Functional expression of human topoisomerase IIα in yeast: Mutations at amino acids 450 or 803 of topoisomerase IIα result in enzymes that can confer resistance to anti-topoisomerase II agents

Cancer Research

Volumn 56, Issue 1, 1996, Pages 91-99

  Hsiung, Yuchu ^a,b   Jannatipour, Mehrdad ^a   Rose, Angela ^c   McMahon, Jeannette ^a   Duncan, Dennis ^a   Nitiss, John L ^a,b,c  

^a CHILDREN S HOSPITAL LOS ANGELES   (United States)

^b UNIVERSITY OF SOUTHERN CALIFORNIA   (United States)

^c ST JUDE CHILDREN S RESEARCH HOSPITAL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMSACRINE; DNA TOPOISOMERASE (ATP HYDROLYSING); ETOPOSIDE; GYRASE INHIBITOR;

AMINO ACID ANALYSIS; ANTINEOPLASTIC ACTIVITY; ARTICLE; CANCER INHIBITION; DRUG MECHANISM; DRUG RESISTANCE; ENZYME INHIBITION; GENE MUTATION; HUMAN; HUMAN CELL; PRIORITY JOURNAL; YEAST;

ALLELES; AMSACRINE; ANTINEOPLASTIC AGENTS; ARGININE; BASE SEQUENCE; DNA TOPOISOMERASES, TYPE II; DRUG RESISTANCE; HUMANS; MOLECULAR SEQUENCE DATA; POINT MUTATION; PROLINE; SACCHAROMYCES CEREVISIAE; TENIPOSIDE;

EID: 0030058786     PISSN: 00085472     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (44)

