Determining mineral content variations in bone using backscattered electron imaging

Bone

Volumn 20, Issue 5, 1997, Pages 485-490

  Bloebaum, R D ^a   Skedros, J G ^a   Vajda, E G ^a   Bachus, K N ^b   Constantz, B R ^c  

^a VETERANS AFFAIRS MEDICAL CENTER   (United States)

^b UNIVERSITY OF UTAH SCHOOL OF MEDICINE   (United States)

^c Norian Corporation   (United States)

Author keywords

Ash fraction; Backscattered electron imaging; Bone density; Calcified tissue; Microscopic; Mineral content

Indexed keywords

ANALYTICAL ERROR; ANIMAL TISSUE; ARTICLE; BIOMECHANICS; BONE DENSITY; BONE MINERAL; BONE REMODELING; BONE STRENGTH; CONTROLLED STUDY; CRYSTALLOGRAPHY; ELECTRON; EMBRYO; HUMAN; HUMAN TISSUE; IMAGING; INFRARED SPECTROSCOPY; MICRORADIOGRAPHY; NONHUMAN; X RAY DIFFRACTION; X RAY SPECTROMETRY;

ANIMALS; BONE AND BONES; BONE DENSITY; ELECTRONS; EVALUATION STUDIES; HUMANS; MICROSCOPY, ELECTRON, SCANNING; SCATTERING, RADIATION; SPECTROMETRY, X-RAY EMISSION; SPECTROSCOPY, FOURIER TRANSFORM INFRARED; X-RAY DIFFRACTION;

EID: 0030969087     PISSN: 87563282     EISSN: None     Source Type: Journal    
DOI: 10.1016/S8756-3282(97)00015-X     Document Type: Article

References (45)

