Induced reporter gene activity, enhanced stress resistance, and competitive ability of a genetically modified Pseudomonas fluorescens strain released into a field plot planted with wheat

Applied and Environmental Microbiology

Volumn 63, Issue 5, 1997, Pages 1965-1973

  Van Overbeek, Leonard S ^a   Van Veen, Johannes A ^a   Van Elsas, Jan D ^a  

^a WAGENINGEN UNIVERSITY   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; BIODEGRADATION; CROP; GENE ACTIVITY; NONHUMAN; PSEUDOMONAS FLUORESCENS; REPORTER GENE; WHEAT;

PSEUDOMONAS; PSEUDOMONAS FLUORESCENS; TRITICUM AESTIVUM;

EID: 0030986085     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/aem.63.5.1965-1973.1997     Document Type: Article

References (53)

1. Bangera, G., and L. S. Thomashow. 1996. Characterization of a genomic locus required for synthesis of the antibiotic 2,4-diacetylphloroglucinol by the biological control agent Pseudomonas fluorescens Q2-87. Mol. Plant-Microbe Interact. 9:83-90.
2. Bashan, Y. 1991. Changes in membrane potential of intact soybean elongation zone cells induced by Azospirillum brasilense. Can. J. Microbiol. 37:958-963.
3. Bashan, Y., and H. Levanony. 1991. Alterations in membrane potential and in proton efflux in plant roots induced in Azospirillum brasilense. Plant Soil 137:99-103.
4. Beattie, G. A., and S. E. Lindow. 1994. Comparison of the behaviour of epiphytic fitness mutants of Pseudomonas syringae under controlled and field conditions. Appl. Environ. Microbiol. 60:3799-3808.
5. Beck, S. M., and C. M. Gilmour. 1983. Role of wheat root exudates in associative nitrogen fixation. Soil Biol. Biochem. 15:33-38.
6. Bosworth, A. H., M. K. Williams, K. A. Albrecht, R. Kwiatkowski, J. Beynon, T. R. Hankinson, C. W. Ronson, F. Cannon, T. J. Wacek, and E. W. Triplett. 1994. Alfalfa yield response to inoculation with recombinant strains of Rhizobium meliloti with an extra copy of dctABD and/or modified nifA expression. Appl. Environ. Microbiol. 60:3815-3832.
7. Brunel, B., J. C. Cleyet-Marel, P. Normand, and R. Bardin. 1988. Stability of Bradyrhizobium japonicum inoculants after introduction into soil. Appl. Environ. Microbiol. 54:2636-2642.
8. Christensen, H., L. R. Bakken, and R. A. Olsen. 1993. Soil bacterial DNA and biovolume profiles measured by flow-cytometry. FEMS Microbiol. Ecol. 102:129-140.
9. Colwell, R. R., P. R. Brayton, D. J. Grimes, D. B. Roszak, S. A. Huq, and L. M. Palmer. 1985. Viable but non-culturable Vibrio cholerae and related pathogens in the environment: implications for release of genetically engineered microorganisms. Bio/Technology 3:817-820.
10. Cook, R. J., L. S. Thomashow, D. M. Weller, D. Fujimoto, M. Mazzola, G. Bangera, and D. Kim. 1995. Molecular mechanisms of defence by rhizobacteria against root disease. Proc. Natl. Acad. Sci. USA 92:4197-4201.
11. De Leij, F. A. A. M., E. J. Sutton, J. M. Whipps, J. S. Fenlon, and J. M. Lynch. 1995. Impact of field release of genetically modified Pseudomonas fluorescens on indigenous microbial populations of wheat. Appl. Environ. Microbiol. 61:3443-3453.
12. Drahos, D. J., G. F. Barry, B. C. Hemming, E. J. Brandt, H. D. Skipper, E. L. Kline, D. A. Kluepfel, and D. T. Gooden. 1988. Pre-release testing procedures: US field test of a lacZY-engineered soil bacterium, p. 181-191. In M. Sussman, C. H. Collins, F. A. Skinner, and D. E. Stewart-Tull (ed.), the release of genetically-engineered micro-organisms, Academic Press Ltd., London, United Kingdom.
13. Evguenieva-Hackenberg, E., S. Selenska-Pobell, and W. Klingmüller. 1994. Persistence and stability of genetically manipulated derivatives of Enterobacter agglomerans in soil microcosms. FEMS Microb. Ecol. 15:179-192.
14. Fredrickson, J. K., S. A. Bentjen, H. Bolton, Jr., S. W. Li, and P. van Voris. 1989. Fate of Tn5 mutants of growth-inhibiting Pseudomonas sp. in intact soil-core microcosms. Can. J. Microbiol. 35:867-873.
