Generation of targeted nonpolar gene insertions and operon fusions in Pasteurella haemolytica and creation of a strain that produces and secretes inactive leukotoxin

Infection and Immunity

Volumn 65, Issue 7, 1997, Pages 2593-2598

  Fedorova, Natalie D ^a   Highlander, Sarah K ^a  

^a BAYLOR COLLEGE OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

LEUKOTOXIN;

ARTICLE; CHROMOSOMAL LOCALIZATION; GENE CLUSTER; GENE FUSION; GENE INSERTION; GENE TARGETING; GROWTH CURVE; MANNHEIMIA HAEMOLYTICA; NONHUMAN; OPERON; PLASMID; PRIORITY JOURNAL; PROMOTER REGION; STRAIN DIFFERENCE;

EID: 0030979509     PISSN: 00199567     EISSN: None     Source Type: Journal    
DOI: 10.1128/iai.65.7.2593-2598.1997     Document Type: Article

References (46)

1. Azud, A. K., J. G. Coote, and R. Parton. 1994. Construction of conjugative shuttle and suicide vectors for Pasteurella haemolytica and P. multocida. Gene 145:81-85.
2. Briggs, R. E., F. M. Tatum, T. A. Casey, and G. H. Frunk, 1994. Characterization of a restriction endonuclease. PhaI, from Pasteurella haemolytica serotype A1 and protection of heterologous DNA by a cloned PhaI methyltransferase gene. Appl. Environ. Microbiol. 60:2006-2011.
3. Chang, Y. F., D. P. Ma, H. Q. Bai, R. Young, D. K. Struck, S. J. Shin, and D. H. Lein. 1992. Characterizatioin plasmids with antimicrobial resistant genes in Pasteurella haemolytica A J. DNA Seq. Map. 3:89-97.
4. Chang, Y. F., D. ost, and D. K. Struck. 1987. Identification and characterization of the Pasteurella haemolytica leukotoxin. Infect. Immun. 55:2348-2354.
5. Clinkenbeard, K. D., D. A. Mosier, and A. W. Confer. 1989. Transmembrane pore size and role of cell swelling in cytotoxicity caused by Pasterella haemolytica leukotoxin. Infect. Immun. 57:420-425.
6. Confer, A. W., R. J. Panciera, K. D. Clinkenbeard, and D. A. Mosier. 1990. Molecular aspects of virulence of Pasteurella haemolytica. Can. J. Vet. Res. 54:S48-S52.
7. Conlon, J. A., P. E. Shewen, and R. Y. C. Lo. 1991. Efficacy of recombinant leukotoxin in protection against pneumonic challenge with live Pasteurella haemolytica A1. Infect. Immun. 59:587-591.
8. Craig, F. F., J. G. Coote, R. Parton, J. H. Freer, and J. L. Gilmour. 1989. A plasmid which can be transferred between Escherichia coli and Pasteuirella haemoytica by electroporation and conjugation. J. Gen. Microbiol. 135:2885-289.
9. Diker, K. S., M. Akan, and R. Haziroglu. 1994. Antimicrobial susceptibility of Pasteurella haemolytica and Pasteurella mutltocida isolated from pneumonic ovine lungs. Vet. Rec. 134:597-598.
10. Dixon, L. G., W. L. Albritton, and P. J. Willson. 1994. An analysis of the complete nucleotide sequence of the Haemophilus ducreyi broad-host-range plasmid pLS88. Plasmid 32:228-232.
11. Dower, W. J., J. F. Miller, and C. R. Ragsdale. 1988. High efficiency transformation of E. coli by high voltage electroporation. Nucleic Acids Res. 16:6127-6145.
12. Fedorova, N. D., and S. K. Highlander. 1997. Plasmids for heterologous expression in Pasteurella haemolytica. Gene 186:207-211.
13. Forestier, C., and R. A. Welch. 1990. Nonreciprocal complementation of the hlyC and lktC genes of the Escherichia coli haemolysin and Pasteurella haemolytica leukotoxin determinants. Infect. Immun. 58:828-832.
14. Krank, G. H. 1989. Pasteurellosis of cattle, p. 197-222. In C. Adlam and J. M. Rutters (ed.), Pasteurella and pusteurellosis. Academic Press. San Diego, Calif.
15. Frey, J., H. van den Bosch, R. Sugers, and J. Nicolet. 1992. Identification of a second hemolysin (HlyII) in Actinobacillus pleuropneumoniae serotype 1 and expression of the gene in Escherichia coli. Infect. Immun. 60:1671-1676.
16. Gentry, M. J., and S. Srikumaran. 1991. Neutralizing monoclonal antibodies to Pasteurella haemolytica leukotoxin affinity-purify the toxin from crude culture supernatants. Microb. Pathog. 10:410-417.
17. Gutterson, N. I., and D. E. Koshland. 1983. Replacement and amplification of bacterial genes with sequences altered in vitro. Proc. Natl. Acad. Sci. USA 80:4894-4998.
18. Highlander, S. K. Unpublished data.
19. Highlander, S. K., M. Chidambaram, M. J. Engler, and G. M. Weinstock. 1989. DNA sequence of the Pasteurella haemolytica leukotoxin gene cluster. DNA Cell Biol. 8:15-28.
20. Highlander, S. K., M. J. Engler, and G. M. Weinstock. 1990. Secretion and expression of the Pasxteurela haemolytica leukotoxin. J. Bacteriol. 172:2343-2350.
21. Highlander, S. K., and O. Garza. 1996. The restriction-modification system of Pasteurella haemolytica is a member of a new family of type I enzymes. Gone 178:89-96.