1. Amprino, R. and Engstrom, A. Studies on x-ray absorption and diffraction of bone tissue. Acta Anat 15:1-22; 1952.
2. Bachus, K. N. and Bloebaum, R. D. Projection effect errors in biomaterials and bone research. Cells Mater 2:347-355; 1992.
3. Biltz, R. M. and Pellegrino, E. D. The chemical anatomy of bone. I. A comparative study of bone composition in sixteen vertebrates. J Bone Jt Surg [Am] 51-A:456-466; 1969.
4. Bloebaum, R. D., Bachus, K. N., and Boyce, T. M. Backscattered electron imaging: The role in calcified tissue and implant analysis. J Biomater Appl 5:56-85; 1990.
5. Boyce, T. M., Bloebaum, R. D., Bachus, K. N., and Skedros, J. G. Reproducible method for calibrating the backscattered electron signal for quantitative assessment of mineral content in bone. Scann Microsc 4:591-603; 1990.
6. Boyde, A., Elliott, J. C., and Jones, S. J. Stereology and histogram analysis of backscattered electron images: Age changes in bone. Bone 14:205-210; 1993.
7. Boyde, A. and Jones, S. J. Back-scattered electron imaging of skeletal tissues. Metab Bone Dis Rel Res 5:145-150; 1983.
8. Boyde, A. and Jones, S. J. Scanning electron microscopy of bone: Instrument, specimen, and issues. Microsc Res Technol 33:92-120; 1996.
9. Boyde, A., Jones, S. J., Aerssens, J., and Dequeker, J. Mineral density quantitation of the human cortical iliac crest by backscattered electron image analysis: Variations with age, sex, and degree of osteoarthritis. Bone 16:619-627; 1995.
10. Boyde, A., Maconnachie, E., Reid, S. A., Delling, G., and Mundy, G. R. Scanning electron microscopy in bone pathology: Review of methods, potential and applications. Scann Electron Microsc 4:1537-1554; 1986.
11. Cullity, B. D. Elements of X-Ray Diffraction. Reading, MA: Addison-Wesley; 1978; 99-106.
12. Currey, J. D. The mechanical consequences of variation in the mineral content of bone. J Biomech 2:1-11; 1969.
13. Currey, J. D. Mechanical properties of bone tissues with greatly differing functions. J Biomech 12:313-319; 1979.
14. Currey, J. D. The Mechanical Adaptations of Bones. Princeton, NJ: Princeton University Press; 1984; 88-97.
15. Currey, J. D. The effect of porosity and mineral content on the Young's modulus of elasticity of compact bone. J Biomech 21:131-139; 1988.
16. Driessens, F. and Verbeeck, R. The dynamics of bone mineral in some vertebrates. Z Naturforsch 41:468-471; 1986.
17. Emmanual, J., Hornbeck, C., and Bloebaum, R. D. A polymethyl methacrylate method for large specimens of mineralized bone with implants. Stain Technol 62:401-410; 1987.
18. Fratzl, P., Schreiber, S., Roschger, P., Lafage, M.-H., Rodan, G., and Klaushofer, K. Effects of sodium fluoride and alendronate on the bone mineral in minipigs: A small-angle X-ray scattering and backscattered electron imaging study. J Bone Miner Res 11:248-253: 1996.
19. Glimcher, M. J. The nature of the mineral component of bone and the mechanism of calcification, American Academy of Orthopedic Surgery, 62nd Annual Meeting (Instructional Course Lecture 105), 1995, 49-69.
20. Goldstein, J. I., Newbury, D. E., Echlin, P., Joy, D. C., Romig, A. D., Jr., Lyman, C. E., Fiori, C., and Lifshin, E. Scanning Electron Microscopy and X-ray Microanalysis. A Text for Biologists, Materials Scientists, and Geologists. New York: Plenum; 1992: 417-436.
21. Grynpas, M. D. and Holmyard, D. Changes in quality of bone mineral on aging and in disease. Scann Microsc 2:1045-1054: 1988.
22. Heinrich, K. F. J. Electron probe microanalysis by specimen current measurement. Proceedings of the Fourth International Congress on X-Ray Optics and Microanalysis. Paris: Hermann; 1966; 159-167.
23. Howell, P. G. T. and Boyde, A. Monte Carlo simulations of electron scattering in bone. Bone 15:285-291; 1994.
24. LeGeros, R. Z. Apatites in biological systems. Prog Cryst Growth Charact 4:1-45; 1981.
25. Lloyd, G. E. Atomic number and crystallographic contrast images with the SEM: A review of backscattered electron techniques. Mineralogical Magazine 51:3-19; 1987.
26. Lowenstam, H. and Weiner, S. On Biomineralization. New York: Oxford; 1989; 151.
27. Martin, R. B. and Burr, D. B. Structure, Function and Adaptation of Compact Bone. New York: Raven; 1989; 80-81.
28. Martin, R. B., Papamichos, T., and Dannucci, G. A. Linear calibration of radiographic mineral density using video-digitizing methods. Calcif Tissue Int 47:82-91; 1990.
29. McQueen, C. M., Smith, D. A., Monk, I. B., and Horton, P. W. A television scanning system for the measurement of the spatial variation of micro-density in bone sections. Calcif Tissue Res 11:124-132; 1973.
30. Pechak, D. G., Kujawa, M. J., and Caplan, A. I. Morphology of bone development and bone remodeling in embryonic chick limbs. Bone 7:459-472; 1986.
31. Reid, S. A. and Boyde, A. Changes in the mineral density distribution in human bone with age: Image analysis using backscattered electrons in the SEM. J Bone Miner Res 2:13-22; 1987.
32. Richelle, L. J. and Onkelinx, C. Recent advances in the physical biology of bone and other hard tissues. In: Comar, C. and Bronner, F., Eds. Mineral Metabolism: An Advanced Treatise. New York: Academic; 1969; 123-190.
33. Roschger, P., Plenk, H., Jr., Klaushofer, K., and Eschberger, J. A new scanning electron microscopy approach to the quantification of bone mineral distribution: Backscattered electron image grey-levels correlated to calcium Kα-line intensities. Scann Microsc 9:75-88; 1995.
34. Roufosse, A., Landis, W., Sabine, W., and Glimcher, M. Identification of brushite in newly deposited bone mineral from embryonic chicks. J Ultrastruc Res 68:235-255; 1979.
35. Rowland, R., Jowsey, J., and Marshall, J. Microscopic metabolism of calcium in bone. III. Microradiographic measurements of mineral density. Radiat Res 10:234-242; 1959.
36. Sanderson, C. Polymerization of mineralized bone specimens embedded in methylmethacrylate using ultraviolet irradiation. J Histotechnol 18:323-325; 1995.
37. Sanderson, C. and Kitabayashi, L. R. Parallel experience of two different laboratories with the initiator perkadox 16 for polymerization of methylmethacrylates. J Histotechnol 17:343-348: 1994.
38. Skedros, J. G., Bloebaum, R. D., Bachus, K. N., and Boyce, T. M. The meaning of gray levels in backscattered electron images of bone. J Biomed Mater Res 27:47-56; 1993.
39. Skedros, J. G., Bloebaum, R. D., Bachus, K. N., Boyce, T. M., and Constantz, B. Influence of mineral content and composition on graylevels in backscattered electron images of bone. J Biomed Mater Res 27:57-64; 1993.
40. Skedros, J. G., Mason, M. W., Nelson, M. C., and Bloebaum, R. D. Evidence of structural and material adaptation to specific strain features in cortical bone. Anat Rec 246:47-63; 1996.
41. Squillante, R. G. and Williams, J. L. Videodensitometry of osteons in females with femoral neck fractures. Calcif Tissue Int 52:273-277: 1993.
42. Sumner, D. R., Bryan, J. M., Urban, R. M., and Kuszak, J. R. Measuring the volume fraction of bone ingrowth: A comparison of three techniques. J Orthop Res 8:448-452; 1990.
43. Torontali, M., Koo, I., Holmyard, D. P., Tomlinson, G., Grynpas, M. D., and Tenenbraum, H. C. Backscattered electron image assessment of mineral density in bone formed in vitro. Cells Mater 4:125-134; 1994.
44. Vajda, E. G., Skedros, J. G., and Bloebaum, R. D. Consistency in calibrated backscattered electron images of calcified tissues and mineral analyzed in multiple imaging sessions. Scann Microsc 9:741-755; 1995.
45. Zou, L., Bloebaum, R. D., and Bachus, K. N. Reproducibility of techniques using Archimedes' principle in measuring cancellous bone volume. Med Eng Phys. In press.