A novel use for a carbodiimide compound for the fixation of fluorescent and non-fluorescent calcium indicators in situ following physiological experiments

Cell Calcium

Volumn 21, Issue 3, 1997, Pages 175-183

  Tymianski, M ^a,b   Bernstein, G M ^a,b   Abdel Hamid, K M ^a,b   Sattler, R ^a,b   Velumian, A ^a   Carlen, P L ^a,b   Razavi, H ^b   Jones, O T ^a,b  

^a TORONTO WESTERN HOSPITAL   (Canada)

^b UNIVERSITY OF TORONTO   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

1 (3 DIMETHYLAMINOPROPYL) 3 ETHYLCARBODIIMIDE; CALCIUM CHELATING AGENT; CARBON 14; CYANAMIDE; ETHYLENE GLYCOL 1,2 BIS(2 AMINOPHENYL) ETHER N,N,N',N' TETRAACETIC ACID; FLUORESCENT DYE; LUCIFER YELLOW;

ANIMAL TISSUE; ARTICLE; AUTORADIOGRAPHY; BRAIN SLICE; CONTROLLED STUDY; ELECTROPHYSIOLOGY; ENZYME LINKED IMMUNOSORBENT ASSAY; FLUORESCENCE ANALYSIS; HIPPOCAMPUS; IMMUNOCYTOCHEMISTRY; KINETICS; NERVE CELL; NONHUMAN; PRIORITY JOURNAL; RAT;

ANIMALIA;

EID: 0030975427     PISSN: 01434160     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0143-4160(97)90042-7     Document Type: Article

References (40)

