Nuclear-cytoplasmic shuttling of the focal contact protein, zyxin: A potential mechanism for communication between sites of cell adhesion and the nucleus

Journal of Cell Biology

Volumn 138, Issue 5, 1997, Pages 1139-1147

  Nix, David A ^a   Beckerle, Mary C ^a  

^a UNIVERSITY OF UTAH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CELL SURFACE RECEPTOR; GLUTATHIONE TRANSFERASE; HYBRID PROTEIN; INTEGRIN; PHOSPHOPROTEIN; UNCLASSIFIED DRUG; ZYXIN;

ANIMAL CELL; ARTICLE; CELL ADHESION; CELL COMMUNICATION; CELL NUCLEUS; CYTOPLASM; EMBRYO; EXTRACELLULAR MATRIX; FIBROBLAST; GENE EXPRESSION; NONHUMAN; PRIORITY JOURNAL; PROTEIN LOCALIZATION; RAT;

AMINO ACID SEQUENCE; ANIMALS; AVIAN PROTEINS; CELL ADHESION; CELL ADHESION MOLECULES; CELL LINE; CELL NUCLEUS; CHICKENS; CLONING, MOLECULAR; CONSERVED SEQUENCE; CYTOPLASM; FIBROBLASTS; GLUTATHIONE TRANSFERASE; LEUCINE; METALLOPROTEINS; MOLECULAR SEQUENCE DATA; NUCLEAR PROTEINS; POLYMERASE CHAIN REACTION; RATS; RECEPTORS, CELL SURFACE; RECOMBINANT FUSION PROTEINS; SEQUENCE ALIGNMENT; SIGNAL TRANSDUCTION; TRANSCRIPTION, GENETIC; ZINC FINGERS;

ANIMALIA;

EID: 0030967789     PISSN: 00219525     EISSN: None     Source Type: Journal    
DOI: 10.1083/jcb.138.5.1139     Document Type: Article

References (59)

