Characterization of a genomic locus required for synthesis of the antibiotic 2,4-diacetylphloroglucinol by the biological control agent Pseudomonas fluorescens Q2-87

Molecular Plant-Microbe Interactions

Volumn 9, Issue 2, 1996, Pages 83-90

  Bangera, M Gita ^a   Thomashow, Linda S ^a,b  

^a WASHINGTON STATE UNIVERSITY   (United States)

^b AGRICULTURAL RESEARCH SERVICE   (United States)

Author keywords

Biocontrol; Gaeumannomyces graminis var. tritici

Indexed keywords

2,4 DIACETYLPHLOROGLUCINOL; 2,4-DIACETYLPHLOROGLUCINOL; ANTIINFECTIVE AGENT; DRUG DERIVATIVE; PHLOROGLUCINOL;

ARTICLE; BACTERIAL GENE; BACTERIUM CONJUGATION; BIOLOGICAL PEST CONTROL; BIOSYNTHESIS; COMPARATIVE STUDY; ESCHERICHIA COLI; GENETIC TRANSCRIPTION; GENETICS; GENOTYPE; METABOLISM; PHENOTYPE; PLASMID; PSEUDOMONAS FLUORESCENS; TRANSPOSON;

ANTI-BACTERIAL AGENTS; CONJUGATION, GENETIC; DNA TRANSPOSABLE ELEMENTS; ESCHERICHIA COLI; GENES, BACTERIAL; GENOTYPE; PEST CONTROL, BIOLOGICAL; PHENOTYPE; PHLOROGLUCINOL; PLASMIDS; PSEUDOMONAS FLUORESCENS; TRANSCRIPTION, GENETIC;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; PSEUDOMONAS; PSEUDOMONAS FLUORESCENS;

EID: 0030111230     PISSN: 08940282     EISSN: None     Source Type: Journal    
DOI: 10.1094/mpmi-9-0083     Document Type: Article

References (51)

