Coexpression of the B subunit of Shiga toxin 1 and EaeA from enterohemorrhagic Escherichia coli in Vibrio cholerae vaccine strains

Infection and Immunity

Volumn 65, Issue 6, 1997, Pages 2127-2135

  Butterton, Joan R ^a   Ryan, Edward T ^a   Acheson, David W K ^b   Calderwood, Stephen B ^a,c  

^a MASSACHUSETTS GENERAL HOSPITAL   (United States)

^b TUFTS MEDICAL CENTER   (United States)

^c HARVARD MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; SHIGA TOXIN;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANTIBODY RESPONSE; ANTIGEN EXPRESSION; ARTICLE; BETA CHAIN; CHROMOSOME INSERTION; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; IMMUNIZATION; IMMUNOGENICITY; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; RABBIT; TRANSCRIPTION REGULATION; TRANSLATION REGULATION; VIBRIO CHOLERAE;

EID: 0030961347     PISSN: 00199567     EISSN: None     Source Type: Journal    
DOI: 10.1128/iai.65.6.2127-2135.1997     Document Type: Article

References (54)

1. Acheson, D. W. K., S. B. Calderwood, S. A. Boyko, L. L. Lincicome, A. V. Kane, A. Donohue-Rolfe, and G. T. Keusch. 1993. Comparison of Shiga-like toxin I B-subunit expression and localization in Escherichia coli and Vibrio cholerae by using trc or iron-regulated promoter systems. Infect. Immun. 61:1098-1104.
2. Acheson, D. W. K., M. M. Levine, J. B. Kaper, and G. T. Keusch. 1996. Protective immunity to Shiga-like toxin I following oral immunization with Shiga-like toxin IB-subunit-producing Vibrio cholerae CVD 103-HgR. Infect. Immun. 64:355-357.
3. Beebakhee, G., M. Louie, J. De Azavedo, and J. Brunton. 1992. Cloning and nucleotide sequence of the eae gene homologue from enterohemorrhagic Escherichia coli serotype O157:H7. FEMS Microbiol. Lett. 91:63-68.
4. Bell, B. P., M. Goldoft, P. M. Griffin, M. A. Davis, D. C. Gordon, P. I. Tarr, C. A. Bartleson, J. H. Lewis, T. J. Barrett, J. G. Wells, R. Baron, and J. Kobayashi. 1994. A multistate outbreak of Escherichia coli O157:H7-associated bloody diarrhea and hemolytic uremic syndrome from hamburgers: the Washington experience. JAMA 272:1349-1353.
5. Boyd, B., S. Richardson, and J. Gariepy. 1991. Serological responses to the B subunit of Shiga-like toxin 1 and its peptide fragments indicate that the B subunit is a vaccine candidate to counter the action of the toxin. Infect. Immun. 59:750-757.
6. Butterton, J. R., D. T. Beattie, C. L. Gardel, P. A. Carroll, T. Hyman, K. P. Killeen, J. J. Mekalanos, and S. B. Calderwood. 1995. Heterologous antigen expression in Vibrio cholerae vector strains. Infect. Immun. 63:2689-2696.
7. Butterton, J. R., S. A. Boyko, and S. B. Calderwood. 1993. Use of the Vibrio choleras irgA gene as a locus for insertion and expression of heterologous antigens in cholera vaccine strains. Vaccine 11:1327-1335.
8. Butterton, J. R., and S. B. Calderwood. 1997. Attenuated Vibrio cholerae as live vectors for expression of foreign antigens, p. 379-385, In M. M. Levine, G. C. Woodrow, J. B. Kaper, and G. S. Cobon (ed.), New generation vaccines, 2nd ed. Marcel Dekker, Inc., New York, N.Y.
9. Calderwood, S. B., D. W. Acheson, M. B. Goldberg, S. A. Boyko, and R. A. Donohue. 1990. A system for production and rapid purification of large amounts of the Shiga toxin/Shiga-like toxin I B subunit. Infect. Immun. 58:2977-2982.