1. D'Arpa, P, and Liu, L. F. Topoisomerase-targeting antitumor drugs. Biochim Biophys Acta, 989: 163-177, 1989.
2. Beck, W T., and Danks, M. K. Mechanisms of resistance to drugs that inhibit DNA topoisomerases. Semin Cancer Biol., 2: 235-244, 1991.
3. Pommier, Y. DNA topoisomerase I and II in cancer chemotherapy: update and perspectives. Cancer Chemother. Pharmacol., 32: 103-108, 1993.
4. Roninson, I B. The molecular mechanism of multi-drug resistance in tumor cells. J Clin Physiol. Biochem., 5: 140-151, 1987.
5. Van der Bliek, A. M., and Borst, P Multidrug resistance. Adv. Cancer Res., 52: 165-203.
6. Heck, M M S., and Earnshaw, W E. Topoisomerase II a specific marker for cell proliferation. J. Cell Biol., 103: 2569-2581, 1986
7. Hsiang, Y., Wu, H-Y. and Liu, L. F. Proliferation-dependent regulation of DNA topoisomerase II in cultures of human cells. Cancer Res., 48: 3230-3235, 1988.
8. Heck, M M S , Hittelman, W. N., and Earnshaw, W. C Differential expression of DNA topoisomerases during the eukaryotic cell cycle. Proc. Natl. Acad. Sci USA, 85: 1086-1090, 1987.
9. Drake, F H., Hofmann, G. H., Mong, S-H., Bartus, J. O., Hertzberg, R. P., Johnson, R K , Mattern, M R., and Mirabelli, C. K. In vitro and intracellular inhibition of topoisomerase II by the antitumor agent merbarone. Cancer Res., 49: 2578-2583, 1989.
10. Woessner, R. D., Maltern, M. R., Mirabelli, C K., Johnson, R. K., and Drake, F H. Proliferation- and cell cycle-dependent differences in expression of the 170 kilodalton and 180 kilodalton forms of topoisomerase II in NIH-3T3 cells Cell Growth & Differ., 2: 209-214, 1991.
11. Zini, N., Martelli, A M, Sabatelli, P., Santi, S., Negri, C., Astaldi Ricotti, G. C. B and Maraldi, N. M. The 180-kDa isoform of topoisomerase II is localized in the nucleolus and belongs to the structural elements of the nucleolar remnant. Exp Cell Res., 200: 460-466, 1992.
12. Osheroff, N., and Corbett, A. When good enzymes go bad: conversion of topoisomerase II to a cellular toxin by anti-neoplastic drugs Chem Res. Toxicol., 6: 585-597, 1993.
13. Huang, W. Nucleotide sequences and the encoded amino acids of DNA topoisomerase genes. In: N. R. Cozzarelli and J. C. Wang (eds), DNA Topology and Its Biological Effects, pp. 265-284. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory, 1990
14. Caron, P. R., and Wang, J. C. DNA topoisomerases as targets of therapeutics: a structural overview In: T. Andoh, H. Ikeda, and M Ogura (eds.), Molecular Biology of DNA Topoisomerases and Its Applications to Chemotherapy, Proceedings of the International Symposium on DNA topoisomerases in Chemotherapy, Nagoya, Japan, 1991, pp 1-18. Boca Raton FL. CRC Press, 1991.
15. Lynn, R., Giaever, G , Goto, T., and Wang, J. C. Tandem regions of yeast DNA topoisomerase II share homology with different subunits of bacterial gyrase. Science (Washington DC), 233: 647-649, 1986.
16. Kato, J., Nishimura, Y., Imamura, R., Niki, H., Hiraga, S., and Suzuki. H. New topoisomerase essential for chromosome segregation in E. coli. Cell 63 393-404, 1990.
17. Wyckoff, E , and Hsieh, T Functional expression of a Drosophila gene in yeast. genetic complementation by DNA topoisomerase II. Proc Natl. Acad. Sci. USA, 85: 6272-6276, 1988.
18. Wasserman, R., Austin, C A., Fisher, L. M., and Wang, J. C Use of yeast in the study of anticancer drugs targeting DNA topoisomerase expression of a functional recombinant topoisomerase IIα in yeast. Cancer Res., 53: 3591-3596, 1993
19. Bugg, B., Danks, M. K., Beck, W. T , and Suttle, D P. Expression of a mutant DNA topoisomerase II in CCRF-CEM human leukemia cells selected for resistance to teniposide. Proc Natl. Acad. Sci USA, 88: 7654-7658, 1991.
20. Danks, M K., Schmidt, C. A , Citrain, M. C., Suttle, D. P., and Beck, W T. Altered catalytic activity of and DNA cleavage by DNA topoisomerase II from human leukemic cells selected for resistance to VM-26 Biochemistry, 27: 8861-8869, 1988.
21. r 170,000 isozyme of DNA topoisomerase II in human tumor cells Cancer Res., 53: 1373-1379, 1993.
22. Hinds, M., Deisseroth, K , Mayes, J., Altschuler, E., Jansen, R., Ledley, F , and Zwelling, L. A. Identification of a point mutation in the topoisomerase II gene from a human leukemia cell line containing an amsacrine-resistant form of topoisomerase II. Cancer Res , 51: 4729-4731, 1991.
