Near-zero background cloning of PCR products

BioTechniques

Volumn 23, Issue 3, 1997, Pages 412-418

  Zeng, Qiandong ^a   Eidsness, Marly K ^a   Summers, Anne O ^a  

^a UNIVERSITY OF GEORGIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; GENETIC ANALYSIS; HUMAN; MOLECULAR BIOLOGY; MOLECULAR CLONING; NONHUMAN; POLYMERASE CHAIN REACTION; PROTEIN ANALYSIS; PROTEIN EXPRESSION;

EID: 0030961036     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/97233bm13     Document Type: Article

References (19)

1. Ausubel, F.M., R. Brent, R.E. Kingston, D.D. Moore, J.G. Siedman, J.A. Smith and K. Struhl (Eds.). 1993. Current Protocols in Molecular Biology. John Wiley & Sons, New York.
2. Bernard, P. 1996. Positive selection of recombinant DNA by CcdB. BioTechniques 21:320-323.
3. Bernard, P., P. Gabant, E.M. Bahassi and M. Couturier. 1994. Positive selection vectors using the F plasmid ccdB killer gene. Gene 148:71-74.
4. De Backer, O., P. Chomez and E. De Plaen. 1994. Positive selection of recombinant plasmids based on the EcoK restriction activity of Escherichia call K-12. Gene 150:197-198.
5. Dieffenbach, C.W. and G.S. Dvejsler (Eds.). 1995. PCR Primer: A Laboratory Manual. CSH Laboratory Press, Cold Spring Harbor, NY.
6. Henrich, B. and R. Plapp. 1986. Use of the lysis gene of bacteriophage φX174 for the construction of a positive selection vector. Gene 42:345-349.
7. Henrich, B. and B. Schmidtberger. 1995. Positive selection vector with enhanced lytic potential based on a variant of φX174 phage gene E. Gene 154:51-54.
8. Kaufman, D.L. and G.A. Evans. 1990. Restriction endonuclease cleavage at the termini of PCR products. BioTechniques 9:304-306.
9. Kuhn, I., F.H. Stephenson, H.W. Boyer and P.J. Greene. 1986. Positive-selection vectors utilizing lethality of the EcoRI endonuclease. Gene 42:253-263.
10. Lee, J. and S.M. Weissman. 1992. Construction of lambda gt103, a derivative of phage lambda gt10 that has unique EcoKI, NotI, SacI and SpeI sites and retains positive selection for recombinants. Gene 120:85-88.
11. Liu, Z.-g. and L.M. Schwartz. 1992. An efficient method for blunt-end ligation of PCR products. BioTechniques 12:28-30.
12. Loukianov, E.V. and S. Olsnes. 1993. New cloning opportunities with the use of noncomplementary SfiI adaptors. BioTechniques 15:980-982.
13. Sambrook, J., E.F. Fritsch and T. Maniatis. 1989. Molecular Cloning: A Laboratory Manual, 2nd ed. CSH Laboratory Press, Cold Spring Harbor, NY.
14. Saris, P.E.J. and L. Paulin. 1990. Easy selection of recombinant clones. BioTechniques 9:694-697.
15. Scharf, S.J., G.T. Horn and H.A. Erlich. 1986. Direct cloning and sequence analysis of enzymatically amplified genomic sequences. Science 233:1076-1078.
16. Shepard, A.R. and N.L. Eberhardt. 1992. A simple step to reduce background in E. coli transformations of blunt-ended plasmid ligation products. BioTechniques 13:40-42.
17. Summers, A.O. 1992. Untwist and shout: a heavy metal responsive transcriptional regulator. J. Bacteriol. 174:3097-3101.
18. Trudel, P., S. Provost, B. Massie, P. Chartrand and L. Wall. 1996. pGATA: a positive selection vector based on the toxicity of the transcription factor GATA-1 to bacteria. BioTechniques 20:684-693.
19. Vernet, T., P.C.K. Lau, S.A. Narang and L.P. Visentin. 1985. A direct-selection vector derived from pColE3-CA38 and adapted for foreign gene expression. Gene 34:87-93.