10. Coster, T. S., K. P. Killeen, M. K. Waldor, D. T. Beattie, D. R. Spriggs, J. R. Kenner, A. Trofa, J. C. Sadoff, J. J. Mekalanos, and D. N. Taylor. 1995. Safety, immunogenicity, and efficacy of live attenuated Vibrio choleras O139 vaccine prototype. Lancet 345:949-952.
11. Cray, W. C. J., E. Tokunaga, and N. F. Pierce. 1983. Successful colonization and immunization of adult rabbits by oral inoculation with Vibrio cholerae O1. Infect. Immun. 41:735-741.
12. Czerkinsky, C., A.-M. Svennerholm, M. Quiding, R. Jonsson, and J. Holmgren, 1991. Antibody-producing cells in peripheral blood and salivary glands after oral cholera vaccination of humans. Infect. Immun. 59:996-1001.
13. Donnenberg, M. S., and J. B. Kaper. 1991. Construction of an eae deletion mutant of enteropathogenic Escherichia coli by using a positive-selection suicide vector. Infect. Immun. 59:4310-4317.
14. Donnenberg, M. S., C. O. Tacket, S. P. James, G. Losonsky, J. P. Nataro, S. S. Wasserman, J. B. Kaper, and M. M. Levine. 1993. Role of the eaeA gene in experimental enteropathogenic Escherichia coli infection. J. Clin. Invest. 92:1412-1417.
15. Donnenberg, M. S., S. Tzipori, M. L. McKee, A. D. O'Brien, J. Alroy, and J. B. Kaper. 1993. The role of the eae gene of enterohemorrhagic Escherichia coli in intimate attachment in vitro and in a porcine model. J. Clin. Invest. 92:1418-1424.
16. Donohue-Rolfe, A., D. W. K. Acheson, A. V. Kane, and G. T. Keusch. 1989. Purification of Shiga toxin and Shiga-like toxins I and II by receptor analog affinity chromatography with immobilized IM glycoproiein and production of cross-reactive monoclonal antibodies. Infect. Immun. 57:3888-3893.
17. Donohue-Rolfe, A., M. A. Kelley, M. Bennish, and G. T. Keusch. 1986. Enzyme-linked immunosorbent assay for Shigella toxin. J. Clin. Microbiol. 24:65-68.
18. Donohue-Rolfe, A., G. T. Keusch, C. Edson, D. Thorley-Lawson, and M. Jacewicz. 1484. Pathogenesis of Shigella diarrhea. IX. Simplified high yield purification of Shigella toxin and characterization of subunit composition and function by the use of subunit-specific monoclonal and polyclonal antibodies. J. Exp. Med. 160:1767-1781.
19. Goldberg, M. B., S. A. Boyko, and S. B. Calderwood. 1991. Positive transcriptional regulation of an iron-regulated virulence gene in Vibrio cholerae. Proc. Natl. Acad. Sci. USA 88:1125-1129.
20. Goldberg, M. B., V. J. DiRita, and S. B. Calderwood. 1990. Identification of an iron-regulated virulence determinant in Vibrio cholerae, using TnphoA mutagenesis. Infect. Immun. 58:55-60.
21. Gordon, V. M., S. C. Whipp, H. W. Moon, A. D. O'Brien, and J. E. Samuel, 1992. An enzymatic mutant of Shiga-like toxin II variant is a vaccine candidate for edema disease of swine. Infect. Immun. 60:485-490.
22. Greatorex, J. S., and G. M. Thorne. 1994. Humoral immune responses to Shiga-like toxins and Eseherichia coli O157 lipopolysaccharide in hemolytic-uremic syndrome patients and healthy subjects. J. Clin. Microbiol. 32:1172-1178.
23. Guest, R. 1996. Four die in food poisoning outbreak in Japan. Br. Med. J. 313:187.