23. Lee, M-S., Wang, J. C., and Beran, M Two amsacrine-resistant human leukemia cell lines share a common mutation in the TOP2 gene encoding the 170 kD form of DNA topoisomerase II J. Mol. Biol., 223: 837-843, 1992.
24. Patel S., and Fisher, L M. Novel selection and genetic characterization of an etoposide-resistant human leukemic CCRF-CEM cell line. Br J. Cancer, 67: 456-463, 1993
25. Nitiss, J., and Wang, J C. DNA topoisomerase-targeting anti-tumor drugs can be studied in yeast Proc. Natl. Acad. Sci. USA, 55: 7501-7505, 1988.
26. Nitiss, J. L., Liu, X-Y., Harbury, P., Jannatipour, M , Wasserman, R., and Wang, J C Amsacrine and etoposide hypersensitivity of yeast cells overexpressing DNA topoisomerase II. Cancer Res , 52 4467-4472, 1992
27. Nitiss, J. L., Liu, Y-X , and Hsiung, Y. A temperature sensitive topoisomerase II allele confers temperature dependent drug resistance to amsacrine and etoposide. a genetic system for determining the targets of topoisomerase II inhibitors. Cancer Res , 53: 89-93, 1993.
28. Liu, Y-X , Hsiung, Y , Jannatipour, M , Yen, Y., and Nitiss, J. L. Yeast topoisomerase II mutants resistant to anti-topoisomerase agents: identification and characterization of new yeast topoisomerase II mutants resistant to amsacrine and etoposide Cancer Res., 54: 2943-2951, 1994
29. Nitiss, J L., Vilalta, P. M., Wu, H., and McMahon, J. Mutations in the gyrB domain of eukaryotic topoisomerase II can lead to partially dominant resistance to etoposide and amsacrine. Mol. Pharmacol., 46: 773-777, 1994.
30. Jannaupour, M., Liu, Y-X., and Nitiss, J. L. The top2-5 mutant of yeast topoisomerase II encodes an enzyme resistant to etoposide and amsacrine J. Biol. Chem., 268: 18586-18592, 1993.
31. Schiestl, R. H., and Gietz, R. D. High efficiency transformation of intact yeast cells using single stranded nucleic acids as a carrier. Curr Genet., 16: 339-346, 1989.
32. Tsai-Phlugfelder, M., Liu, L. F., Liu, A. A., Tewey, K. M., Whang-Peng, J., Knutsen, T , Huebner, K., Croce, C. M., and Wang, J. C. Cloning and sequencing of cDNA encoding human topoisomerase II and localization of the gene to chromosome region 17q21-22. Proc. Natl. Acad. Sci. USA, 85: 7177-7181, 1988
33. Bradford, M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal Biochem , 72: 248-254, 1976.
34. Morel, C , Chiari, E., Mattel, D. M., Romanha, H. A., and Simpson, L Strains and clones of Trypanosma cruzi can be characterized by pattern of restriction endonuclease products of kinetoplast DNA minicircles. Proc. Natl. Acad. Sci. USA, 77: 6810-6814, 1980.
35. Sherman, F., Fink, G R , and Hicks, J B. Methods in Yeast Genetics. Cold Spring Harbor. NY: Cold Spring Harbor Laboratory Press, 1979.
36. Harlow, E., and Lane, D. Antibodies: A Laboratory Manual. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press, 1988.
37. Laemmli, U. K Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (Lond.), 227: 680-685, 1970.
38. Holm, C , Goto, T., Wang, J. C , and Botstein, D. DNA topoisomerase II is required at the time of mitosis in yeast. Cell, 41: 553-563, 1985.
39. Goto, T., and Wang, J. C., Yeast DNA topoisomerase II is encoded by a single-copy, essential gene Cell, 36: 1073-1080, 1984.
40. DiNardo, S., Voelkel, K., and Sterngtanz, R. DNA topoisomerase II mutant of Saccharomyces cerevisiae topoisomerase II is required for the segregation of daughter molecules at the termination of DNA replication. Proc. Natl. Acad. Sci. USA, 81: 2616-2620, 1984.
41. Thrash, K., Bankier, A., Barrell, B , and Sternglanz, R. Cloning, characterization, and sequencing of the yeast DNA topoisomerase I gene. Proc Natl. Acad. Sci. USA, 82: 4374-4378, 1985.
42. Goto, T , and Wang, J. C. Cloning of yeast TOP1, the gene DNA encoding DNA topoisomerase I, and construction of mutants defective in both DNA topoisomerase I and topoisomerase E. Proc. Natl Acad. Sci USA, 82: 7178-7182, 1985.
43. Brill, S. J., DiNardo, D., Voelkel-Meiman, K., and Sternglanz, R Need for DNA topoisomerase activity as a swivel for DNA replication and for transcription of ribosomal RNA. Nature (Lond.), 326: 414-416, 1987.
44. Guild, B. C., Mulligan, R. C., Gros, P., and Housman, D. E. Retroviral transfer of a murine cDNA for drug resistance confers pleiotropic resistance to cells without prior drug selection. Proc. Natl. Acad. Sci. USA, 85: 1595-1599, 1988.