Truncation of the N- and C-terminal regions of the human 11β-hydroxysteroid dehydrogenase type 2 enzyme and effects on solubility and bidirectional enzyme activity

Molecular and Cellular Endocrinology

Volumn 131, Issue 2, 1997, Pages 173-182

  Obeyesekere, Varuni R ^a   Li, Kevin X Z ^a   Ferrari, Paolo ^a   Krozowski, Zygmunt ^a  

^a BAKER IDI HEART AND DIABETES INSTITUTE   (Australia)

Author keywords

11 hydroxysteroid dehydrogenase type II; Dexamethasone; Glucocorticoid; Oxidoreductase

Indexed keywords

11BETA HYDROXYSTEROID DEHYDROGENASE; DEXAMETHASONE;

AMINO TERMINAL SEQUENCE; ANIMAL CELL; ARTICLE; CARBOXY TERMINAL SEQUENCE; ENDOPLASMIC RETICULUM; ENZYME ACTIVITY; ENZYME STABILITY; MAMMAL CELL; NONHUMAN; PRIORITY JOURNAL; SOLUBILITY;

11-BETA-HYDROXYSTEROID DEHYDROGENASES; AMINO ACID SEQUENCE; ANIMALS; BLOTTING, WESTERN; CELL MEMBRANE; CHO CELLS; CRICETINAE; CROSS-LINKING REAGENTS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HUMANS; HYDROCORTISONE; HYDROXYSTEROID DEHYDROGENASES; ISOENZYMES; KINETICS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PEPTIDE FRAGMENTS; PROTEIN STRUCTURE, SECONDARY; SOLUBILITY; STRUCTURE-ACTIVITY RELATIONSHIP;

EID: 0030921736     PISSN: 03037207     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0303-7207(97)00106-8     Document Type: Article

References (41)

1. Agarwal, A.K., Rogerson, F.M., Mune, T., White, P.C., 1995. Analysis of the human gene encoding the kidney isozyme of 11β-hydroxysteroid dehydrogenase. J. Steroid Biochem. Mol. Biol. 55, 473-479.
2. Albiston, A.L., Obeyesekere, V.R., Smith, R.E., Krozowski, Z.S., 1994. Cloning and tissue distribution of the human 11β-hydroxysteroid dehydrogenase type 2 enzyme. Mol. Cell. Endocrinol. 105, R11-R17.
3. Alvarez, C.I., Genti-Raimondi, S., Patrito, L.C., Flury, A., 1994. Topography of human placental 3β-hydroxysteroid dehydrogenase/δ 5-4 isomerase in microsomal membrane. A study using limited proteolysis and immunoblotting. Biochim. Biophys. Acta 1207, 102-108.
4. Benediktsson, R., Lindsay, R.S., Noble, J., Seckl, J.R., Edwards, C., 1993. Glucocorticoid exposure in utero - new model for adult hypertension. Lancet 341, 339-341.
5. Bradford, M., 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilising the principle of protein dye binding. Anal. Biochem. 72, 248-254.
6. Chou, P.Y., Fasman, G.D., 1978. Prediction of the secondary structure of proteins from their amino acid sequence. Adv. Enzymol. 47, 45-148.
7. Cole, T.J., 1995. Cloning of the mouse 11β-hydroxysteroid dehydrogenase type 2 gene: tissue specific expression and localization in distal convoluted tubules and collecting ducts of the kidney. Endocrinology 136, 4693-4696.
8. Diedrich, S., Hanke, B., Bahr, V., Oelkers, W., 1996. The metabolism of 9α fluorinated steroids in the human kidney. Endocr. Res. 22, 803-810.
9. Edwards, C.R., Stewart, P.M., Burt, D., Brett, L., McIntyre, M.A., Sutanto, W.S., deKloet, E.R., Monder, C., 1988. Localisation of 11β-hydroxysteroid dehydrogenase-tissue specific protector of the mineralocorticoid receptor. Lancet 2, 986-989.
10. Emini, E.A., Hughes, J., Perlow, D., Boger, J., 1985. Induction of hepatitia A virus-inducing antibody by a virus specific synthetic peptide. J. Virol. 55, 836-839.
11. Ferrari, P., Obeyesekere, V.R., Li, K., Andrews, R.K., Krozowski, Z.S., 1996a. The 11β-hydroxysteroid dehydrogenase type II enzyme: Biochemical consequences of the congenital R337C mutation. Steroids 61, 197-200.
12. Ferrari, P., Obeyesekere, V.R., Li, K., Wilson, R.C., New, M.I., Funder, J.W., Krozowski, Z.S., 1996b. Point mutations abolish 11β-hydroxysteroid dehydrogenase type II activity in three families with the congenital syndrome of apparent mineralocorticoid excess. Mol. Cell. Endocrinol. 119, 21-24.
13. Funder, J.W., Pearce, P.T., Smith, R., Smith, A.I., 1988. Mineralocorticoid action: target tissue specificity is enzyme, not receptor, mediated. Science 242, 583-585.
14. Ghosh, D., Weeks, C.M., Grochulski, P., Duax, W.L., Erman, M., Rimsay, R.L., Orr, J.C., 1991. Three-dimensional structure of holo 3α,20β-hydroxysteroid dehydrogenase: a member of a short-chain dehydrogenase family. Proc. Natl. Acad. Sci. USA 88, 10064-10068.
15. Heffernan, M., Dennis, J.W., 1991. Polyoma and hamster papovavirus large T antigen-mediated replication of expression shuttle vectors in Chinese hamster ovary cells. Nucleic Acids Res. 19, 85-92.
16. Hofmann, K., Stoffel, W., 1993. TMBASE - A database of membrane spanning protein segments. Biol. Chem. Hoppe-Seyler 374, 166.
17. Hopp, T.P., Woods, K.R., 1981. Prediction of protein antigenic determinants for amino acid sequences. Proc. Natl. Acad. Sci. USA 78, 3824-3828.
18. Jornvall, H., Persson, B., Krook, M., Atrian, S., Gonzalezduarte, R., Jeffery, J., Ghosh, D., 1995. Short-chain dehydrogenases reductases (SDR). Biochemistry 34, 6003-6013.
19. Krozowski, Z., 1992. 11β-hydroxysteroid dehydrogenase and the short-chain alcohol dehydrogenase (SCAD) superfamily. Mol. Cell. Endocrinol. 84, C25-C31.
20. Krozowski, Z., Obeyesekere, V., Smith, R., Mercer, W., 1992. Tissue-specific expression of an 11β-hydroxysteroid dehydrogenase with a truncated N-terminal domain. A potential mechanism for differential intracellular localization within mineralocorticoid target cells. J. Biol. Chem. 267, 2569-2574.
21. Larson, J.R., Coon, M.J., Porter, T.D., 1991. Alcohol-inducible Cytochrome P450IIE1 lacking the hydrophobic NH2-terminal segment retains catalytic activity and is membrane-bound when expressed in Escherichia coli. J. Biol. Chem. 266, 7321-7324.
22. Lakshmi, V., Monder, C., 1988. Purification and characterization ofthe corticosteroid 11β-dehydrogenase component of the rat liver 11β-hydroxysteroid dehydrogenase complex. Endocrinology 123, 2390-2398.
23. Li, K.X.Z., Smith, R.E., Ferrari, P., Funder, J.W., Krozowski, Z.S., 1996. Rat 11β-hydroxysteroid dehydrogenase type 2 enzyme is expressed at low levels in the placenta and is modulated by adrenal steroids in the kidney. Mol. Cell. Endocrinol. 120, 67-75.
24. Mune, T., Rogerson, F.M., Nikkila, H., Agarwal, A.K., White, P.C., 1995. Human hypertension caused by mutations in the kidney isozyme of 11β-hydroxysteroid dehydrogenase. Nat. Genet. 10, 394-399.
25. Mune, T., White, P.C., 1996. Apparent mineralocorticoid excess: genotype is correlated with biochemical phenotype. Hypertension 27, 1193-1199.
26. Naray-Fejes-Toth, A., Fejes-Toth, G., 1995. Expression cloning of the aldosterone target cell-specific 11β-hydroxysteroid dehydrogenase from rabbit collecting duct cells. Endocrinology 136, 2579-2586.
27. Naray-Fejes-Toth, A., Fejes-Toth, G., 1996. Subcellular localization of the type 2 11β-hydroxysteroid dehydrogenase - A green fluorescent protein study. J. Biol. Chem. 271, 15436-15442.
28. Naray-Fejes-Toth, A., Rusvai, E., Denault, D.L., Stgermain, D.L., Fejes-Toth, G., 1993. Rapid communication - Expression and characterization of a new species of 11β-hydroxysteroid dehydrogenase in Xenopus Oocytes. Am J Physiol 265, F896-F900.
29. Obeid, J., Curnow, K.M., Aisenberg, J., White, P.C., 1993. Transcripts originating in Intron-1 of the HSDIl (11β- hydroxysteroid dehydrogenase) gene encode a truncated polypeptide that is enzymatically inactive. Mol. Endocrinol. 7, 154-160.
30. m 11β-hydroxysteroid dehydrogenase type II enzyme in a family with apparent mineralocorticoid excess. J. Clin. Endocrinol. Metab. 80, 3381-3383.
31. Ocheretina, O., Harnecker, J., Rother, T., Schmid, R., Scheibe, R., 1993. Effects of N-terminal truncations upon chloroplast NADP-malate dehydrogenases from pea and spinach. Biochim. Biophys. Acta 1163, 10-16.
32. Oelkers, W., Buchen, S., Diederich, S., Krain, J., Muhme, S., Schoneshofer, M., 1994. Impaired renal 11β-oxidation of 9α-fluorocortisol: an explanation for its mineralocorticoid potency. J. Clin. Endocrinol. Metab. 78, 928-932.
33. Ozols, J., 1995. Lumenal orientation and post-translational modifications of the liver microsomal 11β-hydroxysteroid dehydrogenase. J. Biol. Chem. 270, 2305-2312.
34. Roitelman, J., Olender, E.H., Bar, N.S., Dunn, W.J., Simoni, R.D., 1992. Immunological evidence for eight spans in the membrane domain of 3-hydroxy-3-methylglutaryl coenzyme A reductase: implications for enzyme degradation in the endoplasmic reticulum. J. Cell Biol. 117, 959-973.
35. Smith, R.E., Li, K.X., Andrews, R.K., Krozowski, Z.S., 1997. Immunohistochemical and molecular characterization of the rat 11β-hydroxysteroid dehydrogenase type II enzyme. Endocrinology 138, 540-547.
36. Stewart, P.M., Krozowski, Z.S., Gupta, A., Milford, D.V., Howie, A.J., Sheppard, M.C., Whorwood, C.B., 1996. Hypertension in the syndrome of apparent mineralocorticoid excess due to mutation of the 11β-hydroxysteroid dehydrogenase type 2 gene. Lancet 347, 88-91.
37. Stewart, P.M., Wallace, A.M., Valentino, R., Burt, D., Shackle-ton, C.H., Edwards, C.R.W., 1987. Mineralocorticoid activity of liquorice: 11β-hydroxysteroid dehydrogenase comes of age. Lancet 2, 821-824.
38. Ulick, S., Levine, S.L., Gunczler, P., Zanconato, G., Ramirez, L.C., Rauh, W., Rosler, A., Bradlow, L.H., New, M.I., 1979. A syndrome of apparent mineralocorticoid excess associated with defects in the peripheral metabolism of cortisol. J. Clin. Endocrinol. Metab. 49, 757-763.
39. Voice, M.W., Seckl, J.R., Edwards, C.R.W., Chapman, K.E., 1996. 11β-hydroxysteroid dehydrogenase type 1 expression in 2S FAZA hepatoma cells is hormonally regulated: A model system for the study of hepatic glucocorticoid metabolism. Biochem. J. 317, 621-625.
40. Wilson, R.C., Krozowski, Z.S., Li, K., Obeyesekere, V.R., Razzaghyazar, M., Harbison, M.D., Wei, J.Q., Shackleton, C., Funder, J.W., New, M.I., 1995. A mutation in the HSD11β2 gene in a family with apparent mineralocorticoid excess. J. Clin. Endocrinol. Metab. 80, 2263-2266.
41. Zhou, M.Y., Gomez-Sanchez, E.P., Cox, D.L., Cosby, D., Gomez-Sanchez, C.E., 1995. Cloning, expression, and tissue distribution of the rat nicotinamide adenine dinucleotide-dependent 11β- hydroxysteroid dehydrogenase. Endocrinology 136, 3729-3734.