1. Allan, G. G. 1971. Page 532 in: Lignins: Occurrence, Formation, Structure and Reactions. K. V. Sarkanen and C. H. Ludwig, eds. Wiley-Interscience; John Wiley & Sons, Inc., New York.
2. Birnboim, H. C., and Doly, J. 1979. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 7:1513-1523.
3. Bowden, K., Broadbent, J. L., and Ross, W. J. 1965. Some simple anthelmintics. Br. J. Pharmacol. 24:717-724.
4. Broadbent, D., Mabelis, R. P., and Spencer, H. 1976. C-Acetylphloroglucinols from Pseudomonas fluorescens. Phytochemistry 15:1785.
5. Corbell, N. A., Kraus, J., and Loper, J. E. 1995. A global regulator of secondary metabolite production in Pseudomonas fluorescens Pf-5. J. Bacteriol. 177:6230-6236.
6. Défago, G., Berling, C. H., Burger, U., Haas, D., Kahr, G., Keel, C., Voisard, C., Wirthner, P., and Wüthrich, B. 1990. Suppression of black root rot of tobacco and other root diseases by strains of Pseudomonas fluorescens: Potential applications and mechanisms. Pages 93-108 in: Biological Control of Soil-borne Plant Pathogens. D. Hornby, ed. C.A.B. International, Wallingford, U.K.
7. Ditta, G., Stanfield, S., Corbin, D., and Helsinki, D. R. 1980. Broad host range DNA cloning system for gram-negative bacteria: Construction of a gene bank of Rhizobium meliloti. Proc. Natl. Acad. Sci. USA 77: 7347-7351.
8. Donadio, S., Staverm, M. J., McAlpine, J. B., Swanson, S. J., and Katz, L. 1991. Modular organization of genes required for complex polyketide biosynthesis. Science 252:675-679.
9. Fenton, A. M., Crowley, J. J., Shanahan, P., and O'Gara, F. 1993. Cloning and heterologous exression of genes involved in 2,4-diacetyl-phloroglucinol biosynthesis in Psudomonas strains. Page 49 in: Int. Symp. Pseudomonas: Biotechnol. Mol. Biol., 4th. R. E. W. Hancock, ed.
10. Fenton, A. M., Stephens, P. M., Crowley, J., O'Callaghan, M., and O'Gara, F. 1992. Exploitation of gene(s) involved in 2,4-diacetyl-Phloroglucinol biosynthesis to confer a new biocontrol capability to a Pseudomonas strain. Appl. Environ. Microbiol. 58:3873-3878.
11. Fernádez-Moreno, M. A., Martinéz, E., Boto, L., Hopwood, D. A., and Malpartida, F. 1992. Nucleotide sequence and deduced functions of a set of cotranscribed genes of Streptomyces coelicolor A3(2) including the polyketide synthase for the antibiotic actinorhodin. J. Biol. Chem. 267:19278-19290.
12. Gaffney, T. D., Lam, S. T., Ligon, J., Gates, K., Frazalle, A., Di Maio, J., Hill, S., Goodwin, S., Torkewitz, N., Allshouse, A. M., Kempf, H.-J., and Becker, J. O. 1994. Global regulation of expression of antifungal factors by a Pseudomonas fluorescens biological control strain. Mol. Plant-Microbe Interact. 7:455-463.
13. Garagulya, A. D., Kiprianova, E. A., and Boiko, O. I. 1974. Antibiotic effect of bacteria from the genus Pseudomonas on phytopathogenic fungi. Mikrobiol. Zhumal (Kiev) 36:197-202.
14. Hamdan, H., Weller, D. M., and Thomashow, L. S. 1991. Relative importance of fluorescent siderophores and other factors in biological control of Gaeumannomyces graminis var. tritici by Pseudomonas fluorescens 2-79 and M4-80R. Appl. Environ. Microbiol. 57:3270-3277.
15. Harrison, L. A., Letendre, L., Kovacevich, P., Pierson, E. A., and Weller, D. M. 1993. Purification of an antibiotic effective against Gaeuman-nomyces graminis var. tritici produced by a biocontrol agent, Pseudomonas aureofaciens. Soil Biol. Biochem. 25:215-221.
16. Hopwood, D. A., and Sherman, D. H. 1990. Molecular genetics of polyketides and its comparison to fatty acid bisynthesis. Annu. Rev. Genet. 24:37-66.
17. Kataryan, B. T., and Torgashova, G. G. 1976. Spectrum of herbicidal activity of 2,4-diacetylphloroglucinol. Dok. Akad. Nauk. Arm. SSR 63: 109-112.
18. Keel, C., Schnider, U., Maurhofer, M., Voisard, C., Lavilee, J., Burger, U., Wirthner, P., Haas, D., and Défago, G. 1992. Supperssion of root diseases by Pseudomonas fluorescens CHA0: Importance of the bacterial secondary metabolite 2,4-diacetylphloroglucinol. Mol. Plant-Microbe Interact. 5:4-13.
19. Keel, C., Weller, D. M., Natsch, A., Défago, G., Cook, R. J., and Thomashow, L. S. Conservation of the biosythetic locus for 2,4-diacethylphloroglucinol among fluorescent Pseudomonas strains from diverse geographic locations. Appl. Environ. Microbiol. (In press.)
20. Keel, C., Wirthner, P. H., Oberhansli, T. H., Voisard, C., Burger, U., Haas, D., and Défago, G. 1990. Pseudomonads as antagonists of plant pathgens in the rhizophere: Role of the antibiotic 2,4-diacetyl-phloroglucinol in the suppression of black root rot of tobacco. Symbiosis 9:327-341.
21. Keen, H. T., Tanaju, S., Kobayashi, D., and Trollinger, D. 1988. Improved broad-host-range plasmids for DNA cloning in gram-negative bacteria. Gene 70:191-197.
22. King, E. O., Ward, M. K., and Raney, D. E. 1954. Two simple media for the demonstration of pyocyanin and fluorescein. J. Lab. Clin. Med. 44:301-307.
23. Kraus, J., and Loper, J. E. 1992. Lack of evidence for a role of antifungal metabolite production by Pseudomonas fluorescens Pf-5 in biological control of Pythium damping-off of cucumber. Phytopathology 82:264-271.
24. Kraus, J., and Loper, J. E. 1995. Characterization of a genomic region required for production of the antibiotic pyoluteorin by the biological control agent Pseudomonas fluortscens Pf-5. Appl. Environ. Microbiol. 61:849-854.
25. Kretschmer, P. J., and Cohen, S. N. 1977. Selected translocation of plasmid genes: Frequency and regional specificity of translocation of the Tn3 element. J. Bacteriol. 130:888-899.
