Pharmacological and structural integrity of muscarinic M, acetylcholine receptors produced in Sf9 insect cells

European Journal of Pharmacology

Volumn 333, Issue 2-3, 1997, Pages 269-278

  Weill, Claire ^a   Autelitano, François ^a   Guenet, Chantal ^b   Heitz, Freddy ^b   Goeldner, Maurice ^a   Ilien, Brigitte ^a  

^a CNRS   (France)

^b SANOFI   (France)

Author keywords

Aryldiazonium salt; Covalent labelling; Muscarinic receptor; Muscarinic receptor, m2 subtype; SF9 cells

Indexed keywords

MUSCARINIC M2 RECEPTOR;

ANIMAL CELL; ARTICLE; BACULOVIRUS; BINDING AFFINITY; BINDING SITE; CONTROLLED STUDY; ELECTROPHORESIS; HUMAN; HUMAN TISSUE; INSECT; LIGAND BINDING; MOLECULAR WEIGHT; NONHUMAN; PHOTOAFFINITY LABELING; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION;

ANIMALS; BACULOVIRIDAE; CLONING, MOLECULAR; DNA, COMPLEMENTARY; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HUMANS; INSECTS; MUSCARINIC AGONISTS; MUSCARINIC ANTAGONISTS; PEPTIDE FRAGMENTS; PHOTOAFFINITY LABELS; RECEPTOR, MUSCARINIC M2; RECEPTORS, MUSCARINIC; RECOMBINANT PROTEINS;

EID: 0030931103     PISSN: 00142999     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0014-2999(97)01139-4     Document Type: Article

References (50)

