A, Bertrand R, Solary E, Kohlhagen G, Kohn KW (1994) Cellular determinants of sensitivity and resistance to DNA topoisomerase inhibitors. Cancer Invest 12:530
28. Roca J, Ishida R, Berger JM, Andoh T, Wang JC (1994) Antitumour bisdioxopiperazines inhibit yeast DNA topoisomerase II by trapping the enzyme in the form of a closed protein clamp. Proc Natl Acad Sci USA 91:1781
29. Ross WE (1985) DNA topoisomerases a targets for cancer therapy. Biochem Pharmacol 34:4191
30. Schneider E, Hsiang YH, Liu LF (1990) DNA topoisomerases as anticancer drug targets. Adv Pharmacol 21:149
31. Tanabe K, Ikegami Y, Ishida R, Andoh T (1991) Inhibition of topoisomerase II by antitumour agents bis(2,6-dioxopiperazine) derivatives. Cancer Res 51:4903
32. Tsai Pflugfelder M, Liu LF, Liu AA, Tewey KM, WhangPeng J, Knutsen T, Huebner K, Croce CM, Wang JC (1988) Cloning and sequencing of cDNA encoding human DNA topoisomerase II and localisation of the gene to chromosome region 17q21-22. Proc Natl Acad Sci USA 85:7177
33. Vassetzky YS, Alghisi G-C, Roberts E, Gasser S (1996) Ectopic expression of inactive forms of yeast DNA topoisomerase II confers resistance to the antitumour drug, etoposide. Br J Cancer 76:1201
34. Wang JC (1985) DNA topoisomerases. Annu Rev Biochem 54:665
35. Wasserman RA, Wang JC (1994) Analysis of yeast DNA topoisomerase II mutants resistant to the antitumor drug amsacrine. Cancer Res 54:1795
36. Wasserman RA, Austin CA, Fisher LM, Wang JC (1993) Use of yeast in the study of anticancer drugs targeting DNA topoisomerases: expression of a functional recombinant human DNA topoisomerase IIα in yeast. Cancer Res 53:3591