1. Arber, S., G. Halder, and P. Caroni. 1994. Muscle LIM protein, a novel essential regulator of myogenesis, promotes myogenic differentiation. Cell. 79: 221-231.
2. Arber, S., J.J. Hunter, J.J. Ross, M. Hongo, G. Sansig, J. Borg, J. Perriard, K.R. Chien, and P. Caroni. 1997. MLP-deficient mice exhibit a disruption of cardiac cytoarchitectural organization, dilated cardiomyopathy, and heart failure. Cell. 88:393-403.
3. Baldwin, A.S., Jr. 1996. The NF-κ B and I κB proteins: new discoveries and insights. Annu. Rev. Immunol. 14:649-683.
4. Beckerle, M.C. 1986. Identification of a new protein localized at sites of cell-substrate adhesion. J. Cell Bioil. 103:1679-1687.
5. Beckerle, M.C. 1997. Zyxin: zinc fingers at sites of cell adhesion. Bioessays. In press.
6. Behrens, J., J.P. von Kries, M. Kuhl, L. Bruhn, D. Wedlich, R. Grosschedl, and W. Birchmeier. 1996. Functional interaction of β-catenin with the transcription factor LEF-1. Nature (Lond.). 382:638-642.
7. Bogerd, H.P., R.A. Fridell, R.E. Benson, J. Hua, and B.R. Cullen. 1996. Protein sequence requirements for function of the human T-cell leukemia virus type 1 Rex nuclear export signal delineated by a novel in vivo randomization-selection assay. Mol. Cell. Biol. 16:4207-4214.
8. Chen, Q., M.S. Kinch, T.H. Lin, K. Burridge, and R.L. Juliano. 1994. Integrin-mediated cell adhesion activates mitogen-activated protein kinases. J. Biol. Chem. 269:26602-26605.
9. Chun, J.S., and B.S. Jacobson. 1993. Requirement for diacylglycerol and protein kinase C in HeLa cell-substratum adhesion and their feedback amplification of arachidonic acid production for optimum cell spreading. Mol. Biol. Cell. 4:271-281.
10. Clark, E.A., and J.S. Brugge. 1993. Redistribution of activated pp60c-src to integrin-dependent cytoskeletal complexes in thrombin-stimulaled platelets. Mol. Cell. Biol. 13:1863-1871.
11. Clark, E.A., and J.S. Brugge. 1995. Integrins and signal transduction pathways: the road taken. Science (Wash. DC). 268:233-239.
12. Cobb, B.S., M.D. Schaller, T.H. Leu, and J.T. Parsons. 1994. Stable association of pp60src and pp59fyn with the focal adhesion-associated protein tyrosine kinase, pp125FAK. Mol. Cell. Biol. 14:147-155.
13. Crawford, A.W., and M.C. Beckerle. 1991. Purification and characterization of zyxin, an 82,000-daIlon component of adherens junctions. J. Biol. Chem. 266: 5847-5853.
14. Crawford, A.W., J.W. Michelsen, and M.C. Beckerle. 1992. An interaction between zyxin and α-actinin. J. Cell Biol. 116:1381-1393.
15. Crawford, A.W., J.D. Pino, and M.C. Beckerle. 1994. Biochemical and molecular characterization of the chicken cysteine-rich protein, a developmentally regulated LIM-domain protein that is associated with the actin cytoskeleton. J. Cell Biol. 124:117-127.
16. Eberhart, D.E., H.E. Malter, Y. Feng, and S.T. Warren. 1996. The fragile X mental retardation protein is a ribonucleoprotein containing both nuclear localization and nuclear export signals. Hum. Mol. Genet. 5:1083-1091.
17. Fischer, U., J. Huber, W.C. Bodens, I.W. Mattaj, and R. Luhrmann. 1995. The HIV-1 Rev activation domain is a nuclear export signal that accesses an export pathway used by specific cellular RNAs. Cell. 82:475-483.
18. Fridell, R.A., U. Fischer, R. Luhrmann, B.E. Meyer, J.L. Meinkoth, M.H. Malim, and B.R. Cullen. 1996a. Amphibian transcription factor IIIA proteins contain a sequence element functionally equivalent to the nuclear export signal of human immunodeficiency virus type 1 Rev. Proc. Natl. Acad. Sci. USA. 93:2936-2940.
19. Fridell, R.A., R.E. Benson, J. Hua, H.P. Bogerd, and B.R. Cullen. 1996b. A nulear role for the fragile X mental retardation protein. EMBO (Eur. Mol. Biol. Organ.) J. 15:5408-5414.
20. 2-terminal, leucine-rich short amino acid sequence, which acts as a nuclear export signal. J. Biol. Chem. 271:20024-20028.
21. Gertler, F.B., K. Niebuhr, M. Reinhard, J. Wehland, and P. Soriano. 1996. Mena, a relative of VASP and Drosophila Enabled, is implicated in the control of microfilament dynamics. Cell. 87:227-239.
22. Gill, G.N. 1995. The enigma of LIM domains. Structure (Lond.). 3:1285-1289.
23. Golsteyn, R.M., M.C. Beckerle, T. Koay, and E. Friederich. 1997. J. Cell Science. In press.
24. Görlich, D., and I.W. Mattaj. 1996. Nucleocytoplasmic transport. Science (Wash. DC). 271:1513-1518.
25. Gottardi, C.J., M. Arpin, A.S. Fanning, and D. Louvard. 1996. The junction-associated, ZO-1, localizes to the nucleus before the maturation and during the remodeling of cell-cell contacts. Proc. Natl. Acad. Sci. USA. 93:10779-10784.
26. Gumbiner, B.M. 1995. Signal transduction of β-catenin. Curr. Opin. Cell Biol. 7:634-640.
27. Gumbiner, B.M. 1996. Cell adhesion: the molecular basis of tissue architecture and morphogenesis. Cell. 84:345-357.
28. Harlow, E., and D. Lane. 1988. Antibodies: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY. 726 pp.
29. Hobert, O., J.W. Schilling, M.C. Beckerle, A. Ullrich, and B. Jallal. 1996. SH3 domain-dependent interaction of the proto-oncogene product Vav with the focal contact protein zyxin. Oncogene. 12:1577-1581.
30. Huber, O., C. Bierkamp, and R. Kemler. 1996a. Cadherins and catenins in development. Curr. Opin. Cell Biol. 8:685-691.
