Biochemical characterization of NfsA, the Escherichia coli major nitroreductase exhibiting a high amino acid sequence homology to Frp, a Vibrio harveyi flavin oxidoreductase

Journal of Bacteriology

Volumn 178, Issue 15, 1996, Pages 4508-4514

  Zenno, Shuhei ^a,b,c   Koike, Hideaki ^c   Kumar, Ajit N ^d   Jayaraman, Ramamirth ^d   Tanokura, Masaru ^c   Saigo, Kaoru ^a  

^a Graduate School of Science   (Japan)

^b CHISSO CORPORATION   (Japan)

^c UNIVERSITY OF TOKYO   (Japan)

^d MADURAI KAMARAJ UNIVERSITY   (India)

Author keywords

[No Author keywords available]

Indexed keywords

NITROREDUCTASE; OXIDOREDUCTASE; QUERCETIN;

AMINO ACID SEQUENCE; ARTICLE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME SPECIFICITY; ESCHERICHIA COLI; NONHUMAN; PRIORITY JOURNAL; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY; STRUCTURE ACTIVITY RELATION; VIBRIO HARVEYI;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; VIBRIO HARVEYI;

EID: 0029745672     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.178.15.4508-4514.1996     Document Type: Article

References (31)

1. Bachmann, B. J. 1990. Linkage map of Escherichia coli K-12, edition 8. Microbiol. Rev. 54:130-197.
2. Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-254.
3. Brew, K., J. H. Shaper, K. W. Olsen, I. P. Trayer, and R. L. Hill. 1975. Cross-linking of the components of lactose synthetase with dimethylpimelimidate. J. Biol. Chem. 250:1434-1444.
4. Bryant, C., and M. DeLuca. 1991. Purification and characterization of an oxygen-insensitive NAD(P)H nitroreductase from Enterobacter cloacae. J. Biol. Chem. 266:4119-4125.
5. Bryant, D. W., D. R. McCalla, M. Leeksma, and P. Laneuville. 1981. Type I nitroreductases of Escherichia coli. Can. J. Microbiol. 27:81-86.
6. Chou, P. Y., and G. D. Fasman. 1978. Empirical predictions of protein conformation. Annu. Rev. Biochem. 47:25-76.
7. Chung, C. T., S. L. Niemela, and R. H. Miller. 1989. One-step preparation of competent Escherichia coli: transformation and storage of bacterial cells in the same solution. Proc. Natl. Acad. Sci. USA 86:2172-2175.
8. Gavin, J. J., F. F. Ebetino, R. Frcedman, and W. E. Waterbury. 1966. The aerobic degradation of l-(5-nitrofurrylideneamino)-2-imidazolidinone (NF246) by Escherichia coli. Arch. Biochem. Biophys. 113:399-404.
9. Hastings, J. W., C. J. Potrikus, S. C. Gnpta, M. Kurfurst, and J. C. Makemson. 1985. Biochemistry and physiology of bioluminescent bacteria. Adv. Microb. Physiol. 26:235-291.
10. Inouye, S., S. Zenno, Y. Sakaki, and F. I. Tsuji. 1991. High-level expression and purification of apoaequorin. Protein Expr. Purif. 2:122-126.
11. Jablonski, E. and M. DeLuca. 1977. Purification and properties of the NADH and NADPH specific FMN oxidoreductases from Beneckea harveyi. Biochemistry 16:2932-2936.
12. Jablonski, E., and M. DeLuca. 1978. Studies of the control of luminescence in Beneckea harveyi: properties of the NADH and NADPH:FMN oxidoreductases. Biochemistry 17:672-678.
13. Rang, W.-K., T. Icho, S. Isono, M. Kitakawa, and K. Isono. 1989. Characterization of the gene rimK responsible for the addition of glutamic acid residues to the C-terminus of ribosomal protein S6 in Escherichia coli K12. Mol. Gen. Genet. 217:281-288.
14. Kohara, Y., K. Akiyama. and K. Isono. 1987. The physical map of whole. mli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50:495-508.
