Refinements in re-amplification and cloning of DDRT-PCR products

BioTechniques

Volumn 22, Issue 6, 1997, Pages 1048-1051

  Bhattacharjee, A ^a   Rutherford, M S ^a   Abrahamsen, M S ^a   Lappi, V R ^a   Schook, L B ^a,b  

^a UNIVERSITY OF MINNESOTA   (United States)

^b Dept of Veterinary PathoBiology ^*  (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; BACTERIUM COLONY; GENE AMPLIFICATION; GENE EXPRESSION; MOLECULAR CLONING; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

BACTERIA (MICROORGANISMS);

EID: 0030926948     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/97226bm08     Document Type: Article

References (6)

1. Costa, G.L., A. Grafsky and M.P. Weiner. 1994. Cloning and analysis of PCR-generated DNA fragments. PCR Methods Appl. 3:338-345.
2. Hadjeb, N. and G.A. Berkowitz. 1996. Preparation of T-overhang vectors with high PCR product cloning efficiency. BioTechniques 20:20-22.
3. Horn, T.L., M.S. Rutherford and L.B. Schook. 1996. Alteration of proinflammatory gene expression and hepatic macrophage populations following dimethylnitrosamine exposure in vivo. Toxicologist 30:321.
4. Liang, P., L. Averboukh and A.B. Pardee. 1993. Distribution and cloning of eukaryotic mRNAs by means of differential display: refinements and optimization. Nucleic Acids Res. 14:3269-3275.
5. Marchuk, D., M. Drum, A. Saulino and F.S. Collins. 1991. Construction of T-vectors, a rapid and general system for direct cloning of unmodified PCR products. Nucleic Acids Res. 19:1154.
6. Raju, R., B.L. Hoppe, D. Navaneetham and B.M. Conti-Fine. 1995. Rapid method for the elution and analysis of PCR products separated on high resolution acrylamide gels. BioTechniques 18:32-36.