Targeted disruption of the gene encoding the classical nitroreductase enzyme in Salmonella typhimurium Ames test strains TA1535 and TA1538

Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis

Volumn 375, Issue 1, 1997, Pages 9-17

  Yamada, Masami ^a   Espinosa Aguirre, Javier J ^b,c   Watanabe, Masahiko ^a,d   Matsui, Keiko ^a   Sofuni, Toshio ^a   Nohmi, Takehiko ^a  

^a NATIONAL INSTITUTE OF HEALTH SCIENCES   (Japan)

^b UNIVERSIDAD NACIONAL AUTÓNOMA DE MÉXICO   (Mexico)

^c INSTITUTO NACIONAL DE PEDIATRÍA   (Mexico)

^d NATIONAL CANCER CENTER RESEARCH INSTITUTE   (Japan)

Author keywords

Classical nitroreductase; Mutagenicity; nfnB; nfsB; Nitro compound; Salmonella typhimurium

Indexed keywords

NITRO DERIVATIVE; NITROREDUCTASE;

AMES TEST; ARTICLE; GENE DISRUPTION; MUTAGEN TESTING; MUTAGENICITY; NONHUMAN; PRIORITY JOURNAL; SALMONELLA TYPHIMURIUM;

BLOTTING, SOUTHERN; CHROMOSOME MAPPING; ESCHERICHIA COLI; IN SITU HYBRIDIZATION; MUTAGENICITY TESTS; MUTATION; NITRO COMPOUNDS; NITROREDUCTASES; SALMONELLA TYPHIMURIUM;

EID: 0030978166     PISSN: 00275107     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0027-5107(96)00243-6     Document Type: Article

References (38)