15. Givskov, M., L. Eberl, S. Møller, L. K. Poulsen, and S. Molin. 1994. Responses to nutrient starvation in Pseudomonas putida KT2442: analysis of general cross protection, cell shape, and macromolecular content. J. Bacteriol. 176:7-14.
16. Gregory, P. J., and B. J. Atwell. 1991. The fate of carbon in pulse-labelled crops of barley and wheat. Plant Soil 136:205-213.
17. Heijnen, C. E., and J. C. Y. Marinissen. 1995. Survival of bacteria introduced into soil by means of transport by Lumbricus rubellus. Biol. Fertil. Soils 20: 63-69.
18. Heijnen, C. E., S. Page, and J. D. van Elsas. 1995. Metabolic activity of Flavobacterium strain P25 during starvation and after introduction into soil and the rhizospherc of wheat. FEMS Microbiol. Ecol. 18:129-138.
19. Hekman, W. E., C. E. Heijnen, S. L. G. E. Burgers, J. A. van Veen, and J. D. van Elsas. 1995. Transport of bacterial inoculants through intact cores of two different soils as affected by water percolation and the presence of wheat plants. FEMS Microbiol. Ecol. 16:143-158.
20. Hekman, W. E., C. E. Heijnen, J. T. Trevors, and J. D. van Elsas. 1994. Water flow induced transport of Pseudomonas fluorescens cells through soil columns as affected by inoculant treatment. FEMS Microbiol. Ecol. 13:313-324.
21. Jenkins, D. E., S. A. Chaisson, and A. Matin. 1990. Starvation-induced cross protection against osmotic challenge in Escherichia coli. J. Bacteriol. 172: 2779-2781.
22. 2 challenge in Escherichia coli. J. Bacteriol. 170:3910-3914.
23. Keith, H., J. M. Oades, and J. K. Martin. 1986. Input of carbon to soil from wheat plants. Soil Biol. Biochem. 18:445-149.
24. Liljeroth, E., E. Bååth, I. Mathiasson, and T. Lundhorg. 1990. Root exudation and rhizoplane bacterial abundance of barley (Hordeum vulgare L.) in relation to nitrogen fertilization and root growth. Plant Soil 127:81-89.
25. Liljeroth, E., S. L. G. E. Burgers, and J. A. van Veen. 1991. Changes in bacterial populations along roots of wheat (Triticum aestivum L.) seedlings. Biol. Fertil. Soils 10:276-280.
26. Lynch, J. M., and J. M. Whipps. 1990. Substrate flow in the rhizosphere. Plant Soil 129:1-10.
27. Martin, J. K. 1977. Effect of soil moisture on the release of organic carbon from wheat roots. Soil Biol. Biochem. 9:303-304.
28. Miller, J. H. 1974. Experiments in molecular genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
29. Moyer, C. L., and R. Y. Morita. 1989. Effect of growth rate and starvation survival on cellular DNA, RNA and protein of a psychrophilic marine bacterium. Appl. Environ. Microbiol. 55:2710-2716.
30. Natsch, A., C. Keel, J. Troxler, M. Zala, N. von Albertini, and G. Défago. 1996. Importance of preferential flow and soil management in vertical transport of a biocontrol strain of Pseudomonas fluorescens in structured field soil. Appl. Environ. Microbiol. 62:33-40.
31. Nesme, X., C. Picard, and P. Simonet. 1995. Specific DNA sequences for detection of soil bacteria, p. 111-139. In J. T. Trevors and J. D. van Elsas (ed.), Nucleic acids in the environment. Springer-Verlag KG, Berlin, Germany.
32. Postma, J., J. D. van Elsas, J. M. Govaert, and J. A. van Veen. 1988. The dynamics of Rhizobium leguminosarum biovar trifolii introduced into soil as determined by immunofluorescence and selective plating techniques. FEMS Microbiol. Ecol. 53:251-260.
33. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
34. Simon, R., J. Quandt, and W. Klipp. 1989. New derivatives of transposon Tn5 suitable for mobilization of replicons, generation of operon fusions and induction of genes in Gram-negative bacteria. Gene 80:161-169.
35. Smalla, K., N. Cresswell, L. C. Mendonca-Hagler, A. Wolters, and J. D. van Elsas. 1993. Rapid DNA extraction protocol from soil for polymerase chain reaction-mediated amplification. J. Appl. Bacteriol. 74:78-85.
