Kinetics of transient radicals in Escherichia coli ribonucleotide reductase. Formation of a new tyrosyl radical in mutant protein R2

Journal of Biological Chemistry

Volumn 272, Issue 16, 1997, Pages 10414-10421

  Katterle, Bettina ^a   Sahlin, Margareta ^a   Schmidt, Peter P ^a   Pötsch, Stephan ^a   Logan, Derek T ^a   Gräslund, Astrid ^a   Sjöberg, Britt Marie ^a  

^a STOCKHOLM UNIVERSITY   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL ENZYME; MUTANT PROTEIN; RIBONUCLEOTIDE REDUCTASE;

AMINO ACID ANALYSIS; AMINO ACID SUBSTITUTION; ARTICLE; ENZYME ANALYSIS; ENZYME KINETICS; ENZYME STRUCTURE; ESCHERICHIA COLI; NONHUMAN; PRIORITY JOURNAL; STRUCTURE ANALYSIS;

CRYSTALLOGRAPHY, X-RAY; ELECTRON SPIN RESONANCE SPECTROSCOPY; ESCHERICHIA COLI; FREE RADICALS; KINETICS; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; OLIGODEOXYRIBONUCLEOTIDES; POINT MUTATION; PROTEIN CONFORMATION; RECOMBINANT PROTEINS; RIBONUCLEOTIDE REDUCTASES; SPECTROPHOTOMETRY; TYROSINE;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0030968307     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.272.16.10414     Document Type: Article

References (48)