22. Highlander, S. K., and G. M. Weinstock. 1994. Static DNA bending and protein interactions within the Pasteurella haeamolytica leukotoxin promoter region: development of an activation model for leukotoxin transcriptional control. DNA Cell Biol. 13:171-181.
23. Highlander, S. K., E. A. Wickersham, O. Garza, and G. M. Weinstock. 1993. Expression of the Pasteurella haemolytica leukotoxin is inhibited by a locus that encodes an ATP-binding cassette homolog. Infect. Immun. 61:3942-3951.
24. Homehampa, P., R. A. Strugnell, and B. Adler. 1994. Construction and vaccine potential of an aroA mutant of Pasteurella haemolytica. Vet. Microbiol. 42:35-44.
25. Issurtel, J.-P., V. Koronakis, and C. Hughes. 1991. Activation of Escherichia coli prohaemolysin to the mature toxin by acyl carrier protein-dependent fatly acylation. Nature 351:759-761.
26. LO, R. Y, C., C. A. Strathdee, and P. E. Shewen. 1987. Nucleotide sequence of the leukotoxin genes of Pasteurella haemolylica A1. Infect. Immun. 55:1987-1996.
27. Lukomski, S., R. A. Hull, and S. I. Hull. 1996. Identification of the O antigen polymerase (rfc) gene in Escherichia coli O4 by insertional mutagenesis using a nonpolar chloramphenicol resistance cassette. J. Bacteriol. 178:240-247.
28. Murphy, G. L., and L. C. Whitworth. 1994. Construction of isogenic mutants of Pasteurella haemolylica by allelic replacement. Gene 148:101-105.
29. Murphy, G. L., L. C. Whitworth, K. D. Clinkenbeard, and P. A. Clinkenbeard. 1995. Hemolytic activity of the Pasteurella haemolytica leukotoxin. Infect. Immun. 63:3209-3212.
30. Neumann, J. R., C. A. Morency, and K. O. Russian. 1987. A novel rapid assay for chloramphenicol acetyltransferase gene expression. BioTechniques 5:444-447.
31. Petras, S. M., M. Chidambaram, E. F. Illyes, S. Froshauer, G. M. Weinstock, and C. P. Reese. 1995. Antigenic and virulence properties of Pasteurella haemolytica leukotoxin mutants. Infect. Immun. 63:1033-1039.
32. Ruvkun, G. B., and F. M. Ausubel. 1981. A general method for site-directed mutagenesis in prokaryotes. Nature 289:85-88.
33. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
34. Shaw, W. V. 1975. Chloramphenicol acetyltransferase from chloramphenicol-resistant bacteria. Methods Enzymol. 43:737-755.
35. Shewen, P. E., and B. N. Wilkie. 1982. Cytotoxin of Pasteurella haemolytica acting on bovine leukocytes. Infect. Immun. 35:91-94.
36. Shewen, P. E., and B. N. Wilkie. 1985. Evidence for the Pasteurella haemolytica cytotoxin as a product of actively growing bacteria. Am. J. Vet. Res. 46:1212-1214.
37. Stevens, P. K., and C. J. Czuprynski, 1996. Pasteurella haemolytica leukotoxin induces bovine leukocytes to undergo morphologic changes consistent with apoptosis in vitro. Infect. Immun. 64:2687-2694.
38. Strathdee, C. A., and R. Y. C. Lo. 1989. Regulation of expression of the Pasteurella haemolytica leukotoxin determinant. J. Bactcriol. 171:5955-5962.
39. Strathdee, C. A., and Y. C. Lo. 1987. Extensive homology between the leukotoxin of Pasteurella haemolytica A1 and the alpha-hemolysin of Escherichia coli. Infect. Immun. 55:3233-3236.
40. Tatum, F. M., R. E. Briggs, and M. Halling. 1994. Molecular gene cloning and nucleotide sequencing and construction of an aroA mutant of Pasteurella haemolytica serotype A1. Appl. Environ. Microbiol. 60:2011-2016.
41. Wagner, W., M. Kuhn, and W. Goebel. 1988. Active and inactive forms of hemolysin (HlyA) from Escherichia coli. Mol. Microbiol. 369:39-46.
42. Waurzyniak, B. J., K. D. Clinkenbeard, A. W. Confer, and S. Srikumaran. 1994. Enhancement of Pasteurella haemolytica leukotoxin activity by bovine serum albumin. Am. J. Vet. Res. 55:1267-1274.
43. Welch, R. A. 1991. Pore-forming cytolysins of Gram-negative bacteria. Mol. Microbiol. 5:521-528.
44. West, S. E. H., M. J. M. Romero, L. B. Regassa, N. A. Zielinski, and R. A. Welch. 1995. Construction of Actinobacillus pleuropneumoniae-Escherichia coli shuttle vectors: expression of antibiotic-resistance genes. Gene 160:81-86.
45. Wood, A. R., F. A. Lainson, F. Wright, G. D. Baird, and W. Donachie. 1995. A native plasmid of Pasteurella haemolytica serotypc A1: DNA sequence analysis and investigation of its potential as a vector. Res. Vet. Sci. 58:163-168.
46. Wright, K. L., R. A. Strugnell, and A. L. M. Hodgson. 1997. Characterization of a Pasteurella multocida plasmid and its use to express recombinant proteins in P. multocida. Plasmid 37:65-79.