DNA: An extensible molecule

Science

Volumn 271, Issue 5250, 1996, Pages 792-794

  Cluzel, Philippe ^a   Lebrun, Anne ^b   Heller, Christoph ^a,e   Lavery, Richard ^b   Viovy, Jean Louis ^a   Chatenay, Didier ^c,f   Caron, François ^d  

^a INSTITUT CURIE   (France)

^b INST DE BIOLOGIE PHYSICO CHIMIQUE   (France)

^c UNIVERSITY OF LIÈGE   (Belgium)

^d ECOLE NORMALE SUPÉRIEURE   (France)

^e MAX PLANCK INSTITUTE FOR MOLECULAR GENETICS   (Germany)

^f ROCKEFELLER UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DOUBLE STRANDED DNA;

ARTICLE; DNA STRUCTURE; FORCE; MOLECULAR MODEL; PRIORITY JOURNAL;

EID: 0030064676     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.271.5250.792     Document Type: Article

References (28)

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14. Because this simple model does not take into account the entropic elasticity, it becomes rapidly inaccurate for extensions outside the plateau region. For a complete description, a theory taking into account the degrees of freedom related to the exchange between states, as well as those related to entropic elasticity (5), will be required Additional elasticity may also arise from distortions of bond angles (15) or from a dependence of transition energies on base pair content and sequence.
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22. EMBL3 λ DNA (16) was labeled at the nght end for attachment to a polystyrene microbead (Polyscience; 2.8 μm) covered with antibody to digoxigenin (anti-DIG) by ligation of a modified 12-mer oligonucleotide, complementary to the 5′ protruding right end of λ DNA The modification consists of labeling the oligonucleotide with terminal transferase in the presence of DIG 2′3′-dideoxyuridine-5′-triphosphate (Boehringer). At the left end, DNA was multilabeled (about 150 ligands) on both strands with biotin by the ligation, by means of an adapter, of a 700-base pair fragment labeled with biotin by the polymerase chain reaction.
23. The exact value of the initial extension, which is related to the position of the bead with respect to the anchonng of DNA on the fiber, is known only to within 3 μm, therefore, internal reference wfth a 1-μm accuracy was obtained by fitting the steep rise of the entropic force at the vicinity of the full contour length extension (4, 5) This fit yields a persistence length smaller than that obtained by others (5) by a factor of 3 to 4 This decrease is qualitatively consistent with our use of a higher salt concentration, but a quantitative study of salt effects woulc require a higher accuracy in the tow-force domain and is beyond the scope of our work here.
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28. The authors acknowledge support from the Chemistry Department of the CNRS, the Ultimatech program the DRET/Ministère des Armées, the European Eco nomic Community Biomed Program, and the Frenct Supercomputing Centre IDRIS. We thank J. Malthête and J. Davidovits for helpful discussions concerning the chemistry of these experiments, A Laigle for help ful preparatory experiments, and F. Breton for techni cal assistance.