15. Kumar, A. N., and R. Jayaraman. 1991. Molecular cloning, characterization and expression of a nitrofuran reductase gene of Escherichia coli. 3. Biosci. 16:145-159.
16. Kyte, J., and R. F. Doolittle. 1982. A simple method for displaying the hydropathic character of a protein. J. Mol. Biol. 157:105-132.
17. Laemmli, U. K. 1970. Cleavage of structural proteins during the assemhly of the head of bacteriophage T4. Nature (London) 227:680-685.
18. Lei, B., M. Liu, S. Huang, and S.-C. Tu. 1994. Vibrio harveyi NADPH-flavin oxidoreductase: cloning, sequencing and overexpression of the gene and purification and characterization of the cloned enzyme. J. Bacteriol. 176: 3552-3558.
19. McCalla, D. R., C. Kaiser, and M. H. L. Green. 1978. Genetics of nitrofurazone resistance in Escherichia coli. 3. Bacteriol. 133:10-16.
20. Messing, J., and J. Vieira. 1982. A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments. Gene 19:269-276.
21. Ohnishi, K.. Y. Niimura, K. Yokoyama, M. Hidaka, H. Masaki, T. Uchimura, R Suzuki, T. Uozumi, M. Kozaki, K. Komagata, and T. Nishino. 1994 Purification and analysis of a flavoprotein functional as NADH oxidase from Amphibaciltus xylanus overexpressed in Escherichia coli. 3. Biol. Chem. 269: 31418-31423.
22. Peterson, F. J., R. P. Mason, J. Hovsepian, and J. L. Holtzruan. 1979. Oxygen-sensitive and -insensitive nitroreduction by Escherichia coli and rat hepatic microsomes. J. Biol. Chem. 254:4009-4014.
23. Rosenberg, M., and D. Court. 1979. Regulatory sequences involved in the promotion and termination of RNA transcription. Annu. Rev. Genet. 13: 319-353.
24. Sambrook, J., E. F. Fritsch, and T. Manialis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
25. Schwartz, R. M., and M. O. Dayhoff. 1978. Matrices for detecting distant relationships, p. 353-358. In M. O. Dayhoff (ed.), Atlas of protein sequence and structure, vol. 5, suppl. 3. National Biochemical Research Foundation, Washington, D.C
26. Shine, J., and L. Dalgarno. 1974. The 3'-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites. Proc. Natl. Acad. Sci. USA 71:1342-1346.
27. Uemori, T., Y. Ishinn, K. Fujita, K. Asada, and I. Kalo. 1993. Cloning of the DNA polymerase gene of Bacillus caldotenax and characterization of the gene product. J. Biochem. 113:401-410.
28. Zenno, S., H. Koike, M. Tanokura, and K. Saigo. 1996. Conversion of NfsB, a minor Escherichia coli nitrorcductase, to a flavin reductase similar in biochemical properties to FRase I, the major flavin reductase in Vibrio fischen, by a single amino acid substitution. J. Bacteriol. 178:4731-4733.
29. Zenno, S., H. Koike, M. Tanokura, and K. Saigo. Gene cloning, purification and characterization of NfsB, a minor oxygen-insensitive nitroreductase from Escherichia coli, similar in biochemical properties to FRase I, the major flavin reductase in Vibrio fischen. J. Biochem., in press.
30. Zenno, S., and K. Saigo. 1994. Identification of the genes encoding NAD(P)H-flavin oxidoreductases that are similar in sequence to Escherichia coli Fre in four species of luminous bacteria: Photorhabdus luminescent, Vibrio fischen. Vibrio harveyi, and Vibrio orientalis. J. Bacteriol. 176:3544-3551.
31. Zenno, S., K. Saigo, H. Kanoh, and S. Inouye. 1994. Identification of the gene encoding the major NAD(P)H-flavin oxidoreductase of the bioluminescent bacterium Vibrio fischeri ATCC 7744. J. Bacteriol. 176:3536-3543.