Influence of different rol gene products on the chain length of Shigella dysenteriae type 1 lipopolysaccharide O antigen expressed by Shigella flexneri carrier strains

Journal of Bacteriology

Volumn 179, Issue 7, 1997, Pages 2421-2425

  Klee, Silke R ^a,b   Tzschaschel, Barbara D ^a,c   Timmis, Kenneth N ^a   Guzmán, Carlos A ^a  

^a HELMHOLTZ CENTRE FOR INFECTION RESEARCH   (Germany)

^b MAX PLANCK INSTITUTE FOR MOLECULAR GENETICS   (Germany)

^c INSTITUTE OF EPIDEMIOLOGY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIUM LIPOPOLYSACCHARIDE; GENE PRODUCT; O ANTIGEN;

ANTIGEN EXPRESSION; ARTICLE; BACTERIAL GENE; ESCHERICHIA COLI K 12; GENE EXPRESSION REGULATION; NONHUMAN; PRIORITY JOURNAL; SHIGELLA DYSENTERIAE; SHIGELLA FLEXNERI;

BACTERIA (MICROORGANISMS); ESCHERICHIA; ESCHERICHIA COLI K12; SHIGELLA DYSENTERIAE; SHIGELLA FLEXNERI;

EID: 0030956373     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.179.7.2421-2425.1997     Document Type: Article

References (38)