24. Haider, K., D. T. Beattie, K. L. Metcalfe, and K. P. Killeen. 1995. Immunogenicity of Vibrio cholerae vectors expressing Shigella sonnei O-antigen in rabbits, abstr. E-78. In Abstracts of the 95th General Meeting of the American Society for Microbiology 1995. American Society for Microbiology, Washington, D.C.
25. Harari, I., and R. Arnon. 1990. Carboxy-terminal peptides from the B subunit of Shiga toxin induce a local and parenteral protective effect. Mol. Immunol. 27:613-621.
26. Harari, I., A. Donohue-Rolfe, G. Keusch, and R. Arnon. 1983. Synthetic peptides of Shiga toxin B subunit induce antibodies which neutralize its biologic activity. Infect. Immun. 56:1018-1624.
27. Hovde, C. J., S. B. Calderwood, J. J. Mekalanos, and R. J. Collier. 1988. Evidence that glutamic acid 167 is an active-site residue of Shiga-like toxin I. Proc. Natl. Acad. Sci. USA 85:2508-2572.
28. Iwanaga, M., K. Yamamoto, N. Higa, Y. Ichinose, N. Nakasone, and M. Tanabe. 1986. Culture conditions for stimulating cholera toxin production by Vibrio cholerae O1 El Tor. Microbiol. Immunol. 30:1075-1083.
29. Jerse, A. E., K. G. Gicquelais, and J. B. Kaper. 1991. Plasmid and chromosomal elements involved in the pathogenesis of attaching and effacing Escherichia coli. Infect. Immun. 59:3869-3875.
30. Jerse, A. E., and J. B. Kaper. 1991. The eae gene of enteropathogenic Escherichia coli encodes a 94-kilodalton membrane protein, the expression of which is influenced by the EAF plasmid. Infect. Immun. 59:4302-4309.
31. Jerse, A. E., J. Yu, B. D. Tall, and J. B. Kaper. 1990. A genetic locus of enteropathogenic Escherichia coli necessary for the production of attaching and effacing lesions on tissue culture cells. Proc. Natl. Acad. Sci. USA 87:7839-7843.
32. Levine, M. M., E. J. Bergquist, D. R. Nalin, D. H. Waterman, R. B. Hornick, C. R. Young, S. Sotman, and B. Rowe. 1978. Escherichia coli strains that cause diarrhea but do not produce heat-labile or heat-stable enterotoxins and are non-invasive. Lancet i:1119-1122.
33. Levine, M. M., R. E. Black, M. L. Clements, L. Cisneros, D. R. Nalin, and C. R. Young. 1981. Duration of infection-derived immunity to cholera. J. Infect. Dis. 143:818-820.
34. Levine, M. M., D. Herrington, G. Losonsky, B. Tall, J. B. Kaper, J. Ketley, C. O. Tacket, and S. Cryz. 1988. Safety, immunogenicity, and efficacy of recombinant live oral cholera vaccines, CVD 103 and CVD 103-HgR. Lancet 11:467-470.
35. Levine, M. M., J. McEwen, G. Losonsky, M. Reymann, I. Harari, J. E. Brown, D. N. Taylor, A. Donohue-Rolfe, D. Cohen, M. Bennish, Y. L. Lim, and R. Arnon. 1992. Antibodies to Shiga holotoxin and to two synthetic peptides of the B subunit in sera of patients with Shigella dysenteriae 1 dysentery. J. Clin. Microbiol. 30:1636-1641.
36. Louie, M., C. S. De Azavedo, M. Y. C. Handelsman, C. G. Clark, B. Ally, M. Dytoc, P. Sherman, and J. Brunton. 1993. Expression and characterization of the eaeA gene product of Escherichia coli serotype O157:H7. Infect. Immun. 61:4085-4092.
37. McDaniel, T. K., K. G. Jarvis, M. S. Donnenberg, and J. B. Kaper. 1995. A genetic locus of enterocyte effacement conserved among diverse enterobacterial pathogens. Proc. Natl. Acad. Sci. USA 92:1664-1668.
38. McKee, M. L., A. R. Melton-Celsa, R. A. Moxley, D. H. Francis, and A. D. O'Brien. 1995. Enterohemorrhagic Escherichia coli O157:H7 requires intimin to colonize the gnotobiotic pig intestine and to adhere to HEp-2 cells. Infect. Immun. 63:3739-3744.