26. Laville, J., Voisard, C., Keel, C., Maurhofer, M., Défago, G., and Haas, D. 1992. Global control in Pseudomonas fluorescens mediating antibiotic synthesis and suppression of black root rot of tobacco. Proc. Natl. Acad. Sci. USA 89:1562-1566.
27. Levy, E., Gough, F. J., Berlin, K. D., Guiana, P. W., and Smith, J. T. 1992. Inhibition of Septoria tritici and other phytopathogenic fungi and bacteria by Pseudomonas fluorescens and its antibiotics. Plant Pathol. 41:335-341.
28. Liyanage, H., Palmer, D. A., Ullrich, M., and Bender, C. L. 1995. Characterization and transcriptional analyses of the gene cluster for coronafacic acid, the polyketide component of the phytotoxin coronatine. Appl Environ. Microbiol. 61:3843-3848.
29. Marsh, J. L. Erfle, M., and Wykes, E. J. 1984 The pIC plasmid and phage vectors with versatile cloning sites for recombinant selection by insertional inactivation. Gene 32:481-485.
30. Martin, C. R. 1993. Structure, function, and regulation of chalcone synthase. Int. Rev Cytol. 147:233-283
31. Maurhofer, M., Keel, C., Haas, D., and Défago, G. 1995. Influence of plant species on disease suppression by Pseudomonas fluorescens strain CHA0 with enhanced antibiotic production. Plant Pathol. 44: 40-50.
32. Maurhofer, M., Keel, C., Schnider, U., Voisard, C., Haas, D., and Défago, G. 1992. Influence of enhanced antibiotic production in Pseudomonas fluorescens strain CHA0 on its disease suppressive capacity. Phytopathology 82:190-195.
33. Morrison, D. A. 1979. Transformation and preservation of competent bacterial cells by freezing. Methods Enzymol. 68:326-331.
34. Nowak-Thompson, B., Gould, S. J., Kraus, J., and Loper, J. E. 1994. Production of 2,4-diacetylphloroglucinol by the biocontrol agent Pseudomonas fluorescens Pf-5. Can. J. Microbiol. 40:1064-1066.
35. Pidoplichko, V. N., and Garagulya A. D. 1974. Effect of antagonistic bacteria on the development of wheat root rot. Mikrobiol. Zhurnal (Kiev) 36:599-602.
36. Pierson, E. A., and Weller, D. M. 1994. Use of mixtures of fluorescent Pseudomonads to suppress take-all and improve the growth of wheat. Phytopathology 84:940-947.
37. Pireson, L. S., III and Thomashow, L. S. 1992. Cloning and heterologous expression of the phenaine biosynthetic locus from Pseudomonas aureofaciens 30-84. Mol. Plant-Microbe Interact. 5:330-339.
38. Reddi, T. K. K., and Borovkov, A. V. 1970. Antibiotic properties of 2,4-diacetylphloroglucinol (2,4-diacetyl-1,3,5-trihydroxybenzene) produced by Pseudomonas fluorescens strain 26-O. Avtibiotiki (Moscow) 15:19-21.
39. Reddi, T. K. K., Khudyakov, Y. P., and Borovkov, A. V. 1969. Pseudomonas fluorescens strain 26-O, a producer of phytotoxic substances. Mikrobiologiya 38:909-913.
40. Sambrook, J., Fritsch, E. F., and Maniatis, T. A. 1989. Molecular Cloning: A Laboratory Manual. 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.
41. Schnider, U., Keel, C., Blumer, C., Troxler, J. Défago, G., and Haas, D. 1995. Amplification of the house-keeping sigma factor in Pseudomonas fluorescens CHAO enhances antibiotic production and improves biocontrol abilities. J. Bacteriol. 177:5387-5392.
42. Schröder, J., and Schröder, G. 1990. Stilbene and chalcone synthases: related enzymes with key functions in plant-specific pathways. Z. Naturforsch. 45c:1-8.
43. Shanahan, P., Glennon, J. D., Crowley, J. J., Donnelly, D. F. and O'Gara, F. 1993. Liquid Chromatographic assay of microbially derived phloroglucinol antibiotics for establishing the biosynthetic route to production, and the factors affecting their regulation. Anal. Chim. Acta 272:271-277.
44. Shanahan, P., O'Sullivan, D. J., Simpson, P., Glennon, J. D., and O'Gara, F. 1992. Isolation of 2,4-diacetylphloroglucinol from a fluorescent pseudomonad and investigation of physiological parameters influencing its production Appl. Environ. Microbiol 58:353-358.
45. Stachel S. E., An, G., Flores, C., and Nester, E. W. 1985. A Tn3-lacZ transposon for the random generation of β-galactosidase gene fusions: Application to the analysis of gene expression in Agrobacterium. EMBO J. 4:891-898.
46. Tada, M., Takakuwa, T., Nagai, M., and Yoshii, T. 1990. Antiviral and antimicrobial activity of 2,4-diacetylphloroglucinols, 2-acetylcyclohexane-1,3-dones and 2-carboxamidecylohexane-1,3-diones. Agric. Biol. Chem. 54:3061-3063.
47. Thomashow, L. S., and Weller, D. M. 1988. Role of a phenazine antibiotic from Pseudomonas fluorescens in biological control of Gaeumannomyces graminis var. tritici. J. Bacteriol. 170:3499-3508.
48. Ullrich, M., Gkuenzi, A. C., Mitchell, R. E. and Bender, C. L. 1994, Cloning and expression of genes required for coronamic acid (2-ethyl-1-aminocyclopropane 1-carboxylic acid), an intermediate in the biosynthesis of the phytotoxin coronatine. Appl. Environ. Microbiol. 60: 2890-2897.
49. Vincent, M. N., Harrison, L. A., Brackin, J. M. Kovacevich, P. A. Mukerji, P., Weller, D. M., and Pierson, E. A. 1991. Genetic analysis of the antifungal activity of a soilborne Pseudomonas aureofaciens strain. Appl. Environ. Microbiol. 57:2928-2934.
50. Weller, D. M., and Cook, R. J. 1983. Suppression of take-all wheat by seed treatments with fluorescent pseudomonads. Phytopathology 73: 463-469.
51. Willis, D. K., Rich, J. J., Kinscherf, T. G., and Kitten, T. 1994. Genetic regulation in plant pathogenic pseudomonads. Pages 167-193 in: Genetic Engineering, Vol. 15. J. T. Suslow, ed. Plenum Press, New York.