Defined epitopes: In vivo and in vitro studies using recombinant allergens

International Archives of Allergy and Immunology

Volumn 113, Issue 1-3, 1997, Pages 102-104

  Chapman, Martin D ^a   Smith, Alisa M ^a   Vailes, Lisa D ^a   Arruda, Karla ^a  

^a UNIVERSITY OF VIRGINIA   (United States)

Author keywords

Allergen; Asthma; Cockroach; Epitopes; Mite; Molecular biology

Indexed keywords

ALLERGEN; EPITOPE; IMMUNOGLOBULIN E;

COCKROACH; CONFERENCE PAPER; EXPRESSION VECTOR; HOUSE DUST ALLERGY; HUMAN; IMMUNOASSAY; INSECT ALLERGY; MITE; PRIORITY JOURNAL; SKIN TEST;

ALLERGENS; ANIMALS; COCKROACHES; EPITOPES; HUMANS; MITES; RECOMBINANT PROTEINS; SKIN TESTS;

EID: 0030931343     PISSN: 10182438     EISSN: 14230097     Source Type: Journal    
DOI: 10.1159/000237518     Document Type: Article

References (8)

1. Scheiner O, Kraft DG: Basic and practical aspects of recombinant allergens, Allergy 1995: 50:384-391.
2. Arruda LK, Vailcs LD, Mann BJ, Shannon J, Fox JW, Vedvick TS, Hayden ML, Chapman MD: Molecular cloning of a major cockroach (Blallella germanica) allergen, Bla g2: Sequence homology to the aspartic proteases, J Biol Chem 1995;270:19563-19568.
3. Arruda LK, Vailes LD, Hayden ML, Benjamin DC, Chapman MD: Molecular cloning of cockroach allergen, Bla g4. identifies ligand binding proteins (or calycins) as a cause of IgE antibody responses, J Biol C'hem 1995:270:31196-31201.
4. Arruda LK, Vailes LD, Platts-Mills TAE, Fcr-nandcz-Caldas E, Montealcgrc F, Lin KL, Chua KY, Rizzo MC, Naspitz CK, Chapman MD: Sensitization to Blomia tropicalis in patients with asthma and identification of allergen Bio 15. Am J Respic Crit Care Med, in press
5. Lin KL, Hsich KS, Thomas WR, Chiang BL, Chua KY: Characterization of Der p V allergen, cDNA analysis, IgE mediated reactivity to the recombinant protein, J Allergy Clin Immunol 1994;94:989-996.
6. Smith AM, Chapman MD: Reduction in IgE binding to allergen variants generated by site-directed mutagenesis: Contribution of disulfide bonds to the antigenic structure of the major house dust mite allergen, Der p 2. Mol Immunol 1995:33:399-405.
7. Smith AM, Chapman MD: Localization of antigen c sites on Der p 2 using oligonucleotide directed mutagenesis targeted to predicted surface residues, Clin Exp Allergy, in press
8. Smith AM, Taketomi EA, Mueller GA, Rule GS, Benjamin DC, Chapman MD: Antigenic and molecular structure of the mite allergen Der p2. Int Arch Allergy Immunol 1997; 113:99- 101