Preparation of T-overhang vectors with high PCR product cloning efficiency

BioTechniques

Volumn 20, Issue 1, 1996, Pages 20-22

  Hadjeb, Noureddine ^a   Berkowitz, Gerald A ^a  

^a RUTGERS UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA FRAGMENT; LIGASE;

ARTICLE; CLONING VECTOR; LIGATION; POLYMERASE CHAIN REACTION; TECHNIQUE; BIOTECHNOLOGY; GENE VECTOR; GENETICS; METHODOLOGY; MOLECULAR CLONING; PLASMID;

BIOTECHNOLOGY; CLONING, MOLECULAR; GENETIC VECTORS; PLASMIDS; POLYMERASE CHAIN REACTION;

EID: 0029665022     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/96201bm02     Document Type: Article

References (6)

1. Aslanidis, C. and P.J. de Jong. 1990 Ligation-independent cloning of PCR products (LIC-PCR). Nucleic Acids Res. 18:6069-6074.
2. Clarck, J.M. 1988. Novel non-templated nucleotide addition reactions catalized by procariotic and eucariotic DNA polymerases. Nucleic Acids Res. 16:9677-9686.
3. Kaufman, D.L. and G.A. Evans. 1990. Restriction endonuclease cleavage at the termini of PCR products. BioTechniques 9:304-305.
4. Marchuck, D., M. Drum, A. Saulino and F.S. Collins. 1991. Construction of T-vectors, a rapid and general system for direct cloning of unmodified PCR products. Nucleic Acids Res. 19:1154.
5. Scharf, S.J., G.T. Horn and H.A. Erlich. 1986. Direct cloning and sequence analysis of enzymatically amplified genomic sequences. Science 233:1076-1078.
6. Shuldiner, A.R., L.A. Scott and J. Roth. 1990. PCR-induced (ligase free) subcloning, a rapid reliable method to subclone polymerase chain reaction (PCR) products. Nucleic Acids Res. 18:1920.