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Volumn 275, Issue 5306, 1997, Pages 1652-1654

A member of the frizzled protein family mediating axis induction by Wnt- 5A

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; EMBRYO; EMBRYO AXIS; EMBRYO DEVELOPMENT; NONHUMAN; PRIORITY JOURNAL; RECEPTOR BINDING; SIGNAL TRANSDUCTION; XENOPUS LAEVIS;

EID: 0030941498     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.275.5306.1652     Document Type: Article
Times cited : (424)

References (36)
  • 1
    • 0024972122 scopus 로고
    • C. R. Vinson, S. Conover, P. N. Adler, Nature 338, 263 (1989); W.-J. Park, J. C. Liu, P. N. Adler, Mech. Dev. 45, 127 (1995).
    • (1989) Nature , vol.338 , pp. 263
    • Vinson, C.R.1    Conover, S.2    Adler, P.N.3
  • 3
    • 0026476157 scopus 로고
    • S. D. Chan et al., J. Biol. Chem. 267, 25202 (1992); Z.-Y. Zhao, C. C. Lee, A. Baldini, C. T. Caskey, Genomics 27, 370 (1995); H. Sawa, L. Lobel, H. R. Horvitz, Genes Dev. 10, 2189 (1996).
    • (1992) J. Biol. Chem. , vol.267 , pp. 25202
    • Chan, S.D.1
  • 4
    • 0029046712 scopus 로고
    • S. D. Chan et al., J. Biol. Chem. 267, 25202 (1992); Z.-Y. Zhao, C. C. Lee, A. Baldini, C. T. Caskey, Genomics 27, 370 (1995); H. Sawa, L. Lobel, H. R. Horvitz, Genes Dev. 10, 2189 (1996).
    • (1995) Genomics , vol.27 , pp. 370
    • Zhao, Z.-Y.1    Lee, C.C.2    Baldini, A.3    Caskey, C.T.4
  • 5
    • 0029814396 scopus 로고    scopus 로고
    • S. D. Chan et al., J. Biol. Chem. 267, 25202 (1992); Z.-Y. Zhao, C. C. Lee, A. Baldini, C. T. Caskey, Genomics 27, 370 (1995); H. Sawa, L. Lobel, H. R. Horvitz, Genes Dev. 10, 2189 (1996).
    • (1996) Genes Dev. , vol.10 , pp. 2189
    • Sawa, H.1    Lobel, L.2    Horvitz, H.R.3
  • 7
    • 0029994517 scopus 로고    scopus 로고
    • P. Bhanot et al., Nature 382, 225 (1996).
    • (1996) Nature , vol.382 , pp. 225
    • Bhanot, P.1
  • 8
    • 0026772731 scopus 로고
    • R. Nusse and H. E. Varmus, Cell 69, 1073 (1992); A. P. McMahon, Trends Genet. 8, 236 (1992).
    • (1992) Cell , vol.69 , pp. 1073
    • Nusse, R.1    Varmus, H.E.2
  • 9
    • 0026703887 scopus 로고
    • R. Nusse and H. E. Varmus, Cell 69, 1073 (1992); A. P. McMahon, Trends Genet. 8, 236 (1992).
    • (1992) Trends Genet. , vol.8 , pp. 236
    • McMahon, A.P.1
  • 11
    • 0024435879 scopus 로고    scopus 로고
    • A. P. McMahon and R. T. Moon, Cell 58, 1075 (1989); W. C. Smith and R. M. Harland, ibid. 67, 753 (1991); S. Sokol, J. L. Christian, R. T. Moon, D. A. Melton, ibid., p. 741; A. Chakrabarti, G. Matthews, A. Colman, L. Dale, Development 115, 355 (1992); S. L. Wolda, C. J. Moody, R. T. Moon, Dev. Biol. 155, 46 (1993).
    • (1989) Cell , vol.58 , pp. 1075
    • McMahon, A.P.1    Moon, R.T.2
  • 12
    • 0025932959 scopus 로고
    • A. P. McMahon and R. T. Moon, Cell 58, 1075 (1989); W. C. Smith and R. M. Harland, ibid. 67, 753 (1991); S. Sokol, J. L. Christian, R. T. Moon, D. A. Melton, ibid., p. 741; A. Chakrabarti, G. Matthews, A. Colman, L. Dale, Development 115, 355 (1992); S. L. Wolda, C. J. Moody, R. T. Moon, Dev. Biol. 155, 46 (1993).
    • (1991) Cell , vol.67 , pp. 753
    • Smith, W.C.1    Harland, R.M.2
  • 13
    • 0024435879 scopus 로고    scopus 로고
    • A. P. McMahon and R. T. Moon, Cell 58, 1075 (1989); W. C. Smith and R. M. Harland, ibid. 67, 753 (1991); S. Sokol, J. L. Christian, R. T. Moon, D. A. Melton, ibid., p. 741; A. Chakrabarti, G. Matthews, A. Colman, L. Dale, Development 115, 355 (1992); S. L. Wolda, C. J. Moody, R. T. Moon, Dev. Biol. 155, 46 (1993).
    • Cell , pp. 741
    • Sokol, S.1    Christian, J.L.2    Moon, R.T.3    Melton, D.A.4
  • 14
    • 0026647447 scopus 로고
    • A. P. McMahon and R. T. Moon, Cell 58, 1075 (1989); W. C. Smith and R. M. Harland, ibid. 67, 753 (1991); S. Sokol, J. L. Christian, R. T. Moon, D. A. Melton, ibid., p. 741; A. Chakrabarti, G. Matthews, A. Colman, L. Dale, Development 115, 355 (1992); S. L. Wolda, C. J. Moody, R. T. Moon, Dev. Biol. 155, 46 (1993).
    • (1992) Development , vol.115 , pp. 355
    • Chakrabarti, A.1    Matthews, G.2    Colman, A.3    Dale, L.4
  • 15
    • 0027419249 scopus 로고
