Characterization of membrane-associated Pseudomonas aeruginosa Ras-like protein Pra, a GTP-binding protein that forms complexes with truncated nucleoside diphosphate kinase and pyruvate kinase to modulate GTP synthesis

Journal of Bacteriology

Volumn 179, Issue 7, 1997, Pages 2181-2188

  Chopade, Balu A ^a   Shankar, Sandeep ^a   Sundin, George W ^a   Mukhopadhyay, Subhendu ^a   Chakrabarty, A M ^a  

^a UNIVERSITY OF ILLINOIS   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GUANINE NUCLEOTIDE BINDING PROTEIN; GUANOSINE TRIPHOSPHATE; MEMBRANE PROTEIN; NUCLEOSIDE DIPHOSPHATE KINASE; PYRUVATE KINASE;

AMINO TERMINAL SEQUENCE; ARTICLE; AUTOPHOSPHORYLATION; CELL ADHESION; CELL SURVIVAL; COMPLEX FORMATION; ENZYME ACTIVITY; ENZYME SYNTHESIS; GENE EXPRESSION REGULATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTO ONCOGENE; PSEUDOMONAS AERUGINOSA;

ESCHERICHIA COLI; PSEUDOMONAS; PSEUDOMONAS AERUGINOSA;

EID: 0031004328     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.179.7.2181-2188.1997     Document Type: Article

References (38)

1. Ahn, J., P. E. March, H. E. Takiff, and M. Inouye. 1986. A GTP binding protein of Escherichia coli has homology to yeast Ras proteins. Proc. Natl. Acad. Sci. USA 83:1172-1176.
2. Almaula, N., Q. Lu, J. Delgado, S. Belkin, and M. Inouye. 1995. Nucleoside diphosphate kinase from Escherichia coli. J. Bacteriol. 177:2524-2529.
3. Barbacid, M. 1987. Ras genes. Annu. Rev. Biochem. 56:779-827.
4. Bowman, B. J., F. Blasco, and S. W. Slayman. 1981. Purification and characterization of the plasma membrane ATPasc of Neurospora crassa. J. Biol. Chem. 256:12343-12349.
5. Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-254.
6. Brock, H. W. 1987. Sequence and genomic structure of ras homologues dm-ras 85d and dm-ras 64h of Drosophila melanogaster. Gene 51:129-138.
7. Carlier, M. F. 1982. Guanosine-5′ triphosphate hydrolysis and tubulin polymerization. Mol. Cell. Biochem. 47:97-113.
8. Cashel, M. 1994. Detection of (p)ppGpp accumulation patterns in Escherichia coli mutants. Methods Mol. Genet. 3:341-356.
9. Cassel, D., and Z. Selinger. 1977. Mechanism of adenylate cyclase activation by cholera toxin: inhibition of GTP hydrolysis at the regulatory site. Proc. Natl. Acad. Sci. USA 74:3307-3311.
10. Chen, S.-M., H. E. Takiff, A. M. Barber, G. C. Dubois, J. C. A. Bardwell, and D. L. Court. 1990. Expression and characterization of RNase III and Era proteins. J. Biol. Chem. 265:2888-2895.
11. Darzins, A., and A. M. Chakrabarty. 1984. Cloning of genes controlling alginate biosynthesis from a mucoid cystic fibrosis isolate of Pseudomonas aeruginosa. J. Bacteriol. 159:9-18.
12. Deschenes, R. J., and J. R. Broach. 1987. Fatty acylation is important but not essential for Saccharomyces cerevisiae Ras function. Mol. Cell. Biol. 7:2344-2355.
13. Fukui, Y., T. Kazasa, Y. Kaziro, T. Takeda, and M. Yamamoto. 1986. Role of a ras homolog in the life cycle of Schizosaccharomyces pombe. Cell 44:329-336.
14. Fukui, Y. Y., Y. Kaziro, and M. Yamamoto. 1986. Mating pheromone like diffusible factor released by Schizosaccharomyces pombe, EMBO J. 5:1991-1993.
15. Gentry, D. R., V. J. Hernandez, L. H. Nguyen, D. B. Jensen, and M. Cashel. 1993. Synthesis of the stationary-phase sigma factor as is positively regulated by ppGpp. J. Bacteriol. 175:7982-7989.
16. Gibbs, J. B., and M. K. Marshall. 1989. The ras oncogene: an important regulatory element in lower eucaryotic organisms. Microbiol. Rev. 53:171-175.
17. Gilman, A. G. 1987. G-proteins: transducers of receptor induced signals. Annu. Rev. Biochem. 56:615-649.
18. Hughes, S. M. 1983. Are guanine nucleotidc binding proteins a distinct class of regulatory proteins? FEBS Lett. 164:1-8.
19. Kataoka, T., S. Powers, S. Cameron, O. Fasano, J. Strathern, J. Broach, and M. Wigler. 1984. Genetic analysis of yeast Saccharomyces cerevisiae ras1 and ras2 genes. Cell 37:437-446.
20. Lerner, C. G., and M. Inouye. 1991. Pleiotropic changes resulting from depletion of Era, an essential GTP-binding protein in Escherichia coli. Mol. Microbiol. 5:951-957.
21. Lin, Y., J. D. Sharer, and P. E. March. 1994. GTPase dependent signalling in bacteria: characterization of a membrane binding site for Era in Escherichia coli. J. Bacteriol. 176:44-49.
22. Lowy, D. R., and B. M. Williamson. 1993. Function and regulation of ras. Annu. Rev. Biochem. 62:851-891.
23. March, P. E. 1992. Membrane associated GTPases in bacteria. Mol. Microbiol. 6:1253-1257.
24. March, P. E., C. G. Lerner, J. Ahn, X. Cui, and M. Inouye. 1988. The Escherichia coli ras like protein Era has GTPase activity and is essential for cell growth. Oncogene 2:539-544.
25. May, T. B., and A. M. Chakrabarty. 1994. Pseudomonas aeruginosa: genes and enzymes of alginate biosynthesis. Trends Microbiol. 2:151-157.
26. Moore, P. B. 1995. Molecular mimicry in protein synthesis? Science 270: 1453-1454.
27. Mozer, B., R. Marlor, S. Parkhurst, and V. Corces. 1985. Characterization and developmental expression of a Drosophila melanogaster ras oncogene. Mol. Cell. Biol. 5:885-889.
28. Pillutla, R. C., J. D. Sharer, P. S. Gulati, S. Wu, Y. Yamashita, C. G. Lerner, M. Inouye, and P. E. March. 1995. Cross-species complementation of the indispensable Escherichia coli era gene highlights amino acid regions essential for activity. J. Bacteriol. 177:2194-2196.
29. Shankar, S., S. Kamath, and A. M. Chakrabarty. 1996. Two forms of the nucleoside diphosphate kinase of Pseudomonas aeruginosa 8830: altered specificity of nucleoside triphosphate synthesis by the cell membrane associated form of the truncated enzvme. J. Bacteriol. 178:1777-1781.
30. Stryer, L., J. B. Hurley, and B. K. K. Fung. 1981. Transducin: an amplifier protein in vision. Trends Biochem. Sci. 6:245-247.
31. Sundin, G. W., S. Shankar, S. A. Chughani, B. A. Chopade, A. Kavanaugh-Black, and A. M. Chakrabarty. 1996. Nucleoside diphosphate kinase from Pseudomonas aeruginosa: characterization of the gene and its role in cellular growth and exopolysaccharide alginate synthesis. Mol. Microbiol. 20:965-979.
32. 31a.Sundin, G. W., S. Shankar, and A. M. Chakrabarty. 1996. Mutational analysis of nucleoside diphosphate kinase from Pseudomonas aeruginosa: characterization of critical amino acid residues involved in exopolysaccharide alginate synthesis. J. Bacteriol. 178:7120-7128.
33. Trach, K., and J. A. Hoch. 1989, The Bacillus subtilis spo0B stage O sporulation operon encodes an essential GTP binding protein. J. Bacteriol. 171: 1362-1371.
34. Van Haastert, P. J. M., F. Kesbeke, C. D. Reymond, R. A. Firtel, E. Luderus, and R. van Driel. 1987. Aberrant transmembrane signal transduction in Dictyostelium cells expressing a mutated ras gene. Proc. Natl. Acad. Sci. USA 84:4905-4909.
35. Wang, X., and J. Lutkenhaus. 1993. The FtsZ protein of Bacillus subtilis is localized at the division site and has GTPase activity that is dependent upon FtsZ concentration. Mol. Microbiol. 9:435-442.
36. Welsh, K. M., K. A. Trach, C. Folger, and J. A. Hoch. 1994. Biochemical characterization of the essential GTP binding protein Obg of Bacillus subtilis. J. Bacteriol. 176:7161-7168.
37. Xie, Z.-D., C. D. Hershberger, S. Shankar, R. W. Ye, and A. M. Chakrabarty. 1996. Sigma factor-anti-sigma factor interaction in alginate synthesis: inhibition of AlgT by MucA. J. Bacteriol. 178:4990-4996.
38. Zuber, M., T. A. Hoover, B. J. Powell, and D. L. Court. 1994. Analysis of the rnc locus of Coxiella burnetti. Mol. Microbiol. 14:291-300.