Molecular analysis of the Rhodococcus sp. strain H1 her gene and characterization of its product, a heroin esterase, expressed in Escherichia coli

Applied and Environmental Microbiology

Volumn 63, Issue 5, 1997, Pages 2062-2066

  Rathbone, Deborah A ^a   Holt, Peter John ^a   Lowe, Christopher R ^a   Bruce, Neil C ^a  

^a UNIVERSITY OF CAMBRIDGE   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

DIAMORPHINE ESTERASE; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; CHEMICAL STRUCTURE; ENZYME ISOLATION; ENZYME PURIFICATION; ENZYME STRUCTURE; ESCHERICHIA COLI; RHODOCOCCUS; STRAIN DIFFERENCE;

ESCHERICHIA COLI; RHODOCOCCUS; RHODOCOCCUS SP.;

EID: 0030954910     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/aem.63.5.2062-2066.1997     Document Type: Article

References (24)

1. Ausubel, F. M., R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith, and K. Struhl (ed.). 1945. Current protocols in molecular biology, 2nd ed. John Wiley & Sons Inc., New York, N.Y.
2. Bruce, N. C., D. A. Caswell, C. E. French, A. M. Hailes, M. T. Long, and D. L. Willey. 1994. Towards engineering pathways for the synthesis of analgesics and antitussives. Ann. N.Y. Acad. Sci. 721:85-99.
3. Bruce, N. C., D. L. Willey, A. F. W. Coulson, and J. Jeffery. 1994. Bacterial morphine dehydrogenase further defines a distinct superfamily of oxidoreductases with diverse functional activities. Biochem. J. 299:805-811.
4. Bruce, N. C., C. J. Wilmot, K. N. Jordan, L. D. Gray Stephens, and C. R. Lowe. 1991. Microbial degradation of the morphine alkaloids: purification and characterisation of morphine dehydrogenase from Pseudomonas putida M10. Biochem. J. 274:875-880.
5. Bruce, N. C., C. J. Wilmot, K. N. Jordan, A. E. Trebilcock, L. D. Gray Stephens, and C. R. Lowe. 1940. Microbial degradation of the morphine alkaloids: identification of morphinone as an intermediate in the metabolism of morphine by Pseudomonas putida M10. Arch. Microbiol. 154:465-470.
6. Cameron, G. W. W., K. N. Jordan, P.-J. Holt, P. B. Baker, C. R. Lowe, and N. C. Bruce. 1994. Identification of a heroin esterase in Rhodococcus sp. strain H1. Appl. Environ. Microbiol. 60:3881-3883.
7. Feller, G., M. Thiry, and C. Gerday. 1991. Nucleotide sequence of the lipase gene lip2 from the antarctic psychrotroph moraxella TA144 and site-specific mutagenesis of the conserved serine and histidine residues. DNA Cell Biol. 10:381-388.
8. Genetics Computer Group. 1991. Program manual for the GCG package, version 8, Genetics Computer Group, Madison, Wis.
9. Hart, S., R. Kirby, and D. R. Woods. 1990. Structure of a Rhodococcus gene encoding pigment production in Escherichia coli. J. Gen. Microbiol. 136: 1357-1363.
10. Holt, P.-J. 1996. Ph.D. thesis. Institute of Biotechnology, University of Cambridge, Cambridge, United Kingdom.
11. Holt, P.-J., N. C. Bruce, and C. R. Lowe. 1996. A bioluminescent assay for heroin and its metabolites. Anal. Chem. 68:1877-1882.
12. Holt, P.-J., L. D. Gray Stephens, N. C. Bruce, and C. R. Lowe. 1995. An electrochemical opiate assay. Biosensors Bioclectronics. 10:517-526.
13. Raibaud, A., M. Zalacain, T. G. Holt, R. Tizard, and C. J. Thompson. 1991. Nucleotide sequence analysis reveals linked N-acetyl hydrolase, thioesterase, transport, and regulatory genes encoded by the bialaphos biosynthetic gene cluster of Streptomyces hygroscopicus. J. Bacteriol. 173:4454-4463.
14. Rathbone, D. A., P.-J. Holt, N. C. Bruce, and C. R. Lowe. 1996. The use of recombinant DNA technology in the design of a highly specific heroin sensor. Ann. N.Y. Acad. Sci. 782:534-543.
15. Rathbone, D. A., P.-J. Holt, C. R. Lowe, and N. C. Bruce. 1996. The use of a novel recombinant heroin esterase in the development of an illicit drugs biosensor. Ann. N.Y. Acad. Sci. 799:90-96.
16. Salvi, S., M. Trieni, L. Lanfaloni, and C. L. Pon. 1994. The cloning and characterization of the gene encoding an esterase from Spirulina platensis. Mol. Gen. Genet. 243:124-126.
17. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1489. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
18. Schirmer, A., and D. Jendrossek. 1994. Molecular characterization of the extracellular Poly(3-hydroxyoctanoic acid) [poly(3-hydroxy)] depolymerase gene of Pseudomonas fluorescens GK13 and of its gene product. J. Bacteriol. 176:7065-7073.
19. Schwartz, D., R. Alijah, B. Nussbaumer, S. Pelzer, and W. Wohlleben. 1996. The peptide synthetase gene phsA from Streptomyces viridochromogenes is not juxtaposed with other genes involved in nonribosomal biosynthesis of peptides. Appl. Environ. Microbiol. 62:570-577.
20. Sobek, H., and H. Görisch. 1988. Purification and characterisation of a heat-stable esterase from the thermoacidophilic archaebacteria Sulpholobus acidocalderius. Biochem. J. 250:435-458.
21. Studier, F. W., and B. A. Moffatt. 1986. Use of bacteriophage T7 RNA polymerase to direct selective high level expression of cloned genes. J. Mol. Biol. 189:113-130.
22. Walker, C. H., and M. I. Mackness. 1983. Esterases: problems of identification and classification. Biochem. Pharm. 32:3265-3269.
23. Willey, D. L., D. A. Caswell, C. R. Lowe, and N. C. Bruce. 1993. Nucleotide sequence and overexpression of morphine dehydrogenase, a plasmid encoded acne from Pseudomonas putida M10. Biochem. J. 290:539-544.
24. Yanisch-Perron, C., J. Vieira, and J. Messing. 1985. Improved M13 phage cloninc vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33:103-119.