1. Bachmann, B. (1990) Linkage map of Escherichia coli K-12, edition 8, Microbiol. Rev., 54, 130-197.
2. Bryant, C., L. Hubbard and W.D. McElroy (1991) Cloning, nucleotide sequence and expression of the nitroreductase gene from Enterobacter cloacae, J. Biol. Chem., 266, 4126-4130.
3. Bryant, D.W., D.R. McCalla, M. Leeksma and P. Laneuville (1981) Type I nitroreductases of Escherichia coli, Can. J. Microbiol., 27, 81-86.
4. Einistö, P. (1991) Role of bacterial nitroreductase and O-acetyltransferase in urine mutagenicity assay of rats exposed to 2,4,6-trinitrotoluene, Mutation Res., 262, 167-169.
5. Espinosa-Aguirre, J.J., R.E. Reyes, J. Rubio, P. Ostrosky-Wegman and G. Martinez (1993) Mutagenic activity of urban air samples and its modulation by chili extracts, Mutation Res., 303, 55-61.
6. Hagiwara, Y., M. Watanabe, Y. Oda, T. Sofuni and T. Nohmi (1993) Specificity and sensitivity of Salmonella typhimurium YG1041 and YG1042 strains possessing elevated levels of both nitroreductase and acetyltransferase activity, Mutation Res., 291, 171-180.
7. Harada, N. and T. Omura (1980) Participation of cytochrome P-450 in the reduction of nitro compounds by rat liver microsomes, J. Biochem. (Tokyo), 87, 1539-1554.
8. Josephy, P.D., P. Gruz and T. Nohmi (1996) The evolution of bacterial mutagenicity assays, Mutation Res., in press.
9. Jurado, J., E. Alejandre-Duran and C. Pueyo (1994) Mutagenicity testing in Salmonella typhimurium strains possessing both the His reversion and Ara forward mutation systems and different levels of classical nitroreductase or O-acetyltransferase activities, Environ. Mol. Mutagen., 23, 286-293.
10. Kinouchi, T. and Y. Ohnishi (1983) Purification and characterization of 1-nitropyrene nitroreductases from Bacteroides fragilis, Appl. Environ. Micribiol., 46, 596-604.
11. Kohara, Y., K. Akiyama and K. Isono (1987) The physical map of the whole Escherichia coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library, Cell, 50, 495-508.
12. Maron, D.M. and B.N. Ames (1983) Revised methods for the Salmonella mutagenicity test, Mutation Res., 113, 173-215.
13. McCalla, D.R., C. Kaiser and M.H.L. Green (1978) Genetics of nitrofurazone resistance in Escherichia coli, J. Bacteriol., 133, 10-16.
14. McCoy, E.C., H.S. Rosenkranz and R. Mermelstein (1981) Evidence for the existence of a family of bacterial nitroreductases capable of activating nitrated polycyclics to mutagens, Environ. Mutagen., 3, 421-427.
15. McCoy et al. (1983) Mutation Res., 121, 17-23.
16. Michael, N.P., J.K. Brehm., G.M. Anlezark and N.P. Minton (1994) Physical characterization of the Escherichia coli B gene encoding nitroreductase and its over-expression in Escherichia coli K12, FEMS Microbiol. Lett., 124, 195-202.
17. Nohmi, T. and M. Matsui (Eds.) (1991) Mutagenicity tests using microorganisms: collection of data, LIC, Tokyo, pp. 197-198.
18. Pitts, J.N., Jr., K.A. van Cauwenberghe, D. Grosjean, J.P. Schmid, D.R. Fitz, W.L. Belser, G.B. Knudson Jr. and P.M. Hynds (1978) Atmospheric reactions of polycyclic aromatic hydrocarbons: facile formation of mutagenic nitro derivatives, Science, 202, 515-519.
19. Popkin, D.J., V.M. Davis and M.J. Prival (1989) Isolation and characterization of an isogenic set of Salmonella typhimurium strains analogous to the 'Ames' tester strains. Mutation Res., 224, 453-464.
20. Rosenkranz, H.S. and R. Mermelstein (1983) Mutagenicity and genotoxicity of nitroarenes. All nitro-containing chemicals were not created equal, Mutation Res., 114, 217-267.
21. Rosenkranz, H.S. and W.T. Speck (1975) Mutagenicity of metronidazole: activation by mammalian liver microsomes, Biochem. Biophys. Res. Commun., 66, 520-525.
22. Rosenkranz, H.S., E.C. McCoy, D.R. Sanders, M. Butler, D.K. Kiriazides and R. Mermelstein (1980) Nitropyrenes: isolation, identification, and reduction of mutagenic impurities in carbon black and toners, Science, 209, 1039-1043.
23. Sanderson, K.E. and J.R. Roth (1988) Linkage map of Salmonella typhimurium, edition VII, Microbiol. Rev., 52, 485-532.
24. Sastry, S.S. and R. Jayaraman (1984) Nitrofurantoin-resistant mutants of Escherichia coli: isolation and mapping, Mol. Gen. Genet., 196, 379-380.
25. Scheepers, P.T.J., J.L.G. Theuws and R.P. Bos (1991) Mutagenicity of urine from rats after 1-nitropyrene and 2-nitrofluorene administration using new sensitive Salmonella typhimurium strains YG1021 and YG1024, Mutation Res., 260, 393-399.
26. Southern, E.M. (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis, J. Mol. Biol., 98, 503-517.
27. Suzuki, J., K. Kuwayama and S. Suzuki (1992) Mutagenicity assay for nitroarenes of air pollutants held in leaves of woody plants, Mutation Res., 271, 89-96.
28. Wang, C.Y., B.C. Behrens, M. Ichikawa and G.T. Bryan (1974) Nitroreduction of 5-nitrofuran derivatives by rat liver xanthine oxidase and reduced nicotineamide adenine dinucleotide phosphate-cytochrome c reductase, Biochem. Pharmacol., 23, 3395-3404.
29. Wang, C.Y., M.-S. Lee, C.M. King and P.O. Warner (1980) Evidence for nitroaromatics as direct-acting mutagens of airborne particulates, Chemosphere, 9, 83-87.
30. Watanabe, M., M. Ishidate Jr. and T. Nohmi (1989) A sensitive method for the detection of mutagenic nitroarenes: construction of nitroreductase-overproducing derivatives of Salmonella typhimurium strains TA98 and TA100, Mutation Res., 216, 211-220.
31. Watanabe, M., M. Ishidate Jr. and T. Nohmi (1990) Nucleotide sequence of Salmonella typhimurium nitroreductase gene, Nucleic Acids Res., 18, 1059-1059.
32. Wei, C.-I., O.G. Raabe and L.S. Rosenblatt (1982) Microbial detection of mutagenic nitro-organic compounds in filtrates of coal fly-ash, Environ. Mutagen., 4, 249-258.
33. Wilson, K. (1987) Preparation of genomic DNA from bacteria, unit 2.4, in F.M. Ausubel et al. (Ed.), Current Protocols in Molecular Biology, John Wiley and Sons, New York.
34. Yamada, M., A. Hakura, T. Sofuni and T. Nohmi (1993) New method for gene disruption in Salmonella typhimurium: construction of an ada-deletion derivative of Salmonella typhimurium TA1535, J. Bacteriol., 175, 5539-5547.
35. Yergey, J.A., T.H. Risby and S.S. Lestz (1982) Chemical characterization of organic adsorbates on diesel particulate matter, Anal. Chem., 54, 354-357.
36. Yu, W.C., D.H. Fin, K.S. Chiu and K. Biemann (1984) Determination of nitrated polycyclic aromatic hydrocarbons in diesel particulates by gas chromatography with chemiluminescent detection, Anal. Chem., 56, 1158-1162.
37. Zenno, S., H. Koike, A.N. Kumar, R. Jayaraman, M. Tanokura and K. Saigo (1996a) Biochemical characterization of NfsA, the Escherichia coli major nitroreductase exhibiting a high amino acid sequence homology to Frp, a Vibrio harveyi flavin oxidoreductase, J. Bacteriol., 178, 4508-4514.
38. Zenno, S., H. Koike, M. Tanokura and K. Saigo (1996b) Gene cloning, purification and characterization of NfsB, a minor oxygen-insensitive nitroreductase from Escherichia coli, similar in biochemical properties to FRase I, the major flavin reductase in Vibrio fischeri, J. Biochem., in press.