Reproducibility of human sperm DNA measurements using the alkaline single cell gel electrophoresis assay

Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis

Volumn 374, Issue 2, 1997, Pages 261-268

  Hughes, Ciara M ^a   Lewis, Sheena E M ^a   McKelvey Martin, Valerie J ^b   Thompson, W ^a  

^a QUEEN'S UNIVERSITY BELFAST   (United Kingdom)

^b UNIVERSITY OF ULSTER   (United Kingdom)

Author keywords

Comet assay; DNA damage; Human spermatozoon; Reproducibility

Indexed keywords

ARTICLE; BIOASSAY; DNA DAMAGE; GEL ELECTROPHORESIS; HUMAN; HUMAN CELL; MALE; PRIORITY JOURNAL; REPRODUCIBILITY; SPERMATOZOON; X RAY;

DNA; DNA DAMAGE; ELECTROPHORESIS, AGAR GEL; HUMANS; MALE; REPRODUCIBILITY OF RESULTS; SOFTWARE; SPERMATOZOA; X-RAYS;

EID: 0030936364     PISSN: 00275107     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0027-5107(96)00241-2     Document Type: Article

References (11)

1. Hughes, C.M., Lewis, S.E.M., McKelvey-Martin, V.J. and Thompson, W., A comparison of baseline and induced DNA damage in human sperm from fertile and infertile man, using a modified comet assay, Mol. Hum. Reprod., in press.
2. Singh, N.P., McCoy, M.T., Tice, R. and Schneider, E.L. (1988) A simple technique for quantitation of low levels of DNA damage in individual cells, Exp. Cell Res., 175, 184-191.
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6. Ostling, O. and Johanson, K.J. (1984). Microelectrophoretic study of radiation induced DNA damage in individual cells, Biochem. Biophys. Res. Commun., 123, 291-298.
7. World Health Organization (WHO) (1992) Laboratory Manual for the Examination of Human Semen and Sperm-Cervical Mucus Interaction, University Press, Cambridge, p. 107.
8. Biggers, J.D., Whitten, K.K. and Whittingham, D.G. (1971) The culture of mouse embryos in vitro. In: J.C. David (Eds.), Methods in Mammalian Embryology, Freeman Press, San Francisco, pp. 86-94.
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