39. McKee, M. L., and A. D. O'Brien. 1996. Truncated enterohemorrhagic Escherichia coli (EHEC) 0157:H7 intimin (EacA) fusion proteins promote adherence of EHEC strains to HEp-2 cells. Infect. Immun. 64:2225-2233.
40. Mekalanos, J. J., D. J. Swartz, G. D. Pearson, N. Harford, F. Groyne, and M. de Wilde. 1983. Cholera toxin genes: nucleotide sequence, deletion analysis and vaccine development. Nature 306:551-557.
41. Mullett, C., D. W. K. Acheson, L.-Y. Tseng, and E. Boedeker. 1994. High titer serum antibody against the B subunit of Shiga-like toxin I protects in a model of enterohemorrhagic Escherichia coli (EHEC) colitis. Gastroenterology 106:A740.
42. Owen, R. L., N. F. Pierce, R. T. Apple, and W. C. Cray. 1986. M cell transport of Vibrio cholerae from the intestinal lumen into Peyer's patches: a mechanism for antigen sampling and for microbial transepithelial migration. J. Infect. Dis. 153:1108-1118.
43. Roberts, A., G. D. Pearson, and J. J. Mekalanos. 1992. Cholera vaccine strains derived from a 1991 Peruvian isolate of Vibrio cholerae and other El Tor strains. In Proceedings of the 28th Joint Conference, U.S.-Japan Cooperative Medical Science Program on Cholera and Related Diarrheal Diseases. National Institutes of Health, Bethesda, MD.
44. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
45. Svennerholm, A.-M., D. A. Sack, J. Holmgren, and P. K. Bardhan. 1982. Intestinal antibody responses after immunisation with cholera B subunit. Lancet 1:305-308.
46. Swinbanks, D. 1996. Outbreak of E. coli infection in Japan renews concerns. Nature 382:290.
47. Tarr, P. I. 1995. Escherichia coli O157:H7: clinical, diagnostic, and epidemiological aspects of human infection. Clin. Infect. Dis. 20:1-10.
48. Tautz, D., and M. Renz. 1983. An optimized freeze-squeeze method for the recovery of DNA fragments. Anal. Biochem. 132:14-19.
49. Taylor, D. N., K. P. Killeen, D. C. Hack, J. R. Kenner, T. S. Coster, D. T. Beattie, J. Ezzell, T. Hyman, A. Trofo, M. H. Sjogren, A. Friedlander, J. J. Mekalanos, and J. C. Sadoff. 1994. Development of a live, oral attenuated vaccine against El Tor cholera. J. Infect. Dis. 170:1518-1523.
50. Tesh, V. L., and A. D. O'Brien. 1991. The pathogenic mechanisms of Shiga toxin and the Shiga-like toxins. Mol. Microbiol. 5:1817-1822.
51. Tzipori, S., F. Gunzer, M. S. Donnenberg, L. De Montigny, J. B. Kaper, and A. Donohue-Rolfe. 1995. The role of the eaeA gene in diarrhea and neurological complications in a gnotobiotic piglet model of enterohemorrhagic Escherichia coli infection. Infect. Immun. 63:3621-3627.
52. Wadolkowski, E. A., L. M. Sung, J. A. Burris, J. E. Samuel, and A. D. OBrien. 1990. Acute renal tubular necrosis and death of mice orally infected with Escherichia coli strains that produce Shiga-like toxin type II. Infect. Immun. 58:3959-3965.
53. Waldor, M. K., and J. J. Mekalanos. 1994. Emergence of a new cholera pandemic: molecular analysis of virulence determinants in Vibrio cholerae O139 and development of a live vaccine prototype. J. Infect. Dis. 170:278-283,
54. Yu, J., and J. B. Kaper. 1992. Cloning and characterization of the eae gene of enterohemorrhagic Escherichia coli O157:H7. Mol. Microbiol. 6:411-417.