1. Reichard, P. (1993) Science 260, 1773-1777
2. Sahlin, M., and Gräslund, A, (1996) Annu. Rev. Biophys. Biomol. Struct. 25, 259-286
3. Sjöberg, B.-M. (1994) Structure 2, 793-796
4. Nordlund, P., Sjöberg, B.-M., and Eklund, H. (1990) Nature 345, 593-598
5. Uhlin, U., and Eklund, H. (1994) Nature 370, 533-539
6. Un, S., Atta, M., Fontecave, M., and Rutherford, A. W. (1995) J. Am. Chem. Soc. 117, 10713-10719
7. Allard, P., Barra, A.-L., Andersson, K. K., Schmidt, P. P., Atta, M., and Gräslund, A. (1996) J. Am. Chem. Soc. 118, 895-896
8. Schmidt, P. P., Andersson, K. K., Barra, A.-L., Thelander, L., and Gräslund, A. (1996) J. Biol. Chem. 271, 23615-23618
9. Sahlin, M., Lassmann, G., Pötsch, S., Sjöberg, B.-M., and Gräslund, A. (1995) J. Biol. Chem. 270, 12361-12372
10. Nordlund, P., and Eklund, H. (1993) J. Mol. Biol. 232, 123-164
11. Atkin, C. L., Thelander, L., Reichard, P., and Lang, G. (1973) J. Biol. Chem. 248, 7464-7472
12. Åberg, A., Ormö, M., Nordlund, P., and Sjöberg, B.-M. (1993) Biochemistry 26, 5541-5548
13. Ochiai, E.-I., Mann, G., Gräslund, A., and Thelander, L. (1990) J. Biol. Chem. 265, 15758-15761
14. Bollinger, J. M., Edmondson, D. E., Huynh, B. H., Filley, J., Jr., Norton, J. R., and Stubbe, J. (1991) Science 253, 292-298
15. Sahlin, M., Lassmann, G., Pötsch, S., Slaby, A., Sjöberg, B.-M., and Gräslund, A. (1994) J. Biol. Chem. 269, 11699-11702
16. Bollinger, J. M., Jr., Stubbe, J., Huynh, B. H., and Edmondson, D. E. (1991) J. Am. Chem. Soc. 113, 6289-6291
17. Bollinger, J. M., Jr., Tong, W. H., Ravi, N., Huynh, B. H., Edmondson, D. E., and Stubbe, J. (1994) J. Am. Chem. Soc. 116, 8015-8023
18. Bollinger, J. M., Jr., Tong, W. H., Ravi, N., Huynh, B. H., Edmondson, D. E., and Stubbe, J. (1994) J. Am. Chem. Soc. 116, 8024-8032
19. Kraulis, P. J. (1991) J. Appl. Cryst. 24, 946-950
20. Lendzian, F., Sahlin, M., MacMillan, F., Bittl, R., Fiege, R., Pötsch, S., Sjöberg, B.-M., Gräslund, A., Lubitz, W., and Lassmann, G. (1996) J. Am. Chem. Soc. 118, 8111-8120
21. Sivaraja, M., Goodin, D. B., Smith, M., and Hoffman, B. (1989) Science 245, 738-740
22. Houseman, A. L. P., Doan, P. E., Goodin, D. B., and Hoffman, B. (1993) Biochemistry 32, 4430-4443
23. Lindström, B., Sjöqvist, B., and Anggard, E. J. (1974) J. Labelled Compd. 10, 187-194
24. Aschenbach, H., and König, F. (1972) Chem. Ber. 105, 784-793
25. Persson, B. O., Karlsson, M., Climent, I., Ling, J., Sanders-Loehr, J., Sahlin, M., and Sjöberg, B.-M. (1996) J. Biol. Inorg. Chem. 3, 247-256
26. Kunkel, T. A. (1985) Proc. Natl. Acad. Sci. U. S. A. 82, 488-492
27. Kunkel, T. A., Roberts, J. D., and Zakour, R. A. (1987) Methods Enzymol. 154, 367-382
28. Tabor, S., and Richardson, C. C. (1985) Proc. Natl. Acad. Sci. U. S. A. 82, 1074-1078
29. Sjöberg, B.-M., Hahne, S., Karlsson, M., Jörnvall, H., Göransson, M., and Uhlin, B. E. (1986) J. Biol. Chem. 261, 5658-5662
30. Sahlin, M., Sjöberg, B.-M., Backes, G., Loehr, T., and Sanders-Loehr, J. (1990) Biochem. Biophys. Res. Commun. 167, 813-818
31. Nordlund, P., Uhlin, U., Westergren, C., Joelson, T., Sjöberg, B.-M., and Eklund, H. (1989) FEBS Lett. 258, 251-254
32. Otwinowski, Z. (1993) in Proceedings of the CCP 4 Study Weekend: Data Collection and Processing, January 29-30, 1993 (Sawyer, L., Isaacs, N., Bailey, S. eds) pp. 56-62, SERC Daresbury Laboratory, England
33. Logan, D. T., Su, X. D., Regnström, K., Hajdu, J., Eklund, H., and Nordlund, P. (1996) Structure 4, 1053-1064
34. Tronrud, D. E., ten Eyck, L. F., and Matthews, B. W. (1987) Acta Crystallogr. A 43, 481-501
35. Brünger, A. T. (1992) Nature 355, 472-475
36. Engh, R. A., and Huber, R. (1991) Acta Crystallogr. A 47, 392-400
37. Lassmann, G., Thelander, L., and Gräslund, A. (1992) Biochem. Biophys. Res. Commun. 188, 879-887
38. Sahlin, M., Gräslund, A., and Ehrenberg, A. (1986) J. Magn. Reson. 67, 135-137
39. Bernhard, W. A., and Fouse, G. W. (1989) J. Magn. Reson. 82, 156-162
40. Sahlin, M., Petersson, L., Gräslund, A., Ehrenberg, A., Sjöberg, B.-M., and Thelander, L. (1987) Biochemistry 26, 5541-5548
41. Kelman, D. J., DeGray, J. A., and Mason, R. P. (1994) J. Biol. Chem. 269, 7458-7463
42. Sjöberg, B.-M., Reichard, P., Gräslund, A., and Ehrenberg, A. (1977) J. Biol. Chem. 252, 536-541
43. Merritt, E. A., and Murphy, M. E. P. (1994) Acta Crystallogr. D 50, 869-873
44. Hoganson, C. W., and Babcock, G. T. (1992) Biochemistry 31, 11874-11880
45. Gray, H., B. (1990) Aldrichimica Acta 23, 87-93
46. Isied, S. (1991) in Metal Ions in Biological Systems (Sigel, H., and Sigel, A., ed) Vol. 27, pp. 1-56, Marcel Dekker, Inc., New York
47. Ormö, M., Regnström, K., Wang, Z., Que, L., Jr., Sahlin, M., and Sjöberg, B.-M. (1995) J. Biol. Chem. 270, 6570-6576
48. Ekberg, M., Sahlin, M., Eriksson, M., and Sjöberg, B.-M. (1996) J. Biol. Chem. 271, 20655-20659