36. Smalla, K., and J. D. van Elsas. 1995. Application of the PCR for detection of antibiotic resistance genes in environmental samples, p. 241-256. In J. T. Trevors and J. D. van Elsas (ed.), Nucleic acids in the environment. Springer-Verlag KG, Berlin, Germany.
37. Smit, E., J. D. van Elsas, J. A. van Veen, and W. M. de Vos. 1991. Detection of plasmid transfer from Pseudomonas fluorescens to indigenous bacteria in soil by using bacteriophage φR2f for counterselection. Appl. Environ. Microbiol. 57:3482-3488.
38. Tang, W., J. J. Pasternak, and B. R. Glick. 1995. Persistence in soil of the plant growth promoting rhizobacterium Pseudomonas putida GR12-2 and genetically manipulated derived strains. Can. J. Microbiol. 41:445-451.
39. Thomashow, L. S., and D. M. Weller. 1988. Role of a phenazine antibiotic from Pseudomonas fluorescens in biological control of Gaeumannomyces graminis var. tritici. J. Bacteriol. 170:3499-3508.
40. Trevors, J. T., J. D. van Elsas, M.-E. Starodub, and L. S. van Overbeek. 1989. Survival of and plasmid stability in Pseudomonas and Klebsiella spp. introduced into agricultural drainage water. Can. J. Microbiol. 35:675-680.
41. Trevors, J. T., J. D. van Elsas, L. S. van Overbeek, and M.-E. Starodub. 1990. Transport of a genetically engineered Pseudomonas fluorescens strain through a soil microcosm. Appl. Environ. Microbiol. 56:401-408.
42. Vančura, V. 1964. Root exudates of plants. I. Analysis of root exudates of barley and wheat in their initial phases of growth. Plant Soil 21:231-248.
43. van Elsas, J. D. 1992. Environmental pressure imposed on GEMMOs in soil, p. 1-14. In D. E. S. Steward-Tull and M. Sussman (ed.), The release of genetically modified microorganisms. Plenum Press, New York, N.Y.
44. van Elsas, J. D., A. F. Dijkstra, J. M. Govaert, and J. A. van Veen. 1986. Survival of Pseudomonas fluorescens and Bacillus subtilis introduced into two soils of different texture in field microplots. FEMS Microbiol. Ecol. 38:151-160.
45. van Elsas, J.D., J.T. Trevors, and L. S. van Overbeek. 1991. Influence of soil properties on the vertical movement of genetically-marked Pseudomonas fluorescens through large soil microcosms. Biol. Fertil. Soils 10:249-255.
46. van Elsas, J. D., and L. S. van Overbeek. 1993. Bacterial responses to soil stimuli, p. 55-79. In S. Kjelleberg (ed.), Starvation in bacteria. Plenum Press, New York, N.Y.
47. van Elsas, J. D., L. S. van Overbeek, A. M. Feldman, A. M. Dullemans, and O. de Leeuw. 1991. Survival of a genetically engineered Pseudomonas fluorescens in soil in competition with the parent strain. FEMS Microbiol. Ecol. 85:53-64.
48. van Elsas, J. D., L. S. van Overbeek, and R. Fouchier. 1991. A specific marker, pat, for studying the fate of introduced bacteria and their DNA in soil using a combination of detection techniques. Plant Soil 138:49-60.
49. van Overbeek, L. S. Unpublished results.
50. van Overbeek, L. S., L. Eberl, M. Givskov, S. Molin, and J. D. van Elsas. 1995. Survival of and induced stress resistance in Pseudomonas fluorescens cells residing in soil. Appl. Environ. Microbiol. 61:4202-1208.
51. van Overheek, L. S. and J. D. van Elsas. 1995. Root exudate-induced promoter activity in Pseudomonas fluorescens mutants in the wheat rhizosphere. Appl. Environ. Microbiol. 61:890-898.
52. van Overbeek, L. S., J. D. van Elsas, J. T. Trevors, and M.-E. Starodub. 1990. Long-term survival of and plasmid stability in Pseudomonas fluorescens and Klebsiella species and appearance of nonculturable cells in agricultural drainage water. Microb. Ecol. 19:239-249.
53. van Poucke, S. O., and H. J. Nelis. 1995. Development of a sensitive chemiluminometric assay for the detection of β-galactosidase in permeabilized coliform bacteria and comparison with fluorometry and colorimetry. Appl. Environ. Microbiol. 61:4505-4509.