Mechanical continuity and reversible chromosome disassembly within intact genomes removed from living cells

Journal of Cellular Biochemistry

Volumn 65, Issue 1, 1997, Pages 114-130

  Maniotis, Andrew J ^a   Bojanowski, Krzysztof ^a   Ingber, Donald E ^a  

^a BOSTON CHILDREN S HOSPITAL   (United States)

Author keywords

chromatin; histone; micromanipulation; mitosis; nuclear matrix; nucleolus; tensegrity

Indexed keywords

DEOXYRIBONUCLEASE; DNA; FORMAMIDE; GELSOLIN; HEPARIN; HISTONE; NUCLEASE; RIBONUCLEASE;

ANIMAL CELL; ARTICLE; CATTLE; CELL NUCLEUS MATRIX; CHROMATIN CONDENSATION; CHROMATIN STRUCTURE; CHROMOSOME CONDENSATION; CHROMOSOME STRUCTURE; CONTROLLED STUDY; ENDOTHELIUM CELL; GENOME; HUMAN; HUMAN CELL; INTERPHASE; METAPHASE; MICROMANIPULATION; MITOSIS; NONHUMAN; NUCLEOLUS; PRIORITY JOURNAL;

NASA DISCIPLINE CELL BIOLOGY; NON-NASA CENTER;

ANIMALS; CATTLE; CELL NUCLEUS; CHROMOSOMES; ENDOTHELIUM, VASCULAR; HISTONES; HUMANS; MOVEMENT; VIDEO RECORDING;

ANIMALIA; BOS TAURUS;

EID: 0030987115     PISSN: 07302312     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-4644(199704)65:1<114::AID-JCB12>3.0.CO;2-K     Document Type: Article

References (60)