1. Attridge, S. R., D. Daniels, J. K. Morona, and R. Morona. 1990. Surface co-expression of Vibrio cholerae and Salmonella typhi O-antigens on Ty21a clone EX210. Microb. Pathog. 8:177-188.
2. Bastin, D. A., L. K. Romana, and P. R. Reeves. 1991. Molecular cloning and expression in Escherichia coli K-12 of the rfb gene cluster determining the O antigen of an E. coli O111 strain. Mol. Microbiol. 5:2223-2231.
3. Bastin, D. A., G. Stevenson, P. K. Brown, A. Haase, and P. R. Reeves. 1993. Repeat unit polysaccharides of bacteria: a model for polymerization resembling that of ribosomes and fatty acid synthetase, with a novel mechanism for determining chain length. Mol. Microbiol. 7:725-734.
4. Batchelor, R. A., P. Alifano, E. Biffali, S. I. Hull, and R. A. Hull. 1992. Nucleotide sequences of the genes regulating O-polysaccharide antigen chain length (rot) from Escherichia coli and Salmonella typhimurium: protein homology and functional complementation. J. Bacteriol. 174:5228-5236.
5. Batchelur, R. A., G. E. Haraguchi, R. A. Hull, and S. I. Hull. 1991. Regulation by a novel protein of the bimodal distribution of lipo polysacch aride in the outer membrane of Escherichia coli. J. Bacteriol. 173:5699-5704.
6. Bennish, M. L., J. R. Harris, B. J. Wojtyniak, and M. Struelens. 1990. Death in shigellosis: incidence and risk factors in hospitalized patients. J. Infect. Dis. 161:500-506.
7. Brown, P. K., L. K. Romana, and P. R. Reeves. 1991. Cloning of the rfb gene cluster of a group C2 Salmonella strain: comparison with the rfb regions of groups B and D. Mol. Microbiol. 5:1873-1881.
8. Carlomagno, M. S., L. Chiariotti, P. Alifano, A. G. Nappo, and C. B. Bruni. 1988. Structure and function of the Salmonella typhimurium and Escherichia I coli K-12 histidine operons. J. Mol. Biol. 203:585-606.
9. Fält, I. C., D. Mills, E. K. H. Schweda, K. N. Timmis, and A. A. Lindberg. 1996. Construction of recomhinant aroA salmonellae stably producing the Shigella dysenteriae serotype 1 O-antigen and structural characterization of the Salmonella/Shigella hybrid LPS. Microb. Pathog. 20:11-30.
10. Fält, I. C., E. K. H. Schweda, S. Klee, M. Singh, E. Floderus, K. N. Timmis, and A. A. Lindberg. 1995. Expression of Shigella dysenteriae type 1 O-anti- f genie polysaccharide by Shigella flexneri aroD vaccine candidates and different S. flexneri serotypes. J. Bacteriol. 177:5310-5315.
11. Forrest, B. D., and J. T. LaBrooy. 1991. In vivo evidence of immunological masking of the Vibrio choterae O antigen of a hybrid Salmonella typhi Ty21aVibrio cholerae oral vaccine in humans. Vaccine 9:515-520.
12. Goldman, R. C., and F. Hunt. 1990. Mechanism of O-antigen distribution in lipopolysaccharide. J. Bacteriol. 172:5352-5359.
13. Grossman, N., M. A. Schmetz, J. Foulds, E. N. Klima, V. Jiminez, L. L. Leive, and K. A. Joiner. 1987. Lipopolysaccharide size and distribution determine serum resistance in Salmonella montevideo. J. Bacteriol. 169:856-863.
14. Guzmán, C. A., R. M. Brownlie, J. Kadurugamuwa, M. J. Walker, and K. N. Timmis. 1991. Antibody responses in the lungs of mice following oral immunization with Salmonella typhimurium aroA and invasive Escherichia coli strains expressing the filamentous hemagglutinin of Bordetella pertussis. Infect. Immun. 59:4391-4397.
15. Hitchcock, P. J., and T. M. Brown. 1983. Morphological heterogeneity among Salmonella lipopolysaccharide chemotypes in silver-stained polyacrylamide geis. J. Bacteriol. 154:269-277.
16. Jann, K., and B. Jann. 1984. Structure and biosynthesis of O-antigens, p. 118-186. In E. T. Rietschel (ed.), Handbook of endotoxin, vol. 1. Chemistry of endotoxin. Elsevier, Amsterdam, The Netherlands.
17. Joiner, K. A., N. Grossman, M. Schmetz, and L. Leive. 1986. C3 binds preferentially to long-chain lipopolysaccharide during alternative pathway activation by Salmonella Montevideo. J. Immunol. 136:710-715.
18. Karnell, A., B. A. D. Stocker, S. Katakura, F. P. Reinholt, and A. A. Lindberg. 1992. Live oral auxotrophic Shigella flexneri SFL124 vaccine with a deleted aroD gene: characterization and monkey protection studies. Vaccine 10:389-394.
19. Kessler, A., P. K. Brown, L. K. Romana, and P. R. Reeves. 1991. Molecular cloning and genetic characterization of the rfb region from Yersinia pseudotuberculosis serovar IIA, which determines the formation of the 3,6-dideoxyhexose anequose. J. Gen. Microbiol. 137:2689-2695.
20. Klee, S. R., B. D. Tzschaschel, I. Fält, A. Kärnell, A. A. Lindberg, K. N. Timmis, and C. A. Guzmán. Live attenuated vaccine candidate against Shigella dysenteriae type 1: construction and characterization. Submitted for publication.
21. Klee, S. R., B. D. Tzschaschel, M. Singh, I. Fait, A. A. Lindberg, K. N. Timmis, and C. A. Guzmán. Construction and characterization of genetically-marked bivalent anti-Shigella dysenteriae l and anti-Shigella flexneri Y live vaccine candidates. Microb. Pathog., in press.
22. Klena, J. D., E. Pradel, and C. A. Schnaitman. 1992. Comparison of lipopolysaccharide biosynthesis genes rfaK, rfaL, ifaY, and rfaZ of Escherichia coli K-12 and Salmonella typhimurium. J. Bacteriol. 174:4746-4752.
23. Klena, J. D., and C. A. Schnaitman. 1993. Function of the rfb gene cluster and the rfe gene in the synthesis of O antigen by Shigella dysenteriae 1. Mol. Microbiol. 9:393-402.
24. Laemmli, U. K. 1970. Cleavage of structural protein during the assembly of the head of bacteriophage T4. Nature 227:680-685.
25. Macpherson, D. F., R. Morona, D. W. Beger, K.-C. Cheah, and P. A. Manning. 1991. Genetic analysis of the rfb region of Shigella flexneri encoding the Y serotype O-antigen specificity. Mol. Microbiol. 5:1491-1499.
26. Mäkelä, P. H., and B. A. D. Stocker. 1984. Genetics of lipopolysaccharides, p. 59-137. In E. T. Rietschel (ed.), Handbook of endotoxin, vol. I. Chemistry of endotoxin. Eisevier, Amsterdam, The Netherlands.
27. Mills, S. D., T. Sekizaki, M. I. Gonzalez-Carrero, and K. N. Timmis. 1988. Analysis and genetic manipulation of Shigella virulence determinants for vaccine development. Vaccine 6:116-122.
28. Morona, R., M. Mavris, A. Fallarino, and P. A. Manning. 1994. Characterization of the rfc region of Shigella flexneri. J. Bacteriol. 176:733-747.
29. Morona, R., L. Van Den Bosch, and P. A. Manning. 1995. Molecular, genetic, and topological characterization of O-antigen chain length regulation in Shigella flexneri. J. Bacteriol. 177:1059-1068.
30. O'Callaghan, D., and A. Charbit. 1990. High efficiency transformation of Salmonella typhimurium and Salmonella typhi by electroporation. Mol. Gen. Genet. 223:156-158.
31. Stevenson, G., A. Kessler, and P. R. Reeves. 1995. A plasmid-borne O-antigen chain length determinant and its relationship to other chain length determinants. FEMS Microbiol. Lett. 125:25-30.
32. Sturm, S., and K. N. Timmis. 1986. Cloning of the rfb gene region of Shigella dysenteriae 1 and construction of an rfb-rfp gene cassette for the development of lipopolysaccharide-based live anti-dysentery vaccines. Microb. Pathog. 1:289-297.
33. Tsai, C.-M., and C. E. Frasch. 1982. A sensitive silver stain for detecting lipopolysaccharides in poly aery lamidc gels. Anal. Biochem. 119:115-119.
34. Tzschaschel, B. D., S. R. Klee, V. de Lorenzo, K. N. Timmis, and C. A. Guzmán. 1996. Towards a vaccine candidate against Shigella dysenteriae 1: expression of the Shiga toxin B-subunil in an attenuated Shigella flexneri aroD carrier strain. Microb. Pathog. 21:277-288.
35. Viret, J.-F., S. J. Cryz, and D. Favre. 1996. Expression of Shigella sonnei lipopolysaccharide in Vibrio choterae. Mol. Microbiol. 19:949-963.
36. Watanabe, H., and K. N. Timmis. 1984. A small plasmid in Shigella dysenteriae 1 specifies one or more functions essential for O antigen production and bacterial virulence. Infect. Immun. 43:391-396.
37. Westphal, O., and K. Jann. 1965. Bacterial lipopolysaccharides: extraction with phenol-water and further applications of the procedure. Methods Carbohydr. Chcm. 5:83-91.
38. Woodcock, D. M., P. J. Crowther, J. Doherry, S. Jefferson, E. DeCruz, M. Noyer-Weidner, S. S. Smith, M. Z. Michael, and M. W. Graham. 1989. Quantitative evaluation of Escherichia coli host strains for tolerance to cytosine methylation in plasmid and phage recombinants. Nucleic Acids Res. 17:3469-3478.