1. Autelitano F., Labat-Alcaraz M.-L., Sobrio F., Goeldner M., Ilien B. Synthesis and purification of highly tritiated aryldiazonium photoprobes for cholinergic binding sites. J. Label. Compd. Radiopharm. XXXVIII:1996;567-578.
2. Autelitano F., Weill C., Goeldner M., Ilien B. Covalent labelling of muscarinic acetylcholine receptors by tritiated aryldiazonium photoprobes. Biochem. Pharmacol. 53:1997;501-510.
3. Baldwin J.M. The probable arrangement of the helices in G protein-coupled receptors. EMBO. 12:1993;1693-1703.
4. Baldwin J.M. Structure and function of receptors coupled to G proteins. Curr. Opin. Cell. Biol. 6:1994;180-190.
5. Ballesteros J.A., Weinstein H. Integrated methods for the construction of three dimensional models and computational probing of structure-function relations in G protein coupled receptors. Methods Neurosci. 25:1995;366-428.
6. q/11, and phospholipase C-beta1. J. Biol. Chem. 267:1992;8081-8088.
7. Caulfield M.P. Muscarinic Receptors - Characterization, coupling and function. Pharmac. Ther. 58:1993;319-379.
8. Chen G.-Q., Gouaux J.E. Overexpression of bacterio-opsin in Escherichia coli as a water-soluble fusion to maltose binding protein: Efficient regeneration of the fusion protein and selective cleavage with trypsin. Protein Sci. 5:1996;456-467.
9. 50) of an enzymatic reaction. Biochem. Pharmacol. 22:1973;3099-3108.
10. Curtis C.A.M., Wheatley M., Bansal S., Birdsall N.J.M., Eveleigh P., Pedder E.K., Poyner D., Hulme E.C. Propylbenzilylcholine mustard labels an acidic residue in transmembrane helix 3 of the muscarinic receptor. J. Biol. Chem. 264:1989;489-495.
11. Dennis M., Giraudat J., Kotzyba-Hibert F., Goeldner M., Hirth C., Chang J.-Y., Lazure C., Chretien M., Changeux J.-P. Amino acids of the Torpedo marmorata acetylcholine receptor alpha subunit labeled by a photoaffinity ligand for the acetylcholine binding site. Biochemistry. 27:1988;2346-2357.
12. Dong G.Z., Kameyama K., Rinken A., Haga T. Ligand binding properties of muscarinic acetylcholine receptor subtypes (m1-m5) expressed in baculovirus-infected insect cells. J. Pharmacol. Exp. Ther. 274:1995;378-384.
13. Fraser C.M., Lee N.H., Pellegrino S.M., Kerlavage A.R. Molecular properties and regulation of G-protein-coupled receptors. Prog. Nucleic Acid Res. Mol. Biol. 49:1994;113-156.
14. Galzi J.-L., Revah F., Black D., Goeldner M., Hirth C., Changeux J.-P. Identification of a novel amino acid alpha-tyrosine 93 within the cholinergic ligands-binding sites of the acetylcholine receptor by photoaffinity labeling. J. Biol. Chem. 265:1990;10430-10437.
15. Gorissen H., Aerts G., Ilien B., Laduron P. Solubilization of muscarinic acetylcholine receptors from mammalian brain: An analytical approach. Anal. Biochem. 111:1981;33-34.
16. Grisshammer R., Tate C.G. Overexpression of integral membrane proteins for structural studies. Q. Rev. Biophys. 28:1995;315-422.
17. Haga, T., Haga, K., Hulme, E.C., 1990. Solubilization, purification and molecular characterization of muscarinic acetylcholine receptors. In: Hulme, E.C. (Ed.), Receptor Biochemistry, A Practical Approach. IRL Press, Oxford, pp. 51-78.
18. Harel M., Schalk I., Ehret-Sabatier L., Bouet F., Goeldner M., Hirth C., Axelsen P.H., Silman I., Sussman J.L. Quaternary ligand binding to aromatic residues in the active-site gorge of acetylcholinesterase. Proc. Natl. Acad. Sci. USA. 90:1993;9031-9035.
19. 2 muscarinic receptors in Sf9 cells: Characterisation and reconstitution with G-proteins. J. Recept. Signal Transduction Res. 15:1995;55-70.
20. Heitz F., Nay C., Guenet C. Expression of functional human muscarinic m2 receptors in different insect cell lines. J. Recept. Signal Transduction Res. 17:1997;305-317.
21. Hulme E.C., Birdsall N.J.M., Buckley N.J. Muscarinic receptor subtypes. Annu. Rev. Pharmacol. Toxicol. 30:1990;633-673.
22. Ilien B., Hirth C. Direct and energy-transfer photolabelling of brain muscarinic acetylcholine receptors. Eur. J. Biochem. 183:1989;331-337.
23. Ilien B., Hirth C. Photoaffinity labelling of solubilized muscarinic acetylcholine receptors. Biochem. (Life Sci. Adv.). 10:1991;31-39.
24. Ilien B., Mejean A., Hirth C. New photoaffinity labels for rat brain muscarinic acetylcholine receptors. Biochem. Pharmacol. 38:1989;2879-2887.
25. Jenkins N., Parekh R.B., James D.C. Getting the glycosylation right: Implications for the biotechnology industry. Nature Biotechnol. 14:1996;975-981.