1. Tsien R.Y. New calcium indicators and buffers with high selectivity against magnesium and protons: design, synthesis, and properties of prototype structures. Biochemistry 1980; 19: 2396-2404.
2. Minta A., Kao J.P.Y., Tsien R.Y. Fluorescent indicators for cytosolic calcium based on rhodamine and fluorescein chromophores. J Biol Chem 1989; 264: 8171-8178.
3. Grynkiewicz G., Poenie M., Tsien R.Y. A new generation of calcium indicators with greatly improved fluorescence properties. J Biol Chem 1985; 260: 3440-3450.
4. Parsons T.D., Ellis-Davies G.C.R., Almers W. Millisecond studies of calcium-dependent exocytosis in pituitary melanotrophs: comparison of the photolabile calcium chelators nitrophenyl-EGTA and DM-nitrophen. Cell Calcium 1996; 19: 185-192.
5. Kaplan J.H. Photochemical manipulation of divalent cation levels. Annu Rev Physiol 1990; 52: 897-914.
6. 2+. Biophys J 1994; 66: 1879-1886.
7. Niggli E., Lederer W.J. Activation of Na-Ca exchange current by photolysis of 'caged calcium'. Biophys J 1993; 65: 882-891.
8. 2+-dependent slow afterhyperpolarization in hippocampal neurones: effects of internally applied anions. Pflügers Arch 1994; 426: 247-253.
9. 2+ chelators in hippocampal CAI neurons of rat brain slices. J Neurophysiol 1995; 74: 2225-2241.
10. Adler E.M., Augustine G.J., Duffy S.N., Charlton M.P. Alien intracellular calcium chelators attenuate neurotransmitter release at the squid giant synapse. J Neurosci 1991; 11: 1496-1507
11. Niesen C., Charlton M.P., Carlen R.L. Postsynaptic and presynaptic effects of the calcium chelator BAPTA on synaptic transmission in rat hippocampal dentate granule neurons. Brain Res 1991; 555: 319-325.
12. Robitaille R., Charlton M.P.. Presynaptic calcium signals and transmitter release are modulated by calcium-activated potassium channels. J Neurosci 1992; 12: 297-305.
13. Winslow J.L., Duffy S.N., Charlton M.P. Homosynaptic facilitation of transmitter release in crayfish is not affected by mobile calcium chelators: implications for the residual ionized calcium hypothesis from electrophysiological and computational analyses. J Neurophysiol 1994; 72: 1769-1793.
14. Spigelman I., Tymianski M., Wallace M.C., Carlen P.L., Velumian A.A. Modulation of hippocampal synaptic transmission by low concentrations of cell-permeant calcium chelators: effects of calcium affinity, structure, and binding kinetics. Neuroscience 1996; In press.
15. 2+ chelators reduce early excitotoxic and ischemic neuronal injury in vitro and in vivo. Neuron 1993; 11: 221-235.
16. i and glutamate neurotoxicity in vitro. J Neurophysiol 1994; 267: 1973-1992.
17. Scharfman B.E., Schwartzkroin P.A. Protection of dentate hilar cells from prolonged stimulation by intracellular calcium chelation. Science 1989; 246: 257-260.
18. Tsien R.Y. A non-disruptive technique for loading calcium buffers and indicators into cells. Nature 1981; 290: 527-528.
19. Madl J.E., Larson A.A., Beitz A.J. Monoclonal antibody specific for carbodiimide-fixed glutamate: immunohistochemical localization in the rat CNS. J Histochem Cytochem 1986; 34: 317-326.
20. Kendall P.A., Polak J.M., Pearse A.G.E. Carbodiimide fixation for immunohistochemistry: observations on the fixation of polypeptide hormones. Experientia 1971; 27: 1104-1106.
21. Yamamoto N., Yasuda K. Use of a water soluble carbodiimide as a fixing agent. Acta Histochem Cytochem 1977; 10: 14-37.
22. Polak J.M., Kendall P.A., Heath C.M., Pearse A.G.E. Carbodiimide fixation for electron microscopy and immunoelectron microscopy. Experientia 1972; 28: 368-370.
23. Nissinen M.J., Panula P. Histamine-storing cells in the oxyntic mucosa of the rat stomach: a transmission electron microscopic study employing fixation with carbodiimide. J Histochem Cytochem 1993; 41: 1405-1412.
24. Moffett J.R., Namboodiri M.A.A., Neale J.H. Enhanced carbodiimide fixation for immunohistochemistry: application to the comparative distributions of N-acetylaspartylglutamate and N-acetylaspartate immunoreactivities in rat brain. J Histochem Cytochem 1993; 41: 559-570.
25. Wang Z., Tymianski M., Jones O.T., Nedergaard M. Impact of cytoplasmic calcium buffering on the spatial and temporal characteristics of intercellular calcium signals in astrocytes. Neuron Submitted.
26. Abdel-Hamid K.M., Tymianski M. Protective and toxic potential of intracellular calcium buffering in organotypic hippocampal slice cultures exposed to anoxia/aglycemia or to excitotoxins. J Neurosci Submitted.
27. Staros J.V., Wright R.W., Swingle D.M. Enhancement by N-hydroxy sulfosuccinimide of water-soluble carbodiimide-mediated coupling reactions. Anal Biochem 1986; 156: 220-222.
28. Kemeny D.M. Practical guide to ELISA. Oxford: Pergamon, 1991.
29. Stoppini L., Buchs P.A., Muller D. A simple method for organotypic cultures of nervous tissue. J Neurosci Methods 1991; 37: 173-182.
30. Hertz E., Yu A.C.H., Hertz L., Juurlink B.H.J., Schousboe A. Preparation of primary cultures of mouse cortical neurons. In: Shahar A., de Vellis J., Vernadakis A., Haber B. (eds) A Dissection and Tissue Culture Manual of the Nervous System. New York: Alan R. Liss, 1989; 183-186.
31. McLean I.W., Nakane P.K. Periodate-lysine-paraformaldehyde fixative. A new fixation for immunoelectron microscopy. J Histochem Cytochem 1974; 22: 1077-1083.
32. Harlow E., Lane D. Antibodies: a Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory, 1988.
33. Panula P., Happola O., Airaksanen M.S., Auvinen S., Vikramaki A. A carbodiimide as a tissue fixative in histamine immunohistochemistry and its application in developmental neurobiology. J Histochem Cytochem 1988; 36: 259-269.
34. 2+ chelators in attenuation of synaptic transmission at hippocampal CA1 neurons. Neuroscience 1996; In press.
35. Munsch T., Deitmer J.W. Maintenance of fura-2 fluorescence in glial cells and neurones of the leech central nervous system. J Neurosci Methods 1995; 57: 195-204.
36. Khorana H.G. The chemistry of carbodiimides. Chem Rev 1953; 53: 145-166.
37. De Tar D.F., Silverstein R.J. Reactions of carbodiimides. II. The reactions of dicyclohexylcarbodiimide with carboxylic acids in the presence of amines and phenols. J Am Chem Soc 1966; 88: 1020-1023.
38. Gilles M.A., Hudson A.Q., Borders Jr C.L. Stability of water-soluble carbodiimides in aqueous solutions. Anal Biochem 1990; 184: 244-248.
39. Thelen P., Deuticke B. Chemo-mechanical leak formation in human erythrocytes upon exposure to a water-soluble carbodiimide followed by very mild shear stress. II. Chemical modifications involved. Biochim Biophys Acta 1988; 944: 297-307.
40. Kimberly D.K., Adikson A.A., Arthur K.M., Powers K.M., Shen D.D. Contribution of probenecid-sensitive anion transport processes at the brain capillary endothelium and choroid plexus to the efficient efflux of valproic acid from the central nervous system. J Pharmacol Exp Ther 1994; 268: 797-805.