31. Huber, O., R. Korn, J. McLaughlin, M. Ohsugi, B.G. Herrmann, and R. Kemler. 1996b. Nuclear localization of β-catenin by interaction with transcription factor LEF-1. Mech. Dev. 59:3-10.
32. Kalderon, D., B.L. Roberts, W.D. Richardson, and A.E. Smith. 1984. A short amino acid sequence able to specify nuclear localization. Cell. 39:499-509.
33. Kim, F.J.. A.A. Beeche, J.J. Hunter, D.J. Chin, and T.J. Hope. 1996. Characterization of the nuclear export signal of human T-cell lymphotropic virus type 1 Rex reveals that nuclear export is mediated by position-variable hydrophobic interactions. Mol. Cell Biol. 16:5147-5155.
34. Lamarche, N., N. Tapon, L. Stowers, P.D. Burbelo, P. Aspenstrom, T. Bridges, J. Chant, and A. Hall. 1996. Rac and Cdc42 induce actin polymerization and G1 cell cycle progression independently of p65PAK and the JNK/SAPK MAP kinase cascade. Cell. 87:519-529.
35. Lewis, J.M., R. Baskaran, S. Taagepera, M.A. Schwartz, and J.Y.J. Wang. 1996. Integrin regulation of c-Abl tyrosine kinase activity and cytoplasmic-nuclear transport. Proc. Natl. Acad. Sci. USA. 93:15174-15179.
36. Macalma, T., J. Otte, M.E. Hensler, S.M. Bockholt, H.A. Louis, M. Kalff-Suske, K.H. Grzeschik, D. von der Ahe, and M.C. Beckerle. 1996. Molecular characterization of human zyxin. J. Biol. Chem. 271:31470-31478.
37. McClure, D.B., M.J. Hightower, and W.C. Topp. 1982. Effect of SV40 transformation of the growth factor requirements of the rat embryo cell line REF52 in serum free medium. In Growth of Cells in Hormonally Defined Media. G.H. Sato, A.B. Pardee, and D.A. Sirbasku, editors. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY. 345-364.
38. McNamee, H.P., D.E. Ingber, and M.A. Schwartz. 1993. Adhesion to fibronectin stimulates inositol lipid synthesis and enhances PDGF-induced inositol lipid breakdown. J. Cell Biol. 121:673-678.
39. Murphy, R., and S.R. Wente. 1996. An RNA-export mediator with an essential nuclear export signal. Nature (Lond.). 383:357-360.
40. Orsulic, S., and M. Peifer. 1996. An in vivo structure-function study of armadillo, the β-catenin homologue, reveals both separate and overlapping regions of the protein required for cell adhesion and for wingless signaling. J. Cell Biol. 134:1283-1300.
41. Otey, C.A. 1996. pp125FAK in the focal adhesion. Int. Rev. Cytol. 167:161-183.
42. Paine, P.L., and C.M. Feldherr. 1972. Nucleocytoplasmic exchange of macromolecules. Exp. Cell Res. 74:81-98.
43. Palmeri, D., and M.H. Malim. 1996. The human T-cell leukemia virus type 1 posttranscriptional trans-activator Rex contains a nuclear export signal. J. Virol. 70:6442-6445.
44. Pavalko, F.M., and K. Burridge. 1991. Disruption of the actin cytoskeleton after microinjection of proteolytic fragments of α-actinin. J. Cell Biol. 114:481-491.
45. Perez-Alvarado, G.C., C. Miles, J.W. Michelsen, H.A. Louis, D.R. Winge, M.C. Beckerle, and M.F. Summers. 1994. Structure of the COOH-terminal LIM domain from the cysteine rich protein CRP. Nat. Struct. Biol. 1:388-398.
46. Reinhard, M., K. Jouvenal, D. Tripier, and U. Walter. 1995. Identification, purification, and characterization of a zyxin-related protein that binds the focal adhesion and microfilament protein VASP (vasodilator-stimulated phosphoprotein). Proc. Natl. Acad. Sci. USA. 92:7956-7960.
47. Richards, S.A., K.M. Lounsbury, K.L. Carey, and I.G. Macara. 1996. A nuclear export signal is essential for the cytosolic localization of the Ran binding protein, RanBP1. J. Cell Biol. 134:1157-1168.
48. Sadler, I., A.W. Crawford, J.W. Michelsen, and M.C. Beckerle. 1992. Zyxin and cCRP: two interactive LIM domain proteins associated with the cytoskeleton. J. Cell Biol. 119:1573-1587.
49. Sambrook, J., E.F. Fritsch, and T. Maniatis. 1989. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY. 1.38-1.46.
50. Sanchez-Garcia, I., and T.H. Rabbitts. 1994. The LIM domain: a new structural motif found in zinc-finger-like proteins. Trends Genet. 10:315-320.
51. Schmeichel, K.L., and M.C. Beckerle. 1994. The LIM domain is a modular protein-binding interface. Cell. 79:211-219.
52. Schmeichel, K.L., and M.C. Beckerle. 1997. Molecular dissection of a LIM domain. Mol. Biol. Cell. 8:219-230.
53. Shimizu, Y., J.L. Mobley, L.D. Finkelstein, and A.S. Chan. 1995. A role for phosphatidylinositol 3-kinase in the regulation of β1 integrin activity by the CD2 antigen. J. Cell Biol. 131:1867-1880.
54. Stronach, B.E., S.E. Siegrist, and M.C. Beckerle. 1996. Two muscle-specific LIM proteins in Drosophila. J. Cell Biol. 134:1179-1195.
55. Vuori, K., and E. Ruoslahti. 1993. Activation of protein kinase C precedes α5 β1 integrin-mediated cell spreading on fibronectin. J. Biol Chem. 268:21459-21462.
56. Wang, L.F., S.Y. Miao, S.D. Zong, Y. Bai, and S.S. Koide. 1994. Gene encoding a mammalian epididymal protein. Biochem. Mol. Biol. Int. 34:1131-1136.
57. Wen, W., J.L. Meinkoth, R.Y. Tsien, and S.S. Taylor. 1995. Identification of a signal for rapid export of proteins from the nucleus. Cell. 82:463-473.
58. Wu, R.Y., and G.N. Gill. 1994. LIM domain recognition of a tyrosine-containing tight turn. J. Biol. Chem. 269:25085-25090.
59. Zhu, X., and R.K. Assoian. 1995. Integrin-dependent activation of MAP kinase: a link to shape-dependent cell proliferation. Mol. Biol. Cell. 6:273-282.