    • A. P. McMahon and R. T. Moon, Cell 58, 1075 (1989); W. C. Smith and R. M. Harland, ibid. 67, 753 (1991); S. Sokol, J. L. Christian, R. T. Moon, D. A. Melton, ibid., p. 741; A. Chakrabarti, G. Matthews, A. Colman, L. Dale, Development 115, 355 (1992); S. L. Wolda, C. J. Moody, R. T. Moon, Dev. Biol. 155, 46 (1993).
    • (1993) Dev. Biol. , vol.155 , pp. 46
    • Wolda, S.L.1    Moody, C.J.2    Moon, R.T.3
  • 20
    • 84920294710 scopus 로고    scopus 로고
    • note
    • All fz cDNAs were subcloned in the pRK5 vector [C. Gorman, D. R. Gies, G. McCray, DNA Prot. Eng. Techn. 2, 3 (1990)] with an optimal Kozak consensus sequence for translation at the initiator ATG (CCAC-CATG, preceded by different restriction sites for subcloning) and with different lengths of 3′ untranslated regions. The details of each construct are available upon request. Xwnt-5A was in pSP64 vector as described (8). All RNAs for injection were synthesized as capped transcripts in vitro with SP6 RNA polymerase (Ambion Megascript). Unless otherwise specified, RNAs were injected into the two cells near the equatorial midline region at the four-cell stage. Embryo preparation and staging were performed as described (24).
  • 21
    • 84920294709 scopus 로고    scopus 로고
    • note
    • Percentages of axis duplication were obtained by dividing the total number of embryos with a duplicated axis by the total number of embryos, scored from two to six independent experiments. Extensive axis duplication is defined by the presence of the cement gland and at least one eye in the duplicated axis.
  • 23
    • 0027370410 scopus 로고
    • B. J. Gavin, J. A. McMahon, A. P. McMahon, Genes Dev. 4, 2319 (1990); C. C. Clark et al., Genomics 18, 249 (1993).
    • (1993) Genomics , vol.18 , pp. 249
    • Clark, C.C.1
  • 24
    • 0028306459 scopus 로고    scopus 로고
    • note
    • Murine wnt-5 cDNA (14) was cloned in pCS2+ vector [D. L. Turner and H. Weintraub, Genes Dev. 8, 1434 (1994)] as the Eco RI and Xba I fragment, which contains about 60 bp 5′ and 360 bp 3′ untranslated regions.
  • 25
    • 84920294707 scopus 로고    scopus 로고
    • note
    • Some variability exists in the completeness of duplicated axes or axial rescue in UV-treated embryos among different batches of embryos. One possibility is that the coinjection of Xwnt-5A and hfz5 RNAs inevitably causes more variable results than does injection of a single RNA species, because the two proteins derived from the injected RNAs must be translated, processed, and localized properly to function together. In injection of a single RNA, such as one for Xwnt-8, the variables are only associated with the Xwnt-8 protein, because one or more receptors are provided endogenously. Another possibility is that Xwnt-5A inhibits gastrulation movements in gastrula stages (8); this later function of Xwnt-5A might affect the completeness of the axis to a variable degree.
  • 26
    • 84920294706 scopus 로고    scopus 로고
    • X. He et al., data not shown
    • X. He et al., data not shown.
  • 29
    • 84920294705 scopus 로고    scopus 로고
    • note
    • Embryo preparation, staging, UV irradiation, fixation and sectioning, and whole-mount in situ hybridization were performed as described (24).
  • 32
    • 0028935672 scopus 로고
    • S. B. Pierce and D. Kimelman, Development 121, 755 (1995); I. Dominguez, K. Itoh, S. Y. Sokol, Proc. Natl. Acad. Sci. U.S.A. 92, 8498 (1995).
    • (1995) Development , vol.121 , pp. 755
    • Pierce, S.B.1    Kimelman, D.2
  • 35
    • 84920294704 scopus 로고    scopus 로고
    • note
    • hfz5N was generated by introduction of a stop codon just before the first putative transmembrane helix (changing the preceding amino acids 237 and 238 from phenylalanine and tryptophan to threonine and arginine, respectively). The corresponding DNA fragment was subcloned in pCS2+.
  • 36
    • 84920294703 scopus 로고    scopus 로고
    • note
    • The authors are grateful to R. Moon for Xwnt-5A plasmid. We thank B. Huang for technical help and B. Williams, J. Debnath, P. Schwartzberg, and M. Rebagliati for discussion and comments on the manuscript. X.H. is supported by the U.S. Army Breast Cancer Research Program. J.N. is an investigator of the Howard Hughes Medical Institute.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.