26. Kameyama K., Haga K., Haga T., Moro O., Sadée W. Activation of a GTP-binding protein and a GTP-binding-protein-coupled receptor kinase (beta-adrenergic-receptor kinase-1) by a muscarinic receptor m2 mutant lacking phosphorylation sites. Eur. J. Biochem. 226:1994;267-276.
27. Kobilka B.K., Kobilka T.S., Daniel K., Reagan J.W., Caron M.G., Lefkowitz R.J. Chimeric alpha2-, beta-2-adrenergic receptors: Delineation of domains involved in effector coupling and ligand binding specificity. Science. 240:1988;1310-1316.
28. Kotzyba-Hibert F., Kapfer I., Goeldner M. Recent trends in photoaffinity labeling. Angew. Chem. Int. Ed. Engl. 34:1995;1296-1312.
29. Kubo T., Maeda A., Sugimoto K., Akiba I., Mikami A., Takahashi H., Haga T., Haga K., Ichiyama A., Kangawa K., Matsuo H., Hirose T., Numa S. Primary structure of porcine cardiac muscarinic acetylcholine receptor deduced from the cDNA sequence. FEBS Lett. 209:1986;367-372.
30. Kurtenbach E., Curtis C.A.M., Pedder E.K., Aitken A., Harris A.C.M., Hulme E.C. Muscarinic acetylcholine receptors - Peptide sequencing identifies residues involved in antagonist binding and disulfide bond formation. J. Biol. Chem. 265:1990;13702-13708.
31. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227:1970;680-685.
32. Maggio R., Vogel Z., Wess J. Reconstitution of functional muscarinic receptors by co-expression of amino and carboxy terminal receptor fragments. FEBS Lett. 319:1993;195-200.
33. Nakata H., Kameyama K., Haga K., Haga T. Location of agonist-dependent-phosphorylation sites in the third intracellular loop of muscarinic acetylcholine receptors (m2 subtype). Eur. J. Biochem. 220:1994;29-36.
34. Parker E.M., Kameyama K., Higashijima T., Ross E.M. Reconstitutively active G-protein-coupled receptors purified from baculovirus-infected insect cells. J. Biol. Chem. 266:1991;519-527.
35. Peterson G.L., Herron G.S., Yamaki M., Fullerton D.S., Schimerlik M.I. Purification of the muscarinic acetylcholine receptor from porcine atria. Proc. Natl. Acad. Sci. USA. 81:1984;4993-4997.
36. Richardson R.M., Kim C., Benovic J.L., Hosey M.M. Phosphorylation and desensitization of human m2 muscarinic cholinergic receptors by two isoforms of the beta-adrenergic receptor kinase. J. Biol. Chem. 268:1993;13650-13656.
37. Rinken A. Subtype-specific changes in ligand binding properties after solubilization of muscarinic receptors from baculovirus-infected Sf9 insect cell membranes. J. Pharmacol. Exp. Ther. 272:1995;8-14.
38. Rinken A., Kameyama K., Haga T., Engström L. Solubilization of muscarinic receptor subtypes from baculovirus infected Sf9 insect cells. Biochem. Pharmacol. 48:1994;1245-1251.
39. Rubenstein R.C., Wong S.K., Ross E.M. The hydrophobic tryptic core of the beta-adrenergic receptor retains Gs regulatory activity in response to agonists and thiols. J. Biol. Chem. 262:1987;16655-16662.
40. Savarese T.M., Fraser C.M. In vitro mutagenesis and the search for structure-function relationship among G protein-coupled receptors. Biochem. J. 283:1992;1-19.
41. Schalk I., Ehret-Sabatier L., Bouet F., Goeldner M., Hirth C. Trp279 is involved in the binding of quaternary ammonium at the peripheral site of Torpedo marmorata acetylcholinesterase. Eur. J. Biochem. 219:1994;155-159.
42. Schöneberg T., Liu J., Wess J. Plasma membrane localization and functional rescue of truncated forms of a G protein-coupled receptor. J. Biol. Chem. 270:1995;18000-18006.
43. Schwartz T.W. Locating ligand-binding sites in 7TM receptors by protein engineering. Curr. Opin. Biotechnol. 5:1994;434-444.
44. Strader C.D., Fong T.M., Tota M.R., Underwood D., Dixon R.A.F. Structure and function of G protein-coupled receptors. Annu. Rev. Biochem. 63:1994;101-132.
45. Tate C.G., Grisshammer R. Heterologous expression of G-protein-coupled receptors. TIBTECH. 14:1996;426-430.
46. Trumpp-Kallmeyer S., Hoflack J., Bruinvels A., Hibert M. Modeling of G-protein-coupled receptors: Application to dopamine, adrenaline, serotonine, acetylcholine, and mammalian opsin receptors. J. Med. Chem. 35:1992;3448-3462.
47. Tucker J., Grisshammer R. Purification of a rat neurotensin receptor expressed in Escherichia coli. Biochem. J. 317:1996;891-899.
48. Van Rhee A.M., Jacobson K.A. Molecular architecture of G protein-coupled receptors. Drug Dev. Res. 37:1996;1-38.
49. 3 muscarinic acetylcholine receptor produced in Sf9 insect cells using the baculovirus system. FEBS Lett. 283:1991;52-56.
50. Wess J. Minireview - Mutational analysis of muscarinic acetylcholine receptors: Structural basis of ligand/receptor/G-protein interactions. Life Sci. 53:1993;1447-1463.