1. Alexander SP, Rieder CL (1991): Kinetochores are transported poleward along a single astral microtubule during chromosome attachment to the spindle in newt lung cells. J Cell Biol 110:81-95.
2. Belmont A, Bruce K (1994): Visualization of G1 chromosomes: A folded, twisted supercoiled chromonema model of interphase chromatin structure. J Cell Biol 127:287-302.
3. Berezney R, Coffey DS (1975): Nuclear protein matrix: Association with newly synthesized DNA. Science 189: 291-292.
4. Bickmore WA, Oghene K (1996): Visualizing the spatial relationships between defined DNA sequences and the axial region of extracted metaphase chromosomes. Cell 84:95-104.
5. Burdick AB (1976): Somatic cell chromosome interconnections in trypan preparations of Chinese hamster testicular cells. Exp Cell Res 99:426-428.
6. Capco DS, Wan KM, Penman S (1982): The nuclear matrix: Three dimensional architecture and protein composition. Cell 29:847-858.
7. Cluzel P, Lebrun A, Heller C, Lavery R, Viovy JL, Chatanay D, Caron F (1996): DNA: An extensible molecule. Science 271:792-794.
8. Diacumakos E, Holland S, Pecora P (1971): Chromosome displacement in and extraction from human cells at different mitotic stages. Nature 232:33-236.
9. DuPraw E (1965): The organization of nuclei and chromosomes in honeybee embryonic cells. Proc Natl Acad Sci USA 53:161-168.
10. Earnshaw WC, Halligan B, Cooke CA, Heck MM, Liu LF (1985): Topoisomerase II is a structural component of mitotic chromosome scaffolds. J Cell Biol 100:1706-1715.
11. Fey EG, Wan KM, Penman S (1984): Epithelial cytoskeletal framework and nuclear matrix-intermediate filament scaffold: three dimensional organization and protein composition. J Cell Biol 98:1973-1984.
12. Forer A (1966): Characterization of the mitotic traction system, and evidence that birefiringent spindle fibers neither produce nor transmit force for chromosome movement. Chromosoma 19:44-98.
13. Garcia-Blanco MA, Miller DD, Sheetz MP (1995): Nuclear Spreads: Visualization of Bipartite Ribosomal Domains. J Cell Biol 128:15-27.
14. Gasser SM, Laroche T, Falquet J, Boy de la Tour E, Laemmli UK (1986): Metaphase chromosome structure: Involvement of topoisomerase II. J Mol Biol 188:613-629.
15. Hacques MF, Muller S, De Murcia G, Van Regenmortel MH, Marion C (1990): Accessibility and structural role of histone domains in chromatin: Biophysical and immunochemical studies of progressive digestion with immobilized proteases. J Biomol Struc Dynam 8:619-614.
16. Henderson E, Hardin CC, Walk SK, Ignacio Tinoco Jr, Blackburn EH (1987): Telomeric DNA oligonucleotides form novel intramolecular structures containing guanine-aguanine base pairs. Cell 51:899-908.
17. Hildebrand CE, Gurley LR, Tobey R, Walters RA (1977): Action of heparin on mammalian nuclei: cell-cycle specific changes in chromatin organization correlate temporally with histone H1 phosphorylation. Biochim Biophys Acta 517:295-311.
18. Hoskins GC (1965): Electron microscopic observations of human chromosomes isolated by micrurgy. Nature 207: 1215-1216.
19. Hoskins GC (1968): Sensitivity of micrurgically removed chromosomal spindle fibres to enzyme disruption. Nature 217:748-750.
20. Ingber DE (1990): Fibronectin controls capillary endothelial cell growth by modulating cell shape. Proc Natl Acad Sci USA 87:3579-3583.
21. Ingber DE, Dike L, Hansen L, Karp S, Liley H, Maniotis A, McNamee H, Mooney D, Plopper G, Sims J, Wang N (1994): Cellular tensegrity: Exploring how mechanical changes in the cytoskeleton regulate cell growth, migration, and tissue pattern during morphogenesis. Int Rev Cytol 150:173-224.
22. Jackson DA, Cook PR (1985): Transcription occurs at the nucleoskeleton. EMBO J 4:919-925.
23. Kas E, Poljak L, Adachi Y, Laemmli U (1993): A model for chromatin opening: Stimulation of topoisomerase II and restriction enzyme cleavage of chromatin by distamycin. EMBO J 12:115-26.
24. Kavenoff R, Zimm BH (1973): Chromosome-sized DNA molecules from Drosophila. Chromosoma 41:1-27.
25. Korf BR, Diacumakos E (1977): Random arrangement of mitotic chromosomes in radial metaphases of the Indian muntjac. Cytogenet Cell Genet 19:335.
26. Korf B, Diacumakos E (1980): Absence of true interchromosomal connectives in microsurgically isolated chromosomes. Exp Cell Res 130:377-385.
27. Li X, Nicklaus B (1995): Mitotic forces control a cell-cycle checkpoint. Nature 373:630-632.
28. Lintilhac PM, Vesecky TB (1984): Stress-induced alignment of division plane in plant tissues grown in vitro. Nature 307:363-364.
29. Mancini M, He D, Ouspenski I, Brinkley BR (1996): Dynamic continuity of nuclear and mitotic matrix proteins in the cell cycle. J Cell Biochem 62:158-164.
30. Maniotis A, Chen C, Ingber DE (1997): Demonstration of mechanical connections between integrins, cytoskeletal filaments and nucleoplasm that stabilize nuclear structure. Proc Natl Acad Sci USA 94:849-854.
31. Manuelidis L (1990): A view of interphase chromosomes. Science 250:1533-1540.
32. Maro B, Johnson MH, Webb M, Flach G (1986): Mechanism of polar body formation in the mouse oocyte: an interaction between the chromosomes, the cytoskeleton and the plasma membrane. J Embryol Exp Morphol 92:11-32.
33. Mazia D (1961): Mitosis and the physiology of cell division. In Brachet J, Mirsky A (eds): "The Cell." New York and London: Academic Press.
34. Miller OJ, Breg WR, Mukherjee BB, Gamble A (1963a): Nonrandom distribution of chromosomes in metaphase figures from cultured human leukocytes. 1 The peripheral location of chromosomes 13, 17-18, and 21. Cytogenetics 2:152-167.
35. Miller OJ, Mukherjee BB, Breg WR, Gamble A (1963b): Nonrandom distribution of chromosomes in metaphase figures from cultured human leukocytes. The peripheral location of the Y chromosome. Cytogenetics 2:1-14.
36. Mirkovitch J, Mirault ME, Laemmli UK (1984): Organization of the higher-order chromatin loop: Specific DNA attachment sites on nuclear scaffold. Cell 39:223-232.
37. Mitchison T, Evans L, Schulze E, Kirschner M (1986): Sites of microtubule assembly and disassembly in the mitotic spindle. Cell 45:515-527.
38. Myhra S, Brogger A (1975): Interchromosomal DNA-containing fibers in human cells. Human Genetic 29:183-189.
39. Nagele R, Freeman T, McMorrow L, Lee H (1995): Precise spacial positioning of chromosomes during prometaphase: Evidence for chromosomal order. Science 270: 1831-1835.
40. Nickerson JA, Krockmalnic G, Wan KM, Turner CD, Penman S (1992): A normally masked nuclear matrix antigen that appears at mitosis on cytoskeleton filaments adjoining chromosomes, centrioles, and midbodies. J Cell Biol 116:977-987.
41. Nicklas RB (1967): Chromosome micromanipulation: induced reorientation and the experimental control of segregation in meiosis. Chromosoma 21:17-50.
42. Nicklas RB, Kubai DF (1985): Microtubules, chromosome movement, and reorientation after chromosomes are dettached from the spindle by micromanipulation. Chromosoma 92:313-324.
43. Oshumi, Katagiri C, Kishimoto T (1993): Chromosome condensation in Xenopus mitotic extracts without histone H1. Science 262:2033-2035.
44. Paulson JR, Laemmli UK (1977): The structure of histone-depleted metaphase chromosomes. Cell 12:817-828.
45. Pienta KJ, Getzenberg RH, Coffey DS (1991): Cell structure and DNA organization. Crit Rev Eukary Gene Express 1:355-385.
46. Pickett-Heaps J, Spurck T, Tippit D (1984): Chromosome motion and the spindle matrix. J Cell Biol 99:137s-143s.
47. Ruiz-Carrillo A, Puigdomenech P, Eder G, Lurz R (1980): Stability and reversibility of higher ordered structure of interphase chromatin: Continuity of deoxyribonucleic acid is not required for maintenance of folded structure. Biochemestry 19:2544-2554.
48. Schmit AC, Lambert AM (1987): Characterization and dynamics of cytoplasmic F-actin in higher plant endosperm cells during interphase, mitosis, and cytokinesis. J Cell Biol 105:2157-2166.
49. Sluder G, Miller F, Thompson E, Wolf D (1994): Feedback control of the metaphase - anaphase transition in Sea Urchin Zygotes: Role of maloriented chromosomes. J Cell Biol 126:189-198.
50. Sen D, Gilbert W (1988): Formation of the parallel four-stranded complexes by guaninine-rich motifs in DNA and its implications for meiosis. Nature 334:364-366.
51. Sims J, Karp S, Ingber DE (1992): Altering the cellular mechanical force balance results in integrated changes in cell, cytoskeletal, and nuclear shape. J Cell Sci 103:1215-1222.
52. Smith SB, Finzi L, Bustamante C (1992): Direct mechanical measurements of the elasticity of single DNA molecules by using magnetic beads. Science 258:1122-1126.
53. Stemple DL, Sweet SC, Welsh MJ, MacIntosh JR (1988): Dynamics of a fluorescent calmodulin analog in the mammalian mitotic spindle at metaphase. Cell Motil Cytoskel 9:231-242.
54. Stief DM, Winter WH, Stratling AE, Sippel W (1989): Overstretching B-DNA: the elastic response of individual double-stranded and single-stranded DNA molecules. Nature 341:343-345.
55. Takayama S (1975): Interchromosomal connectives in squash preparations of L-cells. Exp Cell Res 91:409-412.
56. Thoma F, Koller T, Klug A (1979): Involvement of H1 in the organization of the nucleosome, and the salt-dependent substructures of chromatin. J Cell Biol 83:408-427.
57. Volgelstein B, Pardoll DM, Coffey DS (1980): A fixed site of DNA replication in eukaryotic cells. Cell 19:527-536.
58. Woodcock CLF, Frado LLY, Rattner JB (1984): The higher-order structure of chromatin: Evidence for a helical ribbon arrangement. J Cell Biol 99:42-52.
59. Yin B, Forer A (1996): Coordinated movements between autosomal half-bivalents in crane-fly spermatocytes: evidence that 'stop' signals are sent between partner half-bivalents. J Cell Sci 109:155-163.
60. Williamson JR, Raghuraman MK, Cech TR (1989): Monovalent cation-induced structure of telomeric DNA; The G-Quartet